Differential transcriptional profile of Corynebacterium pseudotuberculosis in response to abiotic stresses

Abstract

BackgroundThe completion of whole-genome sequencing for Corynebacterium pseudotuberculosis strain 1002 has contributed to major advances in research aimed at understanding the biology of this microorganism. This bacterium causes significant loss to goat and sheep farmers because it is the causal agent of the infectious disease caseous lymphadenitis, which may lead to outcomes ranging from skin injury to animal death. In the current study, we simulated the conditions experienced by the bacteria during host infection. By sequencing transcripts using the SOLiDTM 3 Plus platform, we identified new targets expected to potentiate the survival and replication of the pathogen in adverse environments. These results may also identify possible candidates useful for the development of vaccines, diagnostic kits or therapies aimed at the reduction of losses in agribusiness.ResultsUnder the 3 simulated conditions (acid, osmotic and thermal shock stresses), 474 differentially expressed genes exhibiting at least a 2-fold change in expression levels were identified. Important genes to the infection process were induced, such as those involved in virulence, defence against oxidative stress, adhesion and regulation, and many genes encoded hypothetical proteins, indicating that further investigation of the bacterium is necessary. The data will contribute to a better understanding of the biology of C. pseudotuberculosis and to studies investigating strategies to control the disease.ConclusionsDespite the veterinary importance of C. pseudotuberculosis, the bacterium is poorly characterised; therefore, effective treatments for caseous lymphadenitis have been difficult to establish. Through the use of RNAseq, these results provide a better biological understanding of this bacterium, shed light on the most likely survival mechanisms used by this microorganism in adverse environments and identify candidates that may help reduce or even eradicate the problems caused by this disease.

Co-localization of major quantitative trait loci for pod size and weight to a 3.7 cM interval on chromosome A05 in cultivated peanut (Arachis hypogaea L.)

“…The linkage groups (LGs) were designated as chromosome A01-A10 and B01-B10 based on the common markers as a previously published integrated consensus map [43]. This consensus map was constructed based on 16 genetic linkage maps [43] and used as reference in other publications [3, 15, 44]. The graphical presentation of genetic linkage map was generated with the MapChart 2.3 software [45].…”

Development of SSR markers and identification of major quantitative trait loci controlling shelling percentage in cultivated peanut (Arachis hypogaea L.)

“…The linkage groups (LGs) were designated as A1–A10 and B1–B10 by aligning the markers to the integrated consensus genetic map (Shirasawa et al 2013) and the genome sequences of A. duranensis and Arachis ipaensis (Bertioli et al 2016). This consensus genetic map was integrated based on 16 genetic maps and used as reference in other publications (Chen et al 2016; Huang et al 2015; Zhou et al 2014). …”

In silico identification of essential proteins in Corynebacterium pseudotuberculosis based on protein-protein interaction networks

“…KatA is an oxidoreductase enzyme which decomposes hydrogen peroxide (H 2 O 2 ). It was already studied for instance in C. glutamicum [68, 69] and C. pseudotuberculosis [70]. Endonuclease III (Nth) has a homologous counterpart in Haemophilus influenzae stored in DEG.…”