“…To circumvent this technical hurdle, we developed a transplantation procedure that increases the rate of integration of human cortical pyramidal neurons into the neonatal cortex. Specifically we took advantage of the observation that EGTA, added together with the cells during intraventricular injection, can promote integration and migration of mouse and human neurons into the mouse host cortex (Nagashima et al, 2014) ( Figure 1A, S1A). In these conditions, transplanted human ESC-derived cortical pyramidal neurons, examined 2-8 weeks post-transplantation, were found within the cortical gray matter, from deep (5/6) to superficial (2/3) cortical layers, displaying a radial orientation ( Figure 1B, S1B) and expressing markers ( Figure 1C-D, S1C-D) typical of cortical pyramidal neurons (Greig et al, 2013).…”