Control of the Development and Prevalence of Antimicrobial Resistance in Bacteria of Food Animal Origin in Japan: A New Approach for Risk Management of Antimicrobial Veterinary Medicinal Products in Japan

Asai, Tetsuo; Hiki, Mototaka; Ozawa, Manao; Koike, Ryuji; Eguchi, Kaoru; Kawanishi, Michihiro; Kojima, Akira; Endoh, Y. S.; Hamamoto, Shuichi; Sakai, Masato; Sekiya, Tatsuro

doi:10.1089/fpd.2013.1649

Cited by 14 publications

(11 citation statements)

References 4 publications

(4 reference statements)

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“…Higher phenotypic resistance in Brucella against these antimicrobials is likely to limits the treatment effectiveness, owing to the increased number of infections. Emergence of multidrug resistance Brucella in livestock species in this study may pose serious threat to humans as these bacteria often transferred from animals to humans through food chain [69]. Being a zoonotic pathogen and given the emergence of increased antimicrobial resistance in Brucella species, the situation with respect to hospital care may worsen and limits the treatment options in public health settings.…”

Section: Discussionmentioning

confidence: 95%

Identification, Genotyping and Antimicrobial Susceptibility Testing of Brucella spp. Isolated from Livestock in Egypt

et al. 2019

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Brucellosis is a highly contagious zoonosis worldwide with economic and public health impacts. The aim of the present study was to identify Brucella (B.) spp. isolated from animal populations located in different districts of Egypt and to determine their antimicrobial resistance. In total, 34-suspected Brucella isolates were recovered from lymph nodes, milk, and fetal abomasal contents of infected cattle, buffaloes, sheep, and goats from nine districts in Egypt. The isolates were identified by microbiological methods and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Differentiation and genotyping were confirmed using multiplex PCR for B. abortus, Brucella melitensis, Brucella ovis, and Brucella suis (AMOS) and Bruce-ladder PCR. Antimicrobial susceptibility testing against clinically used antimicrobial agents (chloramphenicol, ciprofloxacin, erythromycin, gentamicin, imipenem, rifampicin, streptomycin, and tetracycline) was performed using E-Test. The antimicrobial resistance-associated genes and mutations in Brucella isolates were confirmed using molecular tools. In total, 29 Brucella isolates (eight B. abortus biovar 1 and 21 B. melitensis biovar 3) were identified and typed. The resistance of B. melitensis to ciprofloxacin, erythromycin, imipenem, rifampicin, and streptomycin were 76.2%, 19.0%, 76.2%, 66.7%, and 4.8%, respectively. Whereas, 25.0%, 87.5%, 25.0%, and 37.5% of B. abortus were resistant to ciprofloxacin, erythromycin, imipenem, and rifampicin, respectively. Mutations in the rpoB gene associated with rifampicin resistance were identified in all phenotypically resistant isolates. Mutations in gyrA and gyrB genes associated with ciprofloxacin resistance were identified in four phenotypically resistant isolates of B. melitensis. This is the first study highlighting the antimicrobial resistance in Brucella isolated from different animal species in Egypt. Mutations detected in genes associated with antimicrobial resistance unravel the molecular mechanisms of resistance in Brucella isolates from Egypt. The mutations in the rpoB gene in phenotypically resistant B. abortus isolates in this study were reported for the first time in Egypt.

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Section: Discussionmentioning

confidence: 95%

Identification, Genotyping and Antimicrobial Susceptibility Testing of Brucella spp. Isolated from Livestock in Egypt

et al. 2019

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“…The use of these antibiotics for the promotion of growth, generally at low doses, is a risk factor for bacterial resistance [17]. The resistant strains found in animal productions can be transmitted to humans through the food chain and compromise the efficacy of antimicrobials in human medicine [69]. Therefore, the consideration of agricultural and livestock sectors in the prevention and control of bacterial resistance is essential.…”

Section: Discussionmentioning

confidence: 99%

Assessment of General Public’s Knowledge and Opinions towards Antibiotic Use and Bacterial Resistance: A Cross-Sectional Study in an Urban Setting, Rufisque, Senegal

et al. 2018

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Background: Bacterial resistance is a major public health problem worldwide. One solution to this scourge is to sensitize the general public on rational use of antibiotics. Our goal was to assess people’s knowledge and opinions about antibiotic use and bacterial resistance in an urban setting. Method: We performed a cross-sectional study. A convenience sampling was done. A questionnaire was administered to 400 persons during face-to-face interviews. Results: Most respondents thought that antibiotics are effective against colds/flu (69.8%), cough (72.3%) and sore throat (64.4%). At the same time, 42.8% stated that antibiotic therapy can be stopped as soon as the symptoms disappear. Only 8.8% and 41.8% of people knew that handwashing and vaccination prevented bacterial resistance. Globally, 7% of people had a good knowledge. Socio-demographic variables were not associated with the level of knowledge. The main sources of information were entourage and pharmacy staff. Regarding the opinions, 78.3% of surveyed participants the people thought that that people overuse antibiotics. Additionally, 28% said that they have no role to play against bacterial resistance. Conclusion: People living in an urban setting had a low knowledge about antibiotic use and bacterial resistance. There is a need to implement awareness campaigns. Further studies on population practices toward antibiotic use are necessary.

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“…However, the indiscriminate use of antimicrobials can result in bacterial selection pressure of the intestinal microbiota of animals [ 1 – 3 ]. Because multiresistant bacteria are frequently found in poultry meat [ 4 – 6 ], chicken products are suspected to be a source of foodborne pathogen and/or antimicrobial resistance bacteria for humans [ 1 – 3 , 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

Comparison of Antibiotic Resistance and Virulence Factors amongEscherichia coliIsolated from Conventional and Free-Range Poultry

Koga

Scandorieiro

Vespero

et al. 2015

BioMed Research International

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Microbiological contamination in commercial poultry production has caused concerns for human health because of both the presence of pathogenic microorganisms and the increase in antimicrobial resistance in bacterial strains that can cause treatment failure of human infections. The aim of our study was to analyze the profile of antimicrobial resistance and virulence factors of E. coli isolates from chicken carcasses obtained from different farming systems (conventional and free-range poultry). A total of 156 E. coli strains were isolated and characterized for genes encoding virulence factors described in extraintestinal pathogenic E. coli (ExPEC). Antimicrobial susceptibility testing was performed for 15 antimicrobials, and strains were confirmed as extended spectrum of β-lactamases- (ESBLs-) producing E. coli by phenotypic and genotypic tests. The results indicated that strains from free-range poultry have fewer virulence factors than strains from conventional poultry. Strains from conventionally raised chickens had a higher frequency of antimicrobial resistance for all antibiotics tested and also exhibited genes encoding ESBL and AmpC, unlike free-range poultry isolates, which did not. Group 2 CTX-M and CIT were the most prevalent ESBL and AmpC genes, respectively. The farming systems of poultries can be related with the frequency of virulence factors and resistance to antimicrobials in bacteria.

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Control of the Development and Prevalence of Antimicrobial Resistance in Bacteria of Food Animal Origin in Japan: A New Approach for Risk Management of Antimicrobial Veterinary Medicinal Products in Japan

Cited by 14 publications

References 4 publications

Identification, Genotyping and Antimicrobial Susceptibility Testing of Brucella spp. Isolated from Livestock in Egypt

Identification, Genotyping and Antimicrobial Susceptibility Testing of Brucella spp. Isolated from Livestock in Egypt

Assessment of General Public’s Knowledge and Opinions towards Antibiotic Use and Bacterial Resistance: A Cross-Sectional Study in an Urban Setting, Rufisque, Senegal

Comparison of Antibiotic Resistance and Virulence Factors amongEscherichia coliIsolated from Conventional and Free-Range Poultry

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