Involvement of SpoVG in hemolysis caused by Bacillus subtilis

Pan, Xingliang; Chen, Xiuzhen; Su, Xiaoyun; Feng, Yuan; Tao, Yong; Dong, Zhiyang

doi:10.1016/j.bbrc.2013.12.069

Cited by 16 publications

(10 citation statements)

References 44 publications

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“…The hemolytic mechanism in Bacillus is not fully understood. However, recent studies have been performed to identify the gene(s) responsible for hemolytic activity [23] [24]. Even though the hemolytic activity of some Bacillus spp., such as Bacillus subtilis, is less than in pathogens [25], these isolates may be considered unsafe for food or personal health care applications until the effect of this virulence factor is either eliminated, modified, or confirmed as causing no harm to the eukaryotic host.…”

Section: Introductionmentioning

confidence: 99%

Safety Properties and Probiotic Potential of Bacillus subtilis KATMIRA1933 and Bacillus amyloliquefaciens B-1895

Algburi¹,

Volski²,

Cugini³

et al. 2016

AiM

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This study reports on the safety and putative probiotic properties of Bacillus amyloliquefaciens B-1895 and Bacillus subtilis KATMIRA1933. According to the bacterial reverse mutation (Ames) test, cell-free supernatants of B. amyloliquefaciens B-1895 and B. subtilis KATMIRA1933 were not mutagenic. The two strains co-aggregated with Escherichia coli and Pseudomonas aeruginosa, and cell-free supernatants inhibited the growth of Streptococcus intermedius and Porphyromonas gingivalis. Endospores of B. amyloliquefaciens B-1895 and B. subtilis KATMIRA1933 were tolerant to 0.3% (w/v) bile salts and survived incubation for 4 h in MRS broth at pH 2.0 to 3.0. The ability of the two strains to produce antimicrobial compounds potentiates their application in health care formulations, personal care products, food and animal feed.

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Section: Introductionmentioning

confidence: 99%

Safety Properties and Probiotic Potential of Bacillus subtilis KATMIRA1933 and Bacillus amyloliquefaciens B-1895

Algburi¹,

Volski²,

Cugini³

et al. 2016

AiM

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“…The ultimate effect of inhibition of PTPs negatively regulating rpoB will depend on the functions of expressed proteins. NPBD suppresses endospore formation in B. subtilis and down-regulates RNA binding protein SpoVG which is associated with cell division and initiation of sporulation [ 12 , 33 ]. Deletion of SpoVG in S. aureus reduces methicillin and vancomycin resistance [ 34 ].…”

Section: Resultsmentioning

confidence: 99%

Antibacterial Profile of a Microbicidal Agent Targeting Tyrosine Phosphatases and Redox Thiols, Novel Drug Targets

2021

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The activity profile of a protein tyrosine phosphatase (PTP) inhibitor and redox thiol oxidant, nitropropenyl benzodioxole (NPBD), was investigated across a broad range of bacterial species. In vitro assays assessed inhibitory and lethal activity patterns, the induction of drug variants on long term exposure, the inhibitory interactions of NPBD with antibiotics, and the effect of plasma proteins and redox thiols on activity. A literature review indicates the complexity of PTP and redox signaling and suggests likely metabolic targets. NPBD was broadly bactericidal to pathogens of the skin, respiratory, urogenital and intestinal tracts. It was effective against antibiotic resistant strains and slowly replicating and dormant cells. NPBD did not induce resistant or drug-tolerant phenotypes and showed low cross reactivity with antibiotics in synergy assays. Binding to plasma proteins indicated lowered in-vitro bioavailability and reduction of bactericidal activity in the presence of thiols confirmed the contribution of thiol oxidation and oxidative stress to lethality. This report presents a broad evaluation of the antibacterial effect of PTP inhibition and redox thiol oxidation, illustrates the functional diversity of bacterial PTPs and redox thiols, and supports their consideration as novel targets for antimicrobial drug development. NPBD is a dual mechanism agent with an activity profile which supports consideration of tyrosine phosphatases and bacterial antioxidant systems as promising targets for drug development.

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“…Lack of SpoVG does not cause severe defects, unless SpoIIB, another protein participating in the engulfment stage of sporulation [117], is also absent, something that shows synergistic action and redundancy of these proteins in spore formation [117,118]. SpoVG is also involved in haemolytic activity caused by B. subtilis [119]. Recently, it was also shown to be essential for the formation of B. anthracis spores, which consist the infectious form of this bacterium [118].…”

Section: The Role Of Atypical Rna-binding Proteins Regulating Adaptive Responses In Gram-positive Bacteriamentioning

confidence: 99%

The role of RNA‐binding proteins in mediating adaptive responses in Gram‐positive bacteria

Christopoulou

Granneman

2021

The FEBS Journal

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Bacteria are constantly subjected to stressful conditions, such as antibiotic exposure, nutrient limitation and oxidative stress. For pathogenic bacteria, adapting to the host environment, escaping defence mechanisms and coping with antibiotic stress are crucial for their survival and the establishment of a successful infection. Stress adaptation relies heavily on the rate at which the organism can remodel its gene expression programme to counteract the stress. RNA-binding proteins mediating co-and post-transcriptional regulation have recently emerged as important players in regulating gene expression during adaptive responses. Most of the research on these layers of gene expression regulation has been done in Gram-negative model organisms where, thanks to a wide variety of global studies, large posttranscriptional regulatory networks have been uncovered. Unfortunately, our understanding of post-transcriptional regulation in Gram-positive bacteria is lagging behind. One possible explanation for this is that many proteins employed by Gram-negative bacteria are not well conserved in Grampositives. And even if they are conserved, they do not always play similar roles as in Gram-negative bacteria. This raises the important question whether Gram-positive bacteria regulate gene expression in a significantly different way. The goal of this review was to discuss this in more detail by reviewing the role of well-known RNA-binding proteins in Gram-positive bacteria and by highlighting their different behaviours with respect to some of their Gram-negative counterparts. Finally, the second part of this review introduces several unusual RNA-binding proteins of Gram-positive species that we believe could also play an important role in adaptive responses.

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Involvement of SpoVG in hemolysis caused by Bacillus subtilis

Cited by 16 publications

References 44 publications

Safety Properties and Probiotic Potential of <i>Bacillus subtilis</i> KATMIRA1933 and <i>Bacillus amyloliquefaciens</i> B-1895

Safety Properties and Probiotic Potential of <i>Bacillus subtilis</i> KATMIRA1933 and <i>Bacillus amyloliquefaciens</i> B-1895

Antibacterial Profile of a Microbicidal Agent Targeting Tyrosine Phosphatases and Redox Thiols, Novel Drug Targets

The role of RNA‐binding proteins in mediating adaptive responses in Gram‐positive bacteria

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Involvement of SpoVG in hemolysis caused by Bacillus subtilis

Cited by 16 publications

References 44 publications

Safety Properties and Probiotic Potential of &lt;i&gt;Bacillus subtilis&lt;/i&gt; KATMIRA1933 and &lt;i&gt;Bacillus amyloliquefaciens&lt;/i&gt; B-1895

Safety Properties and Probiotic Potential of &lt;i&gt;Bacillus subtilis&lt;/i&gt; KATMIRA1933 and &lt;i&gt;Bacillus amyloliquefaciens&lt;/i&gt; B-1895

Antibacterial Profile of a Microbicidal Agent Targeting Tyrosine Phosphatases and Redox Thiols, Novel Drug Targets

The role of RNA‐binding proteins in mediating adaptive responses in Gram‐positive bacteria

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Safety Properties and Probiotic Potential of <i>Bacillus subtilis</i> KATMIRA1933 and <i>Bacillus amyloliquefaciens</i> B-1895

Safety Properties and Probiotic Potential of <i>Bacillus subtilis</i> KATMIRA1933 and <i>Bacillus amyloliquefaciens</i> B-1895