FAD binding properties of a cytosolic version of Escherichia coli NADH dehydrogenase-2

Nanoenvironmental effect in AOT reverse micelles on the triplet excited state properties of flavins and quenching by molecular oxygen

“…Steady‐state and time‐resolved techniques used in this work were fully described in previous reports …”

Enhancement of Photophysical and Photosensitizing Properties of Flavin Adenine Dinucleotide by Mutagenesis of the C‐Terminal Extension of a Bacterial Flavodoxin Reductase

“…In addition, the excitation beam was attenuated with a neutral density filter with approximately 20 % of transmittance to avoid photochemical processes during acquisition. The intrinsic and cofactor fluorescence quantum yields ( Φ F ) of the enzymes in air‐saturated buffer solutions were determined by comparison of the integrated fluorescence intensity using the emission spectra of authentic solutions of Trp (${\Phi _{\rm{F}}^{{\rm{Trp}}} }$ =0.14)12a and FAD (${\Phi _{\rm{F}}^{{\rm{FAD}}} }$ =0.028)38 in the same buffer as reference, with matched absorbance ( A ≈0.05) at the excitation wavelength to minimize internal filter effects. The steady‐state fluorescence anisotropy r of the samples was determined with a classical L ‐format and calculated as previously described 38…”

Effect of dye localization and self-interactions on the photosensitized generation of singlet oxygen by rose bengal bound to bovine serum albumin

“…Since RB absorbs light both at the excitation and emission spectral regions of BSA, the observed emission spectra were corrected by the primary and secondary inner filter effects as described before [20,21].…”

“…The fluorescence quantum yield (U F ) of RB in BSA solutions was determined by comparison of the integrated fluorescence intensity using as reference the emission of dye in buffer solution (U F = 0.018) [19]; keeping constant the absorbance value at the excitation wavelength (525 nm). Steadystate fluorescence anisotropy r of the RB was determined with a classical L-format and calculated as described elsewhere [20,21].…”