Effect of cultivars, Fusarium strains and storage temperature on trichothecenes production in inoculated potato tubers

Xue, Huali; Bi, Yang; Tang, Yamei; Zhao, Ying; Wang, Yi

doi:10.1016/j.foodchem.2013.11.060

Cited by 30 publications

(25 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results in the current study are confirmed by the findings of [19]. Who reported that the Fusarium solani had the strongest ability to produce T-2 toxin in both susceptible and resistant cultivars.…”

Section: Molecular Analysissupporting

confidence: 89%

“…T1 T2 T3 T4 T5 T6 T7 T8 T9 T10 T11 T12 T13 fresh root weight (g) T1 T2 T3 T4 T5 T6 T7 T8 T9 T10 T11 T12 T13 dry root (g) weight The results of this study are in agreement with those of previous literatures [18] who the first molecular identification of Fusarium solani f. sp. cucurbite race1 and race 2 by using PCR assay [19] reported that the primer pair Fsc1 amplified 580 bp fragments from genome DNA of the three race 1 isolates Fsolani f. sp. cucurbitae none of the race 2 isolates including generated the specific amplified fragment…”

Section: Molecular Analysismentioning

confidence: 99%

See 1 more Smart Citation

Genetic Diversity of Fusarium solani f. sp. cucurbitae, the Causal Root and Crown Rot of Cucurbits (Melon) by Using Molecular Markers and Control

Abdul-Hasan¹,

Hussein²

2016

AJPS

View full text Add to dashboard Cite

Detection of F. solani f. sp. cucurbitae causal agent of the crown and root rot disease of melon race 1, race 2 is difficult. It is based only on morphological characteristic. In this study, forty isolates identified as Fusarium solani based on morphological characterization, F. solani was one of the most frequently isolated species. Molecular identification of these isolates by PCR technique using species-specific primer, indicated that thirty-two isolates, amplified product 580 bp (race 1) and two isolate amplified product 580 bp (race 2), while six isolates were not amplified with primer of both races. Production of Trichothecenes (T2-toxen, DON.) by Fusarium solani was conducted on isolates confirmed as belonging in the F. solani by PCR. The results indicated that the presence of Tri5, Tri13 genes is coding the ability of synthesis mycotoxin. In vitro, the results indicated that NPs (AgNPs, MgNPs) and chemical (Phylex) possess the antifungal properties against at various level. Treatment with (AgNPs 150 ppm, MgNPs 2%, 3% ppm) and 3% Phylex resulted in maximum inhabitation of F. solani. In vivo, five characters (height plant, hoot ant root fresh and dry weight) were measured based on the greenhouse, field experimental results. Treatment with (AgNPs, MgNPs) and Phylex had higher measured parameters than positive control.

show abstract

Section: Molecular Analysissupporting

confidence: 89%

Section: Molecular Analysismentioning

confidence: 99%

Genetic Diversity of Fusarium solani f. sp. cucurbitae, the Causal Root and Crown Rot of Cucurbits (Melon) by Using Molecular Markers and Control

Abdul-Hasan¹,

Hussein²

2016

AJPS

View full text Add to dashboard Cite

show abstract

“…Many of these mutations may be not detected by simple PCR markers. Moreover, the production of mycotoxins may change depending on environmental conditions (Mateo et al 2002) and the susceptibility of a given potato cultivar (Xue et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Diversity of Fusarium spp. associated with dry rot of potato tubers in Poland

et al. 2016

View full text Add to dashboard Cite

Fusarium spp. belong to the division Ascomycota and cause important plant diseases; these fungi may contaminate food products with mycotoxins, endangering human and animal health. Several Fusarium spp. have been associated with potato dry rot. The most frequent and devastating of these species are F. sambucinum, F. solani and F. oxysporum, depending on the geographic location and the season. Samples of potato tubers with dry rot symptoms were collected, and their putative fungal isolates were identified as Fusarium species using partial nucleotide sequences of the internal transcribed spacer, translation elongation factor 1-α and β-tubulin genes. Among 149 isolates, 12 species were identified. F. oxysporum was the most frequent (45 % of the isolates), followed by F. avenaceum (12.1 %), F. solani (10.7 %) and F. sambucinum (7.4 %). Phylogenetic analyses confirmed the species identifications and revealed a high diversity of F. solani and a low diversity of F. oxysporum. Potential producers of zearalenone and trichothecenes were identified within the obtained isolates using PCR markers. Isolates that were pathogenic to potatoes in laboratory tests were found in four species: F. sambucinum, F. avenaceum, F. culmorum, and F. graminearum. The effects of increased temperature and mixed inoculum on the pathogenicities of chosen species were evaluated. This study adds 434 potato-derived Fusarium sequences to the NCBI GenBank database and demonstrates that the list of Fusarium species and mycotoxins present in potato tubers may be richer than previously believed, regardless of whether these species cause dry rot or live as saprophytes.

show abstract

“…Types A and B are commonly found in grain, animal feed and human food produced from contaminated grain (Sudakin 2003;Jin et al 2010) as well as traditional Chinese medicines ). In addition, these mycotoxins have also been detected in tomato fruit (Harwig et al 1979), banana (Chakrabarti & Ghosal 1986), and potato tubers (Desjardins & Plattner 1989;Xue et al 2014). China is one of the largest apple producers in the world.…”

Section: Introductionmentioning

confidence: 99%

“…However, most of these methods were developed for cereal grain such as wheat, barley, oat, maize, rice and their derived products (i.e. bread, malt and beer) (Ibáñez-Vea et al 2011), and potato tubers (Xue et al 2014). No reports were found for the determination of trichothecenes in core rot in the pulp tissue of apple fruit.…”

Section: Introductionmentioning

confidence: 99%

A method of analysis for T-2 toxin and neosolaniol by UPLC-MS/MS in apple fruit inoculated withTrichothecium roseum

Tang¹,

Xue²,

Bi³

et al. 2014

Food Additives & Contaminants: Part A

Self Cite

View full text Add to dashboard Cite

Trichothecenes are one of the most important groups of mycotoxins produced by Trichothecium roseum, which causes core rot of apple. A reliable and sensitive method was developed and successfully applied for the rapid detection of trichothecenes including T-2 toxin and neosolaniol in harvested apple using UPLC-MS/MS. After the extraction of the two mycotoxins from the apple matrix with methanol/water (80/20, v/v), the concentrated extracts were cleaned-up by PriboFast M270 columns and then analysed by UPLC-MS/MS. T-2 toxin and neosolaniol were effectively separated as unique peaks. The validity of this method was established by its linearity (R(2) ≥ 0.9995), precision (relative standard deviation ≤ 3.6%), accuracy, selectivity, limit of detection of 2-5 μg kg(-1), limit of quantification of 5-10 μg kg(-1) and average recovery of 73-96%. Levels of T-2 toxin were found in the range 7.1-128.4 µg kg(-1) in the core rot lesion of three cultivars apple (cvs. Red Delicious, Fuji and Ralls). T-2 was detected not only in the lesion, but also in the tissue without any disease symptoms. However, neosolaniol was only detected in the lesion on 'Red Delicious' apples. In addition, the concentration of T-2 toxin in the susceptible cultivar (cv. Fuji) was significantly higher than that in the resistant one (cv. Ralls). This method proved to be suitable at detecting T-2 and neosolaniol simultaneously in apples infected with T. roseum.

show abstract

Effect of cultivars, Fusarium strains and storage temperature on trichothecenes production in inoculated potato tubers

Cited by 30 publications

References 21 publications

Genetic Diversity of <i>Fusarium solani</i> f. sp. <i>cucurbitae</i>, the Causal Root and Crown Rot of Cucurbits (Melon) by Using Molecular Markers and Control

Genetic Diversity of <i>Fusarium solani</i> f. sp. <i>cucurbitae</i>, the Causal Root and Crown Rot of Cucurbits (Melon) by Using Molecular Markers and Control

Diversity of Fusarium spp. associated with dry rot of potato tubers in Poland

A method of analysis for T-2 toxin and neosolaniol by UPLC-MS/MS in apple fruit inoculated withTrichothecium roseum

Contact Info

Product

Resources

About

Effect of cultivars, Fusarium strains and storage temperature on trichothecenes production in inoculated potato tubers

Cited by 30 publications

References 21 publications

Genetic Diversity of &lt;i&gt;Fusarium solani&lt;/i&gt; f. sp. &lt;i&gt;cucurbitae&lt;/i&gt;, the Causal Root and Crown Rot of Cucurbits (Melon) by Using Molecular Markers and Control

Genetic Diversity of &lt;i&gt;Fusarium solani&lt;/i&gt; f. sp. &lt;i&gt;cucurbitae&lt;/i&gt;, the Causal Root and Crown Rot of Cucurbits (Melon) by Using Molecular Markers and Control

Diversity of Fusarium spp. associated with dry rot of potato tubers in Poland

A method of analysis for T-2 toxin and neosolaniol by UPLC-MS/MS in apple fruit inoculated withTrichothecium roseum

Contact Info

Product

Resources

About

Genetic Diversity of <i>Fusarium solani</i> f. sp. <i>cucurbitae</i>, the Causal Root and Crown Rot of Cucurbits (Melon) by Using Molecular Markers and Control

Genetic Diversity of <i>Fusarium solani</i> f. sp. <i>cucurbitae</i>, the Causal Root and Crown Rot of Cucurbits (Melon) by Using Molecular Markers and Control