Application of a modified EDTA-mediated exudation technique and guttation fluid analysis for Potato spindle tuber viroid RNA detection in tomato plants (Solanum lycopersicum)

Kovalskaya, Natalia; Owens, Robert A.; Baker, C. Jacyn; Deahl, Kenneth L.; Hammond, Rosemarie W.

doi:10.1016/j.jviromet.2013.12.015

Cited by 10 publications

(4 citation statements)

References 66 publications

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“…Thus, we measured amino acid concentrations in different plant species by EDTA-exudation, a method preventing sieve element sealing and allowing us to get enough volume to measure amino acids. It has been adopted in many studies 16 20 21 22 23 24 25 26 27 and our results indicated that it was reliable in measuring amino acid concentrations with a relatively low variation among biological replicates ( Fig. 2b ).…”

Section: Discussionmentioning

confidence: 75%

Nitrogen hurdle of host alternation for a polyphagous aphid and the associated changes of endosymbionts

Liu

Kang

Guo

et al. 2016

Sci Rep

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Low proportion of essential amino acids (EAAs) is one of the barriers for animals to use phloem as a diet. Endosymbionts with EAAs synthesis functions are considered crucial for ameliorating the lack of EAAs in insects’ diets. In this study, we transferred the insects from a cabbage-reared Myzus persicae population onto 3 new plant species including eggplant, tobacco and spinach. The performance on these plants was evaluated and the dynamics of endosymbionts in relation to this host alternation were recorded. We found that the EAAs ratio in phloem was largely determined by the concentrations of non-essential amino acids and the higher proportion of EAAs seemed to favor the population establishment on new plant species and the growth of primary endosymbionts inside insects, which indicated that nitrogen quality was an important factor for aphids to infest and spread on new plant hosts.

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Section: Discussionmentioning

confidence: 75%

Nitrogen hurdle of host alternation for a polyphagous aphid and the associated changes of endosymbionts

Liu

Kang

Guo

et al. 2016

Sci Rep

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“…Both methods lead to either highly diluted phloem sap or very low sample amounts. EDTA-facilitated exudation is also prone to contamination and might not represent the real phloem composition 16 . Spontaneous phloem exudation is limited to plant species like cucurbits, lupine or yucca, where cutting off plant parts leads to a spontaneous emission of phloem sap that can be easily collected 13 17 18 19 .…”

Section: Introductionmentioning

confidence: 99%

Phloem Sap Sampling from <em>Brassica napus</em> for 3D-PAGE of Protein and Ribonucleoprotein Complexes

Pahlow¹,

Ostendorp²,

Krüßel³

et al. 2018

JoVE

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Sampling the phloem of higher plants is often laborious and significantly dependent on the plant species. However, proteome studies under denaturing conditions could be achieved in different plant species. Native protein:protein and protein:nucleic acid complexes from phloem samples have as yet scarcely been analyzed, although they might play important roles in maintenance of this specialized compartment or in long-distance signaling. Large molecular assemblies can be isolated using a blue native gel electrophoresis (BN-PAGE). Their protein components can be separated by a subsequent sodium dodecyl sulfate PAGE (SDS-PAGE). However, proteins with similar molecular weights co-migrate, what can hinder protein identification by mass spectrometry. Combining BN-PAGE with two different denaturing gel electrophoresis steps, namely Tris-Tricine-urea and SDS-PAGE, enables the additional separation of proteins according to their hydrophilicity/hydrophobicity and thus increases resolution and the success of protein identification. It even allows distinguishing proteins that only differ in their posttranslational modifications. In addition, blue native northern blotting can be applied to identify the RNA components in macromolecular complexes. We show that our protocol is suitable to unravel the protein and RNA components of native protein:protein and ribonucleoprotein (RNP) complexes occurring in phloem samples. Combining a blue native PAGE with two different denaturing PAGE steps can help to separate different kinds of large protein complexes, and also enables an increased identification rate of their components by mass spectrometry. Furthermore, the protocol is robust enough to simultaneously detect potentially bound nucleic acids within single protein complexes.

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“…Guttation is a very common natural phenomenon in the plant kingdom. Many plant species reportedly engage in guttation, such as rice, wheat, barley, maize, tomato, potatoes, tobacco [1][2][3][4][5]. Guttation refers to the process of liquid seeping from the tips, edges, and adaxial and abaxial surfaces of the undamaged leaves of plant species in the form of droplets [6].…”

Section: Introductionmentioning

confidence: 99%

The Effect of Guttation on the Growth of Bamboo Shoots

Zheng

Cai

Bai

et al. 2021

Forests

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Guttation is the process of exudating droplets from the tips, edges, and adaxial and abaxial surfaces of the undamaged leaves. Guttation is a natural and spontaneous biological phenomenon that occurs in a wide variety of plants. Despite its generally positive effect on plant growth, many aspects of this cryptic process are unknown. In this study, the guttation phenomenon characteristic of bamboo shoots and the anatomical feature of these and culm sheaths were systematically observed. In addition, the water transport pathway and the compounds in guttation droplets of bamboo shoots were analyzed, and the effect of bamboo sheaths’ guttation on the growth of bamboo shoots was assessed. The results revealed that bamboo shoots began to exudate liquid in the evening through to the next morning, during which period the volume of guttation liquid gradually increases and then decreases before sunrise. Many vascular bundles are in bamboo shoots and culm sheaths to facilitate this water transport. The exudate liquid contains organic acids, sugars, and hormones, among other compounds. Our findings suggest that the regular guttation of the sheath blade is crucial to maintain the normal growth of bamboo shoots.

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Application of a modified EDTA-mediated exudation technique and guttation fluid analysis for Potato spindle tuber viroid RNA detection in tomato plants (Solanum lycopersicum)

Cited by 10 publications

References 66 publications

Nitrogen hurdle of host alternation for a polyphagous aphid and the associated changes of endosymbionts

Nitrogen hurdle of host alternation for a polyphagous aphid and the associated changes of endosymbionts

Phloem Sap Sampling from <em>Brassica napus</em> for 3D-PAGE of Protein and Ribonucleoprotein Complexes

The Effect of Guttation on the Growth of Bamboo Shoots

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