MicroRNA-490-3p inhibits proliferation of A549 lung cancer cells by targeting CCND1

Gu, Haihua; Yang, Tao; Fu, Shaozi; Chen, Xiaofan; Guo, Lei; Ni, Yiming

doi:10.1016/j.bbrc.2014.01.020

Cited by 48 publications

(39 citation statements)

References 31 publications

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“…Gu et al reported that miR-490-3p inhibits proliferation of A549 lung cancer cells [24], and Zhang et al showed that miR-490-3p modulates cell growth and epithelial to mesenchymal transition (EMT) of hepatocellular carcinoma cells [21]. These studies suggest us that miR-490-3P may also contribute to tumor development; however, its function in the development of drug resistance hasn’t been studied.…”

Section: Discussionmentioning

confidence: 99%

microRNA 490-3P enhances the drug-resistance of human ovarian cancer cells

Chen

Xiu

et al. 2014

J Ovarian Res

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BackgroundMicroRNAs (miRNAs) are non-coding, single-stranded small RNAs that regulate gene expression negatively, which is involved in fundamental cellular processes. In this study, we investigated the role of miR-490-3P in the development of drug resistance in ovarian cancer cells.MethodsThe human ovarian carcinoma cell line A2780 and A2780/Taxol were exposed to paclitaxel in the presence or absence of microRNA 490-3P transfection, after which cell viability were performed by CCK-8 assay. Reverse transcription polymerase chain reaction (RT-PCR) and western blotting were used to assess the mRNA and protein expression levels of GST-π, MDR1 or P-gp.ResultsOur results showed higher miR-490-3P mRNA expression level in A2780/Taxol cells than in A2780 cells (p < 0.05). Following miR-490-3P transfection, both A2780 and A2780/Taxol cells showed decreased sensitivity to paclitaxel. The mRNA expression levels of MDR1, GST-π (p < 0.05) and protein expression levels of P-gp, GST-π were down-regulated after miR-490-3P transfection in comparison to mock and negative control cancer cells.ConclusionOur results demonstrate for the first time that microRNA 490-3P may be involved in the development of drug resistance in ovarian cancer.Electronic supplementary materialThe online version of this article (doi:10.1186/s13048-014-0084-4) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

microRNA 490-3P enhances the drug-resistance of human ovarian cancer cells

Chen

Xiu

et al. 2014

J Ovarian Res

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“…Our findings revealed that epigenetic silencing of miR-490-3p promoted gastric tumorigenesis by upregulating SMARCD1, a member of the SWItch/Sucrose NonFermentable (SWI/SNF) chromatin remodeling family involved in cancer development (18). MiR-490-3p has been shown to exert tumor-suppressing actions in other types of cancer, such as colon and lung cancers (19,20). Here, we demonstrated that promoter DNA hypermethylation of its host gene CHRM2 might account for the downregulation of miR-490-3p in gastric cancer.…”

Section: Discussionmentioning

confidence: 82%

Epigenetic Silencing of miR-490-3p Reactivates the Chromatin Remodeler SMARCD1 to PromoteHelicobacter pylori–Induced Gastric Carcinogenesis

et al. 2015

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Chromatin remodeling has emerged as a hallmark of gastric cancer, but the regulation of chromatin regulators other than genetic change is unknown. Helicobacter pylori causes epigenetic dysregulation to promote gastric carcinogenesis, but the roles and functions of microRNAs (miRNA) in this multistage cascade are not fully explored. In this study, miRNA expression in preneoplastic and neoplastic lesions in murine stomachs induced by H. pylori and N-methyl-N-nitrosourea (MNU) was profiled by miRNA expression array. miR-490-3p exhibited progressive downregulation in gastritis, intestinal metaplasia, and adenocarcinoma during H. pylori and MNU-induced gastric carcinogenesis. Significant downregulation of miR-490-3p was confirmed in human gastric cancer tissues in which its regulatory region was found to be hypermethylated. miR-490-3p exerted growth-and metastasis-suppressive effects on gastric cancer cells through directly targeting SMARCD1, a SWItch/ Sucrose NonFermentable (SWI/SNF) chromatin remodeling complex subunit. Knockdown of SMARCD1 significantly attenuated the protumorigenic effects of miR-490-3p inhibitor, whereas enforced expression of SMARCD1 promoted in vitro and in vivo oncogenic phenotypes of gastric cancer cells. SMARCD1 was markedly upregulated in gastric cancer in which its high expression was associated with shortened patients' survival independent of TNM staging. In conclusion, hypermethylation-mediated silencing of miR-490-3p reactivates SMARCD1 to confer malignant phenotypes, mechanistically linking H. pylori, chromatin remodeling, and gastric carcinogenesis. Cancer Res; 75(4); 754-65. Ó2014 AACR.

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“…Accumulating evidence indicates that miRNAs are often dysregulated in human cancers and play oncogenic or tumor suppressive roles [9,10]. A variety of miRNAs have been shown to regulate NSCLC development, including miR-451, miR130a, miR-490-3p, miR-16, miR-205, and miR-486-5p [8,[11][12][13][14][15]. In particular, miR-342-3p is deregulated in several cancers, including NSCLC [16][17][18], and also functions as a tumor suppressor in colorectal and cervical cancers [17,18].…”

Section: Introductionmentioning

confidence: 99%

miR-342-3p targets RAP2B to suppress proliferation and invasion of non-small cell lung cancer cells

et al. 2015

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MicroRNAs (miRNAs) play critical roles in cancer development and progression. In this study, we examined the roles and molecular mechanisms of miR-342-3p in human non-small cell lung cancer (NSCLC). The results showed that miR-342-3p is downregulated in NSCLC cell lines and tissues, and its overexpression induces significant inhibition of NSCLC cell proliferation, invasion, and tumor growth in nude mice. In addition, miR-342-3p repressed RAP2B expression through interactions with its 3'-UTR region. Restoration of RAP2B expression reversed miR-342-3p-mediated inhibitory activity in NSCLC cells. Finally, analyses of miR-342-3p and RAP2B levels in NSCLCs revealed that miR-342-3p inversely correlated with RAP2B mRNA expression. Our collective findings provide preliminary evidence that miR-342-3p acts as a tumor suppressor in NSCLC through repression of RAP2B.

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MicroRNA-490-3p inhibits proliferation of A549 lung cancer cells by targeting CCND1

Cited by 48 publications

References 31 publications

microRNA 490-3P enhances the drug-resistance of human ovarian cancer cells

microRNA 490-3P enhances the drug-resistance of human ovarian cancer cells

Epigenetic Silencing of miR-490-3p Reactivates the Chromatin Remodeler SMARCD1 to PromoteHelicobacter pylori–Induced Gastric Carcinogenesis

miR-342-3p targets RAP2B to suppress proliferation and invasion of non-small cell lung cancer cells

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