Characterization and expression analysis of WOX5 genes from wheat and its relatives

Zhao, Shaofan; Jiang, Qiantao; Ma, Jason; Zhang, Xiaowei; Zhao, Quanzhi; Wang, Xiuying; Wang, Changshui; Cao, Xue; Lu, Zhen-Xiang; Zheng, Yu; Wei, Yuming

doi:10.1016/j.gene.2013.12.022

Cited by 30 publications

(18 citation statements)

References 36 publications

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“…AtWOX5 and AtWOX7 are reportedly involved in the development of Arabidopsis roots [12,25]. A tissue expression analysis also showed that TaWOX9 is mainly expressed in wheat roots, which is similar to previous research [26].…”

Section: Putative Functions Of Tawox Genes Based On Sequence Homologysupporting

confidence: 87%

Identification of the WUSCHEL-Related Homeobox (WOX) Gene Family, and Interaction and Functional Analysis of TaWOX9 and TaWUS in Wheat

Liú

Xia

et al. 2020

IJMS

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The WUSCHEL-related homeobox (WOX) is a family of plant-specific transcription factors, with important functions, such as regulating the dynamic balance of division and differentiation of plant stem cells and plant organ development. We identified 14 distinct TaWOX genes in the wheat (Triticum aestivum L.) genome, based on a genome-wide scan approach. All of the genes under evaluation had positional homoeologs on subgenomes A, B and D except TaWUS and TaWOX14. Both TaWOX14a and TaWOX14d had a paralogous copy on the same genome due to tandem duplication events. A phylogenetic analysis revealed that TaWOX genes could be divided into three groups. We performed functional characterization of TaWOX genes based on the evolutionary relationships among the WOX gene families of wheat, rice (Oryza sativa L.), and Arabidopsis. An overexpression analysis of TaWUS in Arabidopsis revealed that it affected the development of outer floral whorl organs. The overexpression analysis of TaWOX9 in Arabidopsis revealed that it promoted the root development. In addition, we identified some interaction between the TaWUS and TaWOX9 proteins by screening wheat cDNA expression libraries, which informed directions for further research to determine the functions of TaWUS and TaWOX9. This study represents the first comprehensive data on members of the WOX gene family in wheat.

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Section: Putative Functions Of Tawox Genes Based On Sequence Homologysupporting

confidence: 87%

Identification of the WUSCHEL-Related Homeobox (WOX) Gene Family, and Interaction and Functional Analysis of TaWOX9 and TaWUS in Wheat

Liú

Xia

et al. 2020

IJMS

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“…The structure and functions of WOX5 TF in cereals and dicotyledonous do not have serious differences; the expression of WOX5 orthologs in cereals is concentrated in the RAM OC; it also takes place in zygotic and somatic embryogenesis, and it is regulated by a CLAVATA like system, auxins, and cytokinins [109,110].…”

Section: The Main Trends In Evolution Of Wox Tfsmentioning

confidence: 99%

Transcription factors in developmental genetics and the evolution of higher plants

et al. 2015

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Transcription factors play an essential role in controlling various developmental programs in plants, coordinating the action of any genetic network. Among the most important groups of plant transcrip tion factors are the homeodomain containing transcription factors, in particular, those belonging to the KNOX and WOX families, the functions of which are associated with regulation of the meristem activity, development of the aboveground and underground parts of plants, and control of embryogenesis. This review examines the role of KNOX and WOX transcription factors in various developmental programs, as well as in the evolutionary complication of the body plan in terrestrial plants.

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“…This might be attributed to the different naming scheme of WOX genes in Arabidopsis and rice [15,23,24]. For example, the TaWOX5 reported by Zhao et al [52] were regarded as TaWOX9 due to their highly similarity to OsWOX9, even though it showed a close similarity to AtWOX5 in all the WOX members in Arabidopsis (Fig. 4).…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification and Expression Profiling Analysis of WOX Family Proteins Encoded Genes in Triticeae Plant Species

Shi

Wang

et al. 2020

Preprint

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Background: Genotype dependence of plant regeneration is an important factor restricting the genetic improvement of Triticeae plant species. The WUSCHEL-related homeobox (WOX) is a group of plant specific transcription factor, which play an important role in plant growth and development, and cell division and differentiation. Recent studies revealed that the application of regeneration-related genes such as WOX and BABY BOOM (BBM) could improve plant regeneration. The application of WOX genes is one of the ways to improve the genetic transformation system of Triticeae and other species, but there are rare studies in this area.Results: From the available genome sequence database, in total 136 WOX transcripts were identified for the Triticeae plants, including 43 in Triticum aestivum, 30 in Triticum turgidum, 25 in Triticum dicoccoides, 17 in Hordeum vulgare, 13 in Aegilops tauschii, and 8 in Triticum urartu. All of the WOX family genes were distributed on the chromosomes of homologous groups 1 to 5 in the six Triticeae species, part of which were confirmed by their specific PCR markers using a set of T. durum-T. aestivum genome D substitution lines. All of the WOX proteins in the six Triticeae species could be grouped into three clades, similar to those in rice (Oryza sativa L.) and Arabidopsis. WOX family members were conserved among these Triticeae plants, all of them contained the conserved HOX DNA-binding homeodomain, and WUS clade members contained the characteristic WUS motif, while only TaWUS and TaWOX9 in all the six Triticeae plant species contained the ERF-associated amphiphilic repression (EAR) motif. The expression profiles of TaWOX genes by quantitative real-time PCR (qPCR) showed obvious difference among WOX family members.Conclusions: Totally 130 WOX genes were identified in the six Triticeae plant species. The WOX family genes were located on the chromosomes in the five homologous groups except groups 6 and 7 in the Triticeae species, and their expression profiles were different in different tissues, indicating that each of them had diverse function. The findings in this study could provide a basis for evolution and functional investigation and practical application of the WOX family genes in Triticeae plant species.

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Characterization and expression analysis of WOX5 genes from wheat and its relatives

Cited by 30 publications

References 36 publications

Identification of the WUSCHEL-Related Homeobox (WOX) Gene Family, and Interaction and Functional Analysis of TaWOX9 and TaWUS in Wheat

Identification of the WUSCHEL-Related Homeobox (WOX) Gene Family, and Interaction and Functional Analysis of TaWOX9 and TaWUS in Wheat

Transcription factors in developmental genetics and the evolution of higher plants

Genome-Wide Identification and Expression Profiling Analysis of WOX Family Proteins Encoded Genes in Triticeae Plant Species

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