Molecular detection and genetic analysis of porcine bocavirus in Korean domestic swine herds

Choi, M K; Park, Seongjun; Nguyen, Van Giap; Chung, Hee‐Chun; Kim, A-Reum

doi:10.1007/s00705-013-1944-8

Cited by 17 publications

(16 citation statements)

References 16 publications

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“…PBoVs have been detected and characterized by using molecular techniques from arrays of samples which include faeces, serum, lung, lymph nodes and nasopharyngeal samples Lau et al, 2011;Cságola et al, 2012;Blomström et al, 2013;Choi et al, 2014). Although the viral agents have been detected in both symptomatic and asymptomatic pigs, many questions about their pathogenicity are yet to be answered (Gunn et al, 2015).…”

Section: Porcine Bocaviruses (Pbovs)mentioning

confidence: 99%

Increasing diversity of swine parvoviruses and their epidemiology in African pigs

Afolabi

Iweriebor

Okoh

et al. 2019

Infection, Genetics and Evolution

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A B S T R A C TDetection of infectious viral agents has been on the increase globally with the advent and usage of more sensitive and selective novel molecular techniques in the epidemiological study of viral diseases of economic importance to the swine industry. The observation is not different for the pig-infecting member of the subfamily Parvovirinae in the family Parvoviridae as the application of novel molecular methods like metagenomics has brought about the detection of many other novel members of the group. Surprisingly, the list keeps increasing day by day with some of them possessing zoonotic potentials. In the last one decade, not less than ten novel swine-infecting viruses have been added to the subfamily, and ceaseless efforts have been in top gear to determine the occurrence and prevalence of the old and new swine parvoviruses in herds of pig-producing countries worldwide. The story, however, is on the contrary on the African continent as there is presently a dearth of information on surveillance initiatives of the viruses among swine herds of pig-producing countries in the region. Timely detection and characterization of the viral pathogens is highly imperative for the implementation of effective control and prevention of its spread. This review therefore presents a concise overview on the epidemiology of novel porcine parvoviruses globally and also provides up-to-date highlights on the reported cases of the viral agents in the African sub-region.

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Section: Porcine Bocaviruses (Pbovs)mentioning

confidence: 99%

Increasing diversity of swine parvoviruses and their epidemiology in African pigs

Afolabi

Iweriebor

Okoh

et al. 2019

Infection, Genetics and Evolution

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“…So far, there is no internationally recognized genotyping criterion for PBoVs. Based on the homology of NS1 gene of PBoV, some studies classified different PBoV epidemic strains into different clades (PBoV1~PBoV5) (Xiao et al, 2013;Choi et al, 2014). PBoV1 with a genome size of 4,786 bp was detected in pigs suffering from PMWS in Sweden (Blomström et al, 2009), which suggested that PoBoV1 might play some role as co-factor in the development of the disease.…”

Section: Discussionmentioning

confidence: 99%

“…In 2009, Swedish researchers for the first time isolated PBoV from lymph nodes of the piglets with postweaning multisystemic wasting syndrome (PMWS) (Blomström et al, 2009). Since then, PBoV was identified in many countries including the USA, Northern Ireland, Cameroon, Uganda, Korea and some European countries (Cadar et al, 2011;McKillen et al, 2011;McNair et al, 2011;Shan et al, 2011b;Blomström et al, 2013;Ndze et al, 2013;Choi et al, 2014;Huang et al, 2014;Jiang et al, 2014;Gunn et al, 2015). In China, PBoV has also been identified in many regions including Hubei, Shanghai, Jiangsu, Shandong, Guizhou and Hong Kong (Cheng et al, 2010;Zhai et al, 2010;Fu et al, 2011;Zhang et al, 2011;Wang et al, 2013;Luo et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Molecular detection and genetic diversity of porcine bocavirus in piglets in China

Meng¹,

Qiao²,

Gong³

et al. 2018

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Porcine bocavirus (PBoV) is a recently discovered, non-enveloped and single-stranded DNA virus that can infect pigs. In order to understand PBoV infection and its genetic characterization in piglets in Xinjiang China, PBoV was detected by PCR in 156 clinical samples from 1-month-old piglets. PBoV was detected in 9 clinical samples, with a prevalence rate of 5.77% (9/156). Then nonstructural protein NS1 gene was amplified, sequenced and used for phylogenetic analysis. The results showed that the prevalence rate in the sick piglets was 9.33% (7/75), which is significantly higher than that in the healthy piglets (2.47%, 2/81). The nucleotide sequences of NS1 gene share high identities (96.1-99.2%) within the same groups of PBoVs. Phylogenetic analysis based on complete nucleotide sequence of NS1 gene showed that PBoV strains can be classified into three genetic groups, among which group I contains PBoV1 strains, group II contains PBoV2 strains, and group III contains PBoV3, PBoV4 and PBoV5 strains. Porcine/XJ-12, porcine/XJ-27, porcine/XJ-65, and porcine/XJ-145 had close genetic distance with subgroup 1, belonging to group I; strains porcine/XJ-79 and porcine/XJ-134 were clustered with subgroup 2, belonging to group II, while porcine/XJ-8, porcine/XJ-52 and porcine/XJ-96 were clustered with subgroup 3, which belonged to group III. This study demonstrated for the first time that PBoV strains in Xinjiang belong to three subgroups of three different genetic groups, indicating a substantial genetic diversity of the epidemic strains circulating in China, which provided the useful epidemiological data for scientific control and prevention of this disease in farm pigs.

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“…Viruses in the Bocaparvovirus genus belong to the subfamily Parvovirinae of the Parvoviridae family, which is a group of divergent linear ssDNA viruses (Tijssen et al, 2011). Porcine bocavirus has a genome of ~5 kb and has three open reading frames that encode four proteins: NS1, NP1, VP1 and VP2 (Choi et al, 2014;Zeng et al, 2011;Zhang et al, 2015b). Parvoviruses were demonstrated to have nucleotide substitution rates that are as high as those of some RNA viruses (Duffy, Shackelton, and Holmes, 2008;Shackelton et al, 2005) and recent results suggest that the presently circulating PBoVs exhibit considerable genetic diversity within the same sample and between different pigs (Lau et al, 2011;Liu et al, 2014;Shan et al, 2011;Zhang et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Development and validation of a multiplex conventional PCR assay for simultaneous detection and grouping of porcine bocaviruses

Zheng

Liu

Opriessnig

et al. 2016

Journal of Virological Methods

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Porcine bocavirus (PBoV), a newly described porcine parvovirus, has received attention because it can be commonly identified in clinically affected pigs including pigs with post-weaning multisystemic wasting syndrome (PWMS) and pigs with diarrhea. In recent years, novel PBoVs have been identified and were classified into three genogroups, but the ability to detect and classify these novel PBoVs is not comprehensive to date. In this study, a multiplex conventional PCR assay for simultaneous detection and grouping of PBoVs was developed by screening combinations of mixed primer pairs followed by optimization of the PCR conditions. This method exclusively amplifies targeted fragments of 531bp from the VP1 gene of PBoV G1, 291bp from the NP1 gene of PBoV G2, and 384bp from the NP1/VP1 gene of PBoV G3. The assay has a detection limit of 1.0×10(3)copies/μL for PBoV G1 4.5×10(3) for PBoV G2 and 3.8×10(3) for PBoV G3 based on testing mixed purified plasmid constructs containing the specific viral target fragments. The performance of the multiplex PCR assay was comparable to that of the single PCRs which used the same primer pairs. Using the newly established multiplex PCR assay, 227 field samples including faeces, serum and tissue samples from pigs were investigated. All three PBoV genogroups were detected in the clinical samples with a detection rate of 1.3%, 2.6% and 12.3%, respectively for PBoV G1, G2 and G3. Additionally, coinfections with two or more PBoV were detected in 1.7% of the samples investigated. These results indicate the multiplex PCR assay is specific, sensitive and rapid, and can be used for the detection and differentiation of single and multiple infections of the three PBoV genogroups in pigs.

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Molecular detection and genetic analysis of porcine bocavirus in Korean domestic swine herds

Cited by 17 publications

References 16 publications

Increasing diversity of swine parvoviruses and their epidemiology in African pigs

Increasing diversity of swine parvoviruses and their epidemiology in African pigs

Molecular detection and genetic diversity of porcine bocavirus in piglets in China

Development and validation of a multiplex conventional PCR assay for simultaneous detection and grouping of porcine bocaviruses

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