Cell cycle analysis and interspecies nuclear transfer of cat cells treated with chemical inhibitors

Abstract

This study investigated the effect of chemical inhibitors on the cell-cycle synchronisation in cat fibroblast cells and evaluated the development of interspecies embryos reconstructed from cat donor cells and enucleated bovine oocytes. Cat fibroblast cells were treated with 15 μg/mL roscovitine or 0.05 μg/mL demecolcine prior to cell cycle analysis and nuclear transfer. The percentage of cat fibroblast cells arrested at the G0/G1 phase in the roscovitine group was similar to that in the control group without any treatment. The percentage of cells arrested at the G2/M phase was significantly higher in the demecolcine group than in the control group. The fusion rate of interspecies couplets was significantly greater in the roscovitine group than in the control group. Most embryos stopped the development at the 2-or 4-cell stage, and none developed into blastocysts. Chemical inhibitor-induced donor cell cycle synchronisation did not overcome developmental arrest in interspecies cloned embryos. Key words: Bovine oocyte, cell cycle synchronisation, demecolcine, feline, roscovitineInterspecies somatic cell nuclear transfer (iSCNT) is an alternative way to explore species for which oocytes and recipients are limited; these species include endangered or exotic species (Karja et al., 2006). It has been shown that bovine, sheep and rabbit oocyte cytoplasm supports in vitro development of embryos produced by interspecies nuclear transfer of somatic cells from various unrelated mammalian species, although no pregnancy has lasted to full term after the transfer of iSCNT embryos to surrogate animals (Dominko et al., 1999;White et al., 1999;Chen et al., 2002;Murakami et al., 2005). Moreover, the ability of iSCNT embryos to develop into blastocysts decreases as the taxonomic distance between the donor and recipient species increases (Beyhan et al., 2007).

FOS-like antigen 1 is highly expressed in human psoriasis tissues and promotes the growth of HaCaT cells in vitro

“…A disturbance during cell division can lead to abnormal cell proliferation and dysregulation in molecular signaling is commonly associated with altered cell growth, cell cycle progression and impaired apoptotic responses in diseases (19)(20)(21). Apoptosis is the selective process of physiological cell deletion that regulates the balance between cell proliferation and cell death.…”