Pure natural orifice transluminal endoscopic surgery (NOTES) involving peroral endoscopic salpingo‐oophorectomy (POESY)

Hornemann, Amadeus; Suetterlin, Marc; Trunk, Marcus J.; Gerhardt, Axel; Kaehler, Georg

doi:10.1016/j.ijgo.2013.08.024

Cited by 5 publications

(2 citation statements)

References 9 publications

(8 reference statements)

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“…Although our study focused on an innovative method, we try to strip the fallopian tube mucosa absolutely out of the whole fallopian tube tissue and then have a digestion of the stripped fallopian tube mucosa to isolate the MSCs, and we suggest that this cell source is suitable for regenerative medicine and has a broader application than hFTMSCs. Fallopian tube mucosa can be obtained by the salpingo-scope, which is a less invasive procedure than tubal resection [ 23 ]. The vaginal route is the least invasive and most economical route and should be the first choice to obtain the hFMMSCs.…”

Section: Discussionmentioning

confidence: 99%

The utility of human fallopian tube mucosa as a novel source of multipotent stem cells for the treatment of autologous reproductive tract injury

Wang

Zhao

et al. 2015

Stem Cell Res Ther

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IntroductionFallopian tube, which is normally discarded in surgical procedures, has proven to be a source of mesenchymal stem cells (MSCs) with increasing evidence. However, fallopian tube mucosa, which can be acquired via non-invasive procedures, is a previously unknown source of MSCs. In the present study, we explored the existence of MSCs in the human fallopian tube mucosa and also compared multipotent stem cells derived from fallopian tubes and fallopian tube mucosa according to their biological characteristics and therapeutic potential for treatment of autologous reproductive tract injury.MethodsCells isolated from human fallopian tubes and fallopian tube mucosa were expanded and characterised by flow cytometry. The proliferative capacity of both cell types was measured by performing colony-forming unit-fibroblast and Cell Counting Kit-8 assays. Both cell types underwent in vitro adipogenic, chondrogenic, and osteogenic differentiation. The expression of osteocyte-, adipocyte-, and chondrocyte-related genes in the differentiated cell lineages was assessed by reverse transcription-polymerase chain reaction. The secretion of growth factors and immunomodulatory cytokines by both cell types were measured by enzyme-linked immunosorbent assays.ResultsWe found that MSCs existed in the fallopian tube mucosa. The comparison between human fallopian tube MSCs (hFTMSCs) and human fallopian tube mucosa MSCs (hFMMSCs) showed that hFTMSCs had a stronger proliferative capacity and shorter duplication time than hFMMSCs. Both cell types could be differentiated into adipocytes, osteoblasts, or chondrocytes in vitro. Real-time polymerase chain reaction analysis demonstrated that hFTMSCs displayed increased expression of osteogenic-specific genes compared with hFMMSCs, but the two types of cells showed no significant increase in the mRNA expression of adipogenic-specific or chondrogenic-specific genes. hFMMSCs and hFTMSCs robustly produced a variety of growth factors and immunomodulatory cytokines.ConclusionsHuman fallopian tube mucosa is a novel source of multipotent cells. hFMMSCs demonstrated stronger proliferative capacity and superior secretion of growth factors and immunomodulatory cytokines than hFTMSCs, making the former a better source of stem cells for the treatment of autologous reproductive tract injury. Compared with fallopian tube, fallopian tube mucosa has more wide-ranging applications and can be used to carry out autologous transplantation.

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Section: Discussionmentioning

confidence: 99%

The utility of human fallopian tube mucosa as a novel source of multipotent stem cells for the treatment of autologous reproductive tract injury

Wang

Zhao

et al. 2015

Stem Cell Res Ther

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“…Recently, transvaginal NOTES has been performed in cholecystectomy, appendectomy, 4 5 nephrectomy, 6 and gynecologic diseases. NOTES can be performed via a variety of approaches, including that through the stomach, 7 8 9 but the majority of NOTES procedures have been performed transvaginally. The vagina can be easily decontaminated, and it provides direct access.…”

Section: Introductionmentioning

confidence: 99%

Review of transvaginal natural orifice transluminal endoscopic surgery in gynecology

Yoshiki

2017

Gynecology and Minimally Invasive Therapy

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Recent technologic advances in endoscopic instrumentation and optics have allowed the development of a less invasive alternative to conventional laparoscopic surgery. During the past decade, natural orifice transluminal endoscopic surgery (NOTES) flourished in the field of general surgery, and it has emerged as a new concept of minimally invasive surgery. NOTES yields access to the abdominal cavity without any incisions on the abdominal wall (scarless surgery), and the natural orifices of the body surface, such as the mouth and the vagina, serve as the gateway to the peritoneal cavity. In gynecology, the vagina of a woman can be considered as an additional route for surgery. Recently, clinical application of transvaginal NOTES has broadened significantly in gynecology. Using transvaginal NOTES by applying the method of single-incision laparoscopic surgery via the vaginal route, not only adnexal surgery and hysterectomy, but also myomectomy and oncologic surgery could be performed safely and effectively in selected patients. In future, further studies should be conducted to evaluate the true clinical feasibility and safety of transvaginal NOTES.

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Per-oral endoscopic salpingo-oophorectomy (POESY) in humans: a prospective clinical feasibility study

Hornemann

Suetterlin

Kaehler

2017

Arch Gynecol Obstet

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Our study demonstrates the feasibility of the transgastric access to the pelvis. The gastroscope provided excellent optical control and good tissue preparation. Therefore, we conclude that pure NOTES procedures using the transgastric access to the adnexa are feasible. An increasing role of transgastric procedures for diseases in the pelvic region can be expected in particular if new endoscopic platforms with better means of instrumentation and tissue management become available.

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Pure natural orifice transluminal endoscopic surgery (NOTES) involving peroral endoscopic salpingo‐oophorectomy (POESY)

Cited by 5 publications

References 9 publications

The utility of human fallopian tube mucosa as a novel source of multipotent stem cells for the treatment of autologous reproductive tract injury

The utility of human fallopian tube mucosa as a novel source of multipotent stem cells for the treatment of autologous reproductive tract injury

Review of transvaginal natural orifice transluminal endoscopic surgery in gynecology

Per-oral endoscopic salpingo-oophorectomy (POESY) in humans: a prospective clinical feasibility study

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