Expression levels of seven candidate genes in human peripheral blood mononuclear cells and their association with preeclampsia

Martínez‐Fierro, Margarita L.; Carrillo‐Sánchez, Karol; Martinez-Gaytan, Victoria; Cortés-Flores, Raúl; Ochoa-Torres, Mauro; Guerrero, G. G.; Rodríguez‐Sánchez, Iram P.; Cancela-Murrieta, Carlos; Zamudio-Osuna, Michelle; Badillo-Almaraz, Jose I.; Rosa, Claudia Castruita-De la

doi:10.3109/10641955.2013.853777

Cited by 15 publications

(7 citation statements)

References 32 publications

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“…It also was not present in a 2007 nested case‐control study that examined early second‐trimester samples . In addition, the possible predictive role of IL‐15 in PE was challenged by a study that documented the expression profile of peripheral blood monocytes in women with PE and normotensive women: Authors found low expression activity of IL‐15, but they acknowledged possible selection biases …”

Section: Resultsmentioning

confidence: 99%

The role of interleukins in preeclampsia: A comprehensive review

Bellos

Karageorgiou

Kapnias

et al. 2018

American J Rep Immunol

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Preeclampsia is a multi‐system hypertensive disorder of pregnancy, with significant rates of maternal and neonatal morbidity. It represents a major cause of preterm birth, as definitive treatment demands fetal delivery. Although its pathophysiology is complicated, placental hypoxia and endothelial dysfunction constitute established pathogenetic steps of the disease. Inflammation is considered to be a crucial mediator of preeclampsia process, as an imbalance between TH1, TH2, and TH17 immune responses is observed. The present review accumulates current knowledge about the contribution of interleukins in preeclampsia, summarizing the pathways through which each interleukin exerts its function in the disease. Also, the role of genetic polymorphisms is explored and the predictive efficacy of maternal serum interleukin levels is evaluated. Finally, recommendations about the safe interpretation of the outcomes, as well as guidance for future research, are provided.

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Section: Resultsmentioning

confidence: 99%

The role of interleukins in preeclampsia: A comprehensive review

Bellos

Karageorgiou

Kapnias

et al. 2018

American J Rep Immunol

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“…21 Moreover, Martinez-Fierro and colleagues reported significantly lower expression of TGF- in PBMCs as a hallmark of preeclampsia. 22 In contrast, recently in 2017, Wang et al worked on multiple soluble TGF- receptors in addition to sEng. They reported a significant increase in the levels of these soluble receptors in the serum of EOP with resultant disruption of TGF- signaling and vasculogenesis.…”

Section: Discussionmentioning

confidence: 99%

Differential Expression of Placental Growth Factor, Transforming Growth Factor-β and Soluble Endoglin in Peripheral Mononuclear Cells in Preeclampsia

Ali

Khaliq

Zaki

et al. 2019

J Coll Physicians Surg Pak

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“…Furthermore, the level of vascular endothelial growth factor was lower in women who developed PE, compared with those that did not ( 42 ). One previous study produced a series of gene profiles in human peripheral blood mononuclear cells and investigated their association with PE ( 43 ). However, the molecular mechanism of miR-204-5p of targeting these important genes during PIH or PE requires further study.…”

Section: Discussionmentioning

confidence: 99%

An exploratory study into the role of miR-204-5p in pregnancy-induced hypertension

Mei

Huang

et al. 2017

Experimental and Therapeutic Medicine

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The molecular mechanism that leads to pregnancy-induced hypertension (PIH), a pregnancy-specific syndrome, remains poorly understood. It has been suggested that microRNAs (miRNAs) may be potentially useful biomarkers for severe preeclampsia (PE), which is an important condition associated with PIH. The aim of the present study was to identify miR-204 by verifying differentially expressed serum miRNAs in patients with PIH during pregnancy compared with normal controls. Subsequently, the effects of miR-204 on proliferation and apoptosis of human choriocarcinoma (JAR) cells in hypoxic microenvironment were investigated. Previous studies indicated a number of miRNA candidates and the present study validated the expression of eight miRNAs in serum samples using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). A higher expression of miR-204 was identified in patients with PIH. To assess the impact of miR-204 inhibition on hypoxic JAR cells function in vitro, cell proliferation was detected using a Cell Counting Kit-8 assay. The rate of apoptosis and cell cycle progression was then examined by flow cytometry. RT-qPCR confirmed that serum miR-204-5p is more highly expressed in patients with PIH. Further statistical analysis indicated that the survival ratio of JAR cells in hypoxic microenvironments was increased in the miR-204-5p inhibitor group. However, the miR-204-5p inhibitor protected hypoxic JAR cells from apoptosis. The analysis of cell-cycle status demonstrated that the percentage of cells in the G2/G1 phase was larger compared with the control group. The results of the present study suggest that low levels of miR-204-5p may increase cell proliferation and reduce cell apoptosis with cell cycle changes in vitro. Therefore, serum miR-204-5p may be used as a notable biomarker for the diagnosis, prevention and treatment of PIH.

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Expression levels of seven candidate genes in human peripheral blood mononuclear cells and their association with preeclampsia

Cited by 15 publications

References 32 publications

The role of interleukins in preeclampsia: A comprehensive review

The role of interleukins in preeclampsia: A comprehensive review

Differential Expression of Placental Growth Factor, Transforming Growth Factor-β and Soluble Endoglin in Peripheral Mononuclear Cells in Preeclampsia

An exploratory study into the role of miR-204-5p in pregnancy-induced hypertension

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