Mediatorless N₂incorporated diamond nanowire electrode for selective detection of NADH at stable low oxidation potential

Abstract

The electrocatalytic properties of a N2 incorporated diamond nanowire (N-DNW) unmodified electrode towards the oxidation of nicotinamide adenine dinucleotide (NADH) was critically evaluated. The electrochemical behavior of the N-DNW unmodified electrode was examined and compared with that of boron-doped diamond, glassy carbon electrode, and graphite electrodes. The N-DNW electrode had high selectivity and high sensitivity for the differential pulse voltammetric detection of NADH in the presence of ascorbic acid at the lower and stable oxidation potential. Moreover, it exhibited strong stability after prolonged usage. The oxidation peak potential at the N-DNW electrode remained unchanged even after exposure to the solution, followed by washing, drying, and storage in laboratory air for 20 days, with minimization of surface contamination. Therefore, the N-DNW unmodified electrode shows promise for the detection of NADH and is attractive for use in a dehydrogenase based biosensor and other analytical applications.

Boron-Enhanced Growth of Micron-Scale Carbon-Based Nanowalls: A Route toward High Rates of Electrochemical Biosensing

“…Thus, A eff for B:CNW is almost 3.6 times higher than the geometric electrode area that provided a conclusive evidence of the 3D porous structure of the investigated electrode (see Figure D). It indicates also that not only surface edges but also side nanowall planes of the sample could have an impact on the electrochemical processes and such material can be employed as an ultrasensitive electrode because of its large active surface area …”

Insight into the Effect of the Core–Shell Microstructure on the Electrochemical Properties of Undoped 3D-Networked Conductive Diamond/Graphite

“…Lin and coauthors have done much research to investigate the effect of N 2 incorporation in the Ar/CH 4 admixture on the growth of the hybrid D/G nanocomposite. − CN species were considered as the essential key for the nanocomposite formation because they would preferentially attach on the tip of the nanowire, promoting the elongation of the diamond grains . Owing to the large apparent surface area and high electrocatalytic activity, great performances in the determination of adenine dinucleotide, dopamine (DA), and urea have been achieved in the electrochemical sensing field. − Besides, Raina et al proposed a nitrogen-incorporated D/G film with a unique “ridge” morphology and demonstrated great sensitivity in the detection of DA and glucose . Very recently, such nitrogen-incorporated D/G nanoplatelet films have been used as biocompatible platforms for electrically assisted proliferation and differentiation because of the cooperation of D/G inherent properties (i.e., excellent biocompatibility, great electrical conductivity, and the unique microstructure with a ridge–trough morphology) .…”

Nitrogen-Doped Diamond Film for Optical Investigation of Hemoglobin Concentration

“…The prominent bands at 1365 cm −1 and 1560 cm −1 are designated as D-band and G-band, respectively, representing the disorder carbon and graphites [ 25 , 26 , 27 , 28 ]. The Raman peak for 1336 cm −1 (D-band) is characteristic for the diamond phase (sp 3 ) [ 29 ]. The peak at 1336 cm −1 is characteristic for the diamond phase (sp 3 ).…”