Association of interleukin-10 gene promoter polymorphisms with susceptibility to acute pyelonephritis in children

Abstract

Interleukin-10 (IL-10) is a potent inhibitor of leukocyte chemotaxis, bacterial killing in phagocytes and synthesis of pro-inflammatory cytokines and chemokines, and recent studies have suggested an important role for this immunoregulatory cytokine in the pathogenesis of urinary tract infections (UTIs). Therefore, the gene encoding IL-10 (IL10) is an attractive candidate for association studies attempting to identify susceptibility genes conferring risk of UTIs. In this case-control study, we aimed to investigate the association of single nucleotide polymorphisms (SNPs) in the promoter region of IL10 with acute pyelonephritis in the Slovak population. Polymerase chain reaction with sequence-specific primers was used to analyse IL10 -1082A/G (rs1800896), -819C/T (rs1800871) and -592C/A (rs1800872) SNPs in 147 children with acute pyelonephritis and 215 healthy controls. Comparison of patients with healthy controls using the logistic regression analysis revealed significantly increased risk of developing recurrent attacks of acute pyelonephritis for -1082 G allele in a dominant genetic model GG (GG + AG vs. AA, P = 0.019, odds ratio (OR) = 2.26). A similar tendency was also found when the recurrent acute pyelonephritis subgroup was compared to episodic pyelonephritis cases (GG + AG vs. AA, P = 0.009, OR = 3.38). In conclusion, our results suggest that IL10 -1082 A/G SNP is a susceptibility factor for development of recurrent attacks of acute pyelonephritis.

Urinary Proteins, Vitamin D and Genetic Polymorphisms as Risk Factors for Febrile Urinary Tract Infection and Relation with Bacteremia: A Case Control Study

“…Also in UTI genetic factors likely play a role, as illustrated by the finding that positive family history is a risk factor for recurrent UTI [ 23 ] and reviewed in [ 1 ]. Studies evaluating the role of genetic factors in UTI were mainly performed in children [ 24 – 29 ]. No studies have investigated the contribution of host genetic factors to the development of UTI complicated by bacteremia.…”

Role of interleukin-10 gene polymorphisms in the development of coronary artery disease in Chinese population

“…IL-10 is produced by T-lymphocytes, and is an important anti-inflammatory and immune-suppressive cytokine that regulates angiogenesis in many inflammation-related diseases (Bantis et al, 2008;Javor et al, 2014;Peng et al, 2015;Yu et al, 2015). The IL-10 gene is a candidate gene in the pathophysiological mechanism of auto-immune/inflammatory disease, as it can regulate both the cellular and humoral immunity.…”

Immunomodulation therapy offers new molecular strategies to treat UTI

“…Mechanisms of innate immune activation and essential effector functions in APN have been mapped using cellular infection technology, animal models and clinical studies 4 , 10 , 11 , 14 , 16 , 18 , 19 , 32 , 75 – 80 . Key regulators of UTI severity have been evaluated using gene knockout technology and their relevance to human disease has been verified in clinical studies 10 , 11 , 29 , 78 .…”