Effect of Antibiotics on Redox Transformations of Arsenic and Diversity of Arsenite-Oxidizing Bacteria in Sediment Microbial Communities

Yamamura, Shigeki; Watanabe, Keiji; Suda, Wataru; Tsuboi, Shun; Watanabe, Mirai

doi:10.1021/es403971s

Cited by 34 publications

(19 citation statements)

References 43 publications

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“…40 Unlike arrA, the detoxification reductase genes arsC is present in both aerobic and anaerobic microbes 19,37 and has been identified in environmental samples and rice rhizosphere. 11,30 In this study, the microbes responsible for detoxification As(V) reduction belonged to some typically rhizospheric microbes, such as Rhizobiales and Pseudomonadales (Supporting Information, Figure S7) and could contribute to As(V) reduction and mobility in paddy soils under both aerobic and anaerobic conditions.…”

Section: ■ Discussionmentioning

confidence: 86%

Diversity and Abundance of Arsenic Biotransformation Genes in Paddy Soils from Southern China

Zhang

Zhao

Sun

et al. 2015

Environ. Sci. Technol.

219

114

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Microbe-mediated arsenic (As) biotransformation in paddy soils determines the fate of As in soils and its availability to rice plants, yet little is known about the microbial communities involved in As biotransformation. Here, we revealed wide distribution, high diversity, and abundance of arsenite (As(III)) oxidase genes (aioA), respiratory arsenate (As(V)) reductase genes (arrA), As(V) reductase genes (arsC), and As(III) Sadenosylmethionine methyltransferase genes (arsM) in 13 paddy soils collected across Southern China. Sequences grouped with As biotransformation genes are mainly from rice rhizosphere bacteria, such as some Proteobacteria, Gemmatimonadales, and Firmicutes. A significant correlation of gene abundance between arsC and arsM suggests that the two genes coexist well in the microbial As resistance system. Redundancy analysis (RDA) indicated that soil pH, EC, total C, N, As, and Fe, C/N ratio, SO 4 2− -S, NO 3 − -N, and NH 4 + -N were the key factors driving diverse microbial community compositions. This study for the first time provides an overall picture of microbial communities involved in As biotransformation in paddy soils, and considering the wide distribution of paddy fields in the world, it also provides insights into the critical role of paddy fields in the As biogeochemical cycle. ■ INTRODUCTIONChina is the world's largest rice producer, accounting for about 30% of the total world production (http://beta.irri.org/ statistics), mostly in Southern China.1 As a highly toxic metalloid, arsenic (As) contamination in paddy fields has emerged as a serious health concern worldwide, 2 especially considering that the anaerobic conditions in paddy soils are conducive to As mobilization, 3,4 resulting in a markedly enhanced bioavailability of As to rice plants. 5 It is now recognized that consumption of rice constitutes a large proportion of the dietary intake of As for the populations in China and other Asian countries. 6−8 Microbes are the key drivers for As biotransformation in paddy soils, catalyzing arsenate (As(V)) reduction, arsenite (As(III)) oxidation and methylation.9 Microcosm studies have demonstrated that microbes capable of As reduction, oxidation, and methylation often coexist in paddy soils, and their relative abundance and activity determine the fate of As in the paddy environment and the bioavailability of As to plants. 10−12 There are two known microbial pathways for As(V) reduction, the respiratory pathway mediated by arrA genes 13 and the detoxification pathway mediated by arsC genes.14 As(III) oxidation is catalyzed by As(III) oxidases, which are encoded by aioA and aioB genes for the two subunits of the enzyme 15 and have been identified in several heterotrophic and chemoautotrophic microorganisms. 16 Moreover, As(III) can be methylated by microbes into various organic As species. 17Arsenic biomethylation is catalyzed by As(III) S-adenosylmethionine methyltransferase (ArsM), which is encoded by arsM genes.18

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Section: ■ Discussionmentioning

confidence: 86%

Diversity and Abundance of Arsenic Biotransformation Genes in Paddy Soils from Southern China

Zhang

Zhao

Sun

et al. 2015

Environ. Sci. Technol.

219

114

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“…Bacterial communities can also adapt quickly on hourly or monthly time frames. Only a few studies have considered both the effects on antibiotic exposure on community structure and ecosystem functioning via process rates (Näslund et al 2008;Roose-Amsaleg et al 2013;Underwood et al 2011;Wunder et al 2013;Yamamura et al 2014).…”

Section: Background and Purposesmentioning

confidence: 99%

“…Of these studies, 77 % included processes involved in the nitrogen cycle. The non-nitrogen-related studies involved sulfatereduction (Hansen et al 1992;Ingvorsen et al 2003;Liu et al 2014), pyrene degradation (Näslund et al 2008), iron reduction (Thiele-Bruhn and Beck 2005; Toth et al 2011), arsenic oxidation and reduction (Yamamura et al 2014), methanogenesis (Conkle and White 2012;Fountoulakis et al 2004;Liu et al 2014), and acetate biodegradation kinetics (Wunder et al 2013). We only found a few studies that examined the effect of antibiotics on several biogeochemical processes (Conkle and White 2012;Kotzerke et al 2008;Kotzerke et al 2011;Liu et al 2014;Rosendahl et al 2012;Toth et al 2011).…”

Section: Effects On Biogeochemical Processesmentioning

confidence: 99%

“…The duration of the exposure of colistin used by Bressan et al (2013) on ammonia-oxidizing bacteria was 5 h. Schmidt et al (2012) already stated that Binhibition of cell division or protein biosynthesis may be observed only after test duration of several days.^The importance of incubation time was also shown in the BSubstrate Induced Respiration (SIR)^test of Thiele-Bruhn and Beck (2005); no effect was observed after 4 h, whereas respiration was inhibited by both oxytetracycline and sulfapyridine after 24 h. Short-term experiments that are shorter than the growth rate of the targeted bacteria, therefore, explore the effect of the antibiotic on enzyme activity. Studies regarding short-term effects (Bressan et al 2013;Costanzo et al 2005;Hou et al 2015;Katipoglu-Yazan et al 2013) can be contrasted to long-term effect studies: from days (Ahmad et al 2014;Alighardashi et al 2009;Conkle and White 2012;Fountoulakis et al 2004;Hou et al 2015;Ingvorsen et al 2003;Thiele-Bruhn and Beck 2005;Yamamura et al 2014), weeks (Campos et al 2001;Cui et al 2014;Klaver and Matthews 1994;Kotzerke et al 2008;Kotzerke et al 2011;Lotti et al 2012;Rico et al 2014;Roose-Amsaleg et al 2013;Toth et al 2011;Underwood et al 2011;Wunder et al 2013;Yan et al 2013), months (Fernandez et al 2009;Hansen et al 1992;Näslund et al 2008;Rosendahl et al 2012), to a year (442 days by Schmidt et al 2012). Conducting experiments for extended time periods is difficult because degradable antibiotics, as well as nutrients, can be quickly consumed unless continuously added …”

Section: Duration Of the Experiments: Short-term Versus Long-term Effmentioning

confidence: 99%

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Do antibiotics have environmental side-effects? Impact of synthetic antibiotics on biogeochemical processes

Roose‐Amsaleg

Laverman²

2015

Environ Sci Pollut Res

167

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Antibiotic use in the early 1900 vastly improved human health but at the same time started an arms race of antibiotic resistance. The widespread use of antibiotics has resulted in ubiquitous trace concentrations of many antibiotics in most environments. Little is known about the impact of these antibiotics on microbial processes or "non-target" organisms. This mini-review summarizes our knowledge of the effect of synthetically produced antibiotics on microorganisms involved in biogeochemical cycling. We found only 31 articles that dealt with the effects of antibiotics on such processes in soil, sediment, or freshwater. We compare the processes, antibiotics, concentration range, source, environment, and experimental approach of these studies. Examining the effects of antibiotics on biogeochemical processes should involve environmentally relevant concentrations (instead of therapeutic), chronic exposure (versus acute), and monitoring of the administered antibiotics. Furthermore, the lack of standardized tests hinders generalizations regarding the effects of antibiotics on biogeochemical processes. We investigated the effects of antibiotics on biogeochemical N cycling, specifically nitrification, denitrification, and anammox. We found that environmentally relevant concentrations of fluoroquinolones and sulfonamides could partially inhibit denitrification. So far, the only documented effects of antibiotic inhibitions were at therapeutic doses on anammox activities. The most studied and inhibited was nitrification (25-100 %) mainly at therapeutic doses and rarely environmentally relevant. We recommend that firm conclusions regarding inhibition of antibiotics at environmentally relevant concentrations remain difficult due to the lack of studies testing low concentrations at chronic exposure. There is thus a need to test the effects of these environmental concentrations on biogeochemical processes to further establish the possible effects on ecosystem functioning.

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“…Moreover, the environmental behaviors of antibiotics and arsenite correlate with each other. A recent study indicates that coexisted antibiotics in sediment have a strong effect on the biogeochemical cycle of As (Yamamura et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous removal of tetracycline hydrochloride and As(III) using poorly-crystalline manganese dioxide

et al. 2015

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Effect of Antibiotics on Redox Transformations of Arsenic and Diversity of Arsenite-Oxidizing Bacteria in Sediment Microbial Communities

Cited by 34 publications

References 43 publications

Diversity and Abundance of Arsenic Biotransformation Genes in Paddy Soils from Southern China

Diversity and Abundance of Arsenic Biotransformation Genes in Paddy Soils from Southern China

Do antibiotics have environmental side-effects? Impact of synthetic antibiotics on biogeochemical processes

Simultaneous removal of tetracycline hydrochloride and As(III) using poorly-crystalline manganese dioxide

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