Anti‐inflammatory Properties of Gastric‐derived <i>Lactobacillus plantarum </i><scp>XB</scp>7 in the Context of <i>Helicobacter pylori</i> Infection

Thiraworawong, Thien; Spinler, Jennifer K.; Werawatganon, Duangporn; Klaikeaw, Naruemon; Venable, Susan; Versalovic, James; Tumwasorn, Somying

doi:10.1111/hel.12105

Cited by 30 publications

(39 citation statements)

References 63 publications

(75 reference statements)

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“…On the taxonomic level, the Lactobacillales-toLactobacillus lineage was decreased in Vdr À/À fecal stool samples, correlating VDR with bacteria that produce lactic acid, which benefits the intestine by lowering the pH and balancing the microflora 26 and also by its anti-inflammatory [27][28][29] and antitumor features. [30][31][32] In Vdr À/À fecal stool, the increased levels of Bacteroides may predispose the mice to colon cancer, 33 consistent with the observation that Vdr À/À mice are more prone to colonic tumorigenesis.…”

Section: Discussionmentioning

confidence: 99%

Lack of Vitamin D Receptor Causes Dysbiosis and Changes the Functions of the Murine Intestinal Microbiome

Jin

Zhang

et al. 2015

Clinical Therapeutics

198

142

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Section: Discussionmentioning

confidence: 99%

Lack of Vitamin D Receptor Causes Dysbiosis and Changes the Functions of the Murine Intestinal Microbiome

Jin

Zhang

et al. 2015

Clinical Therapeutics

198

142

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“…However, pro-or anti-inflammation for the host immune system might occur on a case by case basis upon use of different probiotic strains (Turroni et al, 2014). Previous studies have reported an anti-inflammatory property of Lactobacillus plantarum in several animal disease models (Liu et al, 2011;Thiraworawong et al, 2014). Studies in the mouse colitis model demonstrated that an anti-inflammatory cytokine of IL-10 was up- regulated by Lactobacillus plantarum supplementation (Duary et al, 2012); however, no significant increase in IL-10 was found in L. plantarum ZDY 2013 pretreated mice, suggesting that other factors rather than IL-10 were involved in the anti-inflammatory activity of L. plantarum ZDY 2013.…”

Section: Discussionmentioning

confidence: 99%

Changes in gastric microbiota induced by Helicobacter pylori infection and preventive effects of Lactobacillus plantarum ZDY 2013 against such infection

Pan

Wan

Xie

et al. 2016

Journal of Dairy Science

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Helicobacter pylori is a gram-negative pathogen linked to gastric ulcers and stomach cancer. Gastric microbiota might play an essential role in the pathogenesis of these stomach diseases. In this study, we investigated the preventive effect of a probiotic candidate Lactobacillus plantarum ZDY 2013 as a protective agent against the gastric mucosal inflammation and alteration of gastric microbiota induced by H. pylori infection in a mouse model. Prior to infection, mice were pretreated with or without 400 µL of L. plantarum ZDY 2013 at a concentration of 10(9) cfu/mL per mouse. At 6 wk postinfection, gastric mucosal immune response and alteration in gastric microbiota mice were examined by quantitative real-time PCR and high-throughput 16S rRNA gene amplicon sequencing, respectively. The results showed that L. plantarum ZDY 2013 pretreatment prevented increase in inflammatory cytokines (e.g., IL-1β and IFN-γ) and inflammatory cell infiltration in gastric lamina propria induced by H. pylori infection. Weighted UniFrac principal coordinate analysis showed that L. plantarum ZDY 2013 pretreatment prevented the alteration in gastric microbiota post-H. pylori infection. Linear discriminant analysis coupled with effect size identified 22 bacterial taxa (e.g., Pasteurellaceae, Erysipelotrichaceae, Halomonadaceae, Helicobacteraceae, and Spirochaetaceae) that overgrew in the gastric microbiota of H. pylori-infected mice, and most of them belonged to the Proteobacteria phylum. Lactobacillus plantarum ZDY 2013 pretreatment prevented this alteration; only 6 taxa (e.g., Lachnospiraceae, Ruminococcaceae, and Clostridiaceae), mainly from the taxa of Firmicutes and Bacteroidetes, were dominant in the gastric microbiota of the L. plantarum ZDY 2013 pretreated mice. Administration of L. plantarum ZDY 2013 for 3 wk led to increase in several bacterial taxa (e.g., Rikenella, Staphylococcus, Bifidobacterium), although a nonsignificant alteration was found in the gastric microbiota. Overall, this study demonstrated that L. plantarum ZDY 2013 pretreatment played an important role in preventing gastric mucosal inflammation and gastric microbiota alteration induced by H. pylori infection, and the selective modulation in gastric microbiota posed by this intervention suggested that targeting gastric microbiota through oral administration of probiotics might be an alternative strategy to prevent H. pylori infection.

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“…MKN45 cells were washed with PBS twice and suspended in TRIzol reagent (Invitrogen, Carlsbad, CA, USA) to isolate RNA, according to the manufacturer's instructions. Then, the isolated RNA was transcribed into cDNA using a ReverTra Ace a kit (Toyobo Co., Ltd., Osaka, Japan) and an oligo-dT (20) primer according to the manufacturer's instructions. PCR amplifications of CXCL8 and CCL5 were performed using TaqMan Gene Expression Master Mix (Applied Biosystems) with CXCL8-specific primers and probes (TaqMan Gene Expression Assays Hs00174103_m1, Applied Biosystems) and CCL5-specific primers and probes (TaqMan Gene Expression Assays Hs00174575_m1, Applied Biosystems), following the manufacturer's protocol.…”

Section: Analysis Of Gene Expression In Mkn45 Cellsmentioning

confidence: 99%

“…Moreover, probiotics have the potential to diminish side effects of antibiotics, increase the H. pylori eradication rate, and decrease host cell damage [10,16]. Some probiotic LAB strains have been reported to suppress IL-8 production in response to H. pylori, both in vitro and in vivo [17][18][19][20]. Additionally, a few studies have investigated the mechanisms underlying the suppression of H. pylori-induced IL-8 by probiotics.…”

Section: Introductionmentioning

confidence: 99%

Lactobacillus paracasei strain 06TCa19 suppresses inflammatory chemokine induced by Helicobacter pylori in human gastric epithelial cells

et al. 2017

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Helicobacter (H.) pylori infection is an important risk factor for gastric cancer that causes gastric inflammation. Inflammatory chemokines such as interleukin (IL)-8 and regulated on activation normal T cell expressed and secreted (RANTES) are elevated in the gastric mucosa by H. pylori. This study aimed to investigate the effects of Lactobacillus paracasei strain 06TCa19, a probiotic strain, on IL-8 and RANTES expression and production induced by H. pylori using human gastric epithelial cell lines. Strain 06TCa19 was shown to suppress H. pylori-mediated elevation of gene expression related to these chemokines in MKN45 cells. The strain also suppressed the increase in IL-8 and RANTES products induced by H. pylori in AGS cells as well as in MKN45 cells. In MKN45 cells inoculated with H. pylori, strain 06TCa19 was shown to downregulate the activation of NF-κB and p38 MAPK signaling pathways. Additionally, the level of the CagA virulence protein of H. pylori in the MKN45 cells and the number of viable H. pylori adhering to MKN45 cells decreased with the addition of strain 06TCa19. Moreover, the strain 06TCa19 notably increased lactic acid in the supernatant of MKN45 cells. Thus, lactic acid released from strain 06TCa19 might have inhibited the adhesion of H. pylori to MKN45 cells and prevented the insertion of H. pylori CagA into the cells, and elevation of IL-8 and RANTES genes and proteins might be suppressed by downregulating the NF-κB and p38 MAPK pathways. Therefore, use of strain 06TCa19 may prevent H. pylori-associated gastric inflammation.

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Anti‐inflammatory Properties of Gastric‐derived Lactobacillus plantarum XB7 in the Context of Helicobacter pylori Infection

Abstract: These results suggest that L. plantarum XB7 produces secreted factors capable of modulating inflammation during H. pylori infection, and this probiotic Lactobacillus strain shows promise as an adjunctive therapy for treating H. pylori-associated disease.

Cited by 30 publications

References 63 publications

Lack of Vitamin D Receptor Causes Dysbiosis and Changes the Functions of the Murine Intestinal Microbiome

Lack of Vitamin D Receptor Causes Dysbiosis and Changes the Functions of the Murine Intestinal Microbiome

Changes in gastric microbiota induced by Helicobacter pylori infection and preventive effects of Lactobacillus plantarum ZDY 2013 against such infection

Lactobacillus paracasei strain 06TCa19 suppresses inflammatory chemokine induced by Helicobacter pylori in human gastric epithelial cells

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