Comparative analysis of genome sequences from four strains of the Buchnera aphidicola Mp endosymbion of the green peach aphid, Myzus persicae

Jiang, Zhijie; Jones, Derek; Khuri, Sawsan; Tsinoremas, Nicholas F.; Wyss, Tania; Jander, Georg; Wilson, Alex C. C.

doi:10.1186/1471-2164-14-917

Cited by 40 publications

(51 citation statements)

References 63 publications

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“…Full-length sequences for the predicted salivary proteins were obtained from salivary gland cDNA sequences (Ramsey et al 2007) and the Buchnera Mp genome sequence (Jiang et al 2013). Genes were amplified from cDNA or genomic DNA (GroEL) by PCR and were cloned into Agrobacterium plasmids for plant transformation.…”

Section: Resultsmentioning

confidence: 99%

Suppression of Plant Defenses by a Myzus persicae (Green Peach Aphid) Salivary Effector Protein

Elzinga¹,

Vos²,

Jander³

2014

MPMI

Self Cite

213

203

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The complex interactions between aphids and their host plant are species-specific and involve multiple layers of recognition and defense. Aphid salivary proteins, which are released into the plant during phloem feeding, are a likely mediator of these interactions. In an approach to identify aphid effectors that facilitate feeding from host plants, eleven Myzus persicae (green peach aphid) salivary proteins and the GroEL protein of Buchnera aphidicola, a bacterial endosymbiont of this aphid species, were expressed transiently in Nicotiana tabacum (tobacco). Whereas two salivary proteins increased aphid reproduction, expression of three other aphid proteins and GroEL significantly decreased aphid reproduction on N. tabacum. These effects were recapitulated in stable transgenic Arabidopsis thaliana (Arabidopsis) plants. Further experiments with A. thaliana expressing Mp55, a salivary protein that increased aphid reproduction, showed lower accumulation of 4-methoxyindol-3-ylmethylglucosinolate, callose, and hydrogen peroxide in response to aphid feeding. Mp55-expressing plants also were more attractive for aphids in choice assays. Silencing Mp55 gene expression in M. persicae using RNA interference approaches reduced aphid reproduction on N. tabacum, A. thaliana, and Nicotiana benthamiana. Together, these results demonstrate a role for Mp55, a protein with as yet unknown molecular function, in the interaction of M. persicae with its host plants.

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Section: Resultsmentioning

confidence: 99%

Suppression of Plant Defenses by a Myzus persicae (Green Peach Aphid) Salivary Effector Protein

Elzinga¹,

Vos²,

Jander³

2014

MPMI

Self Cite

213

203

View full text Add to dashboard Cite

show abstract

“…The quantitative PCR reactions (15 ml) were performed in duplicate using IQ TM 2X SYBR Green Supermix (Bio-Rad) on a CFX96 Real-Time PCR Detection System using the cycling conditions described above. The Buchnera gene dnaK was chosen for amplification using quantitative PCR since it is present in single-copy in Buchnera and has been used in previous studies Jiang et al, 2013). The A. glycines ribosomal protein gene RpL7 was amplified as an endogenous control for body mass variations between aphid developmental stages.…”

Section: Relative Quantification Of Buchnera Titrementioning

confidence: 99%

Shifts inBuchnera aphidicoladensity in soybean aphids (Aphis glycines) feeding on virus-infected soybean

Cassone

Redinbaugh

Dorrance

et al. 2015

Insect Molecular Biology

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Vertically transmitted bacterial symbionts are common in arthropods. Aphids undergo an obligate symbiosis with Buchnera aphidicola, which provides essential amino acids to its host and contributes directly to nymph growth and reproduction. We previously found that newly adult Aphis glycines feeding on soybean infected with the beetle-transmitted Bean pod mottle virus (BPMV) had significantly reduced fecundity. We hypothesized that the reduced fecundity was attributable to detrimental impacts of the virus on the aphid microbiome, namely Buchnera. To test this, mRNA sequencing and quantitative real-time PCR were used to assay Buchnera transcript abundance and titre in A. glycines feeding on Soybean mosaic virus-infected, BPMV-infected, and healthy soybean for up to 14 days. Our results indicated that Buchnera density was lower and ultimately suppressed in aphids feeding on virus-infected soybean. While the decreased Buchnera titre may be associated with reduced aphid fecundity, additional mechanisms are probably involved. The present report begins to describe how interactions among insects, plants, and plant pathogens influence endosymbiont population dynamics.

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“…The first obligate symbiont genome sequenced, that of Buchnera of the pea aphid, was most notable for the fact that it had undergone extensive gene loss and contained no novel genes yet did retain genes for biosynthesis of essential amino acids needed by hosts (51). With more genome sequencing, it became apparent that Buchnera genomes in different aphid lineages continue to undergo irreversible gene loss, over long and short time scales (52)(53)(54)(55)(56). Similar ongoing gene loss is evident in every obligate symbiont clade for which multiple genomes have been sequenced (16,57,58).…”

Section: Increased Genetic Incompatabilitymentioning

confidence: 99%

Heritable symbiosis: The advantages and perils of an evolutionary rabbit hole

Bennett

Moran

2015

Proc. Natl. Acad. Sci. U.S.A.

431

515

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Many eukaryotes have obligate associations with microorganisms that are transmitted directly between generations. A model for heritable symbiosis is the association of aphids, a clade of sapfeeding insects, and Buchnera aphidicola, a gammaproteobacterium that colonized an aphid ancestor 150 million years ago and persists in almost all 5,000 aphid species. Symbiont acquisition enables evolutionary and ecological expansion; aphids are one of many insect groups that would not exist without heritable symbiosis. Receiving less attention are potential negative ramifications of symbiotic alliances. In the short run, symbionts impose metabolic costs. Over evolutionary time, hosts evolve dependence beyond the original benefits of the symbiosis. Symbiotic partners enter into an evolutionary spiral that leads to irreversible codependence and associated risks. Host adaptations to symbiosis (e.g., immune-system modification) may impose vulnerabilities. Symbiont genomes also continuously accumulate deleterious mutations, limiting their beneficial contributions and environmental tolerance. Finally, the fitness interests of obligate heritable symbionts are distinct from those of their hosts, leading to selfish tendencies. Thus, genes underlying the host-symbiont interface are predicted to follow a coevolutionary arms race, as observed for genes governing host-pathogen interactions. On the macroevolutionary scale, the rapid evolution of interacting symbiont and host genes is predicted to accelerate host speciation rates by generating genetic incompatibilities. However, degeneration of symbiont genomes may ultimately limit the ecological range of host species, potentially increasing extinction risk. Recent results for the aphidBuchnera symbiosis and related systems illustrate that, whereas heritable symbiosis can expand ecological range and spur diversification, it also presents potential perils.Buchnera | aphid | Muller's ratchet | selection levels | coevolution

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Comparative analysis of genome sequences from four strains of the Buchnera aphidicola Mp endosymbion of the green peach aphid, Myzus persicae

Cited by 40 publications

References 63 publications

Suppression of Plant Defenses by a Myzus persicae (Green Peach Aphid) Salivary Effector Protein

Suppression of Plant Defenses by a Myzus persicae (Green Peach Aphid) Salivary Effector Protein

Shifts inBuchnera aphidicoladensity in soybean aphids (Aphis glycines) feeding on virus-infected soybean

Heritable symbiosis: The advantages and perils of an evolutionary rabbit hole

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