Identification and Preparation of Sperm for ART

Mehta, Akanksha; Sigman, Mark

doi:10.1016/j.ucl.2013.08.005

Cited by 17 publications

(23 citation statements)

References 76 publications

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“…It is noteworthy that sperm washing removes the seminal plasma and extender but does not separate the sperm from other cells. Thus, the technique is not based on their quality or functional capacity (Mehta and Sigman, 2014) and both motile and non-motile sperm are retained. Sperm preparation for IVF can be done by washing with no deleterious effect on cleavage or blastocyst rates (Avery and Greve, 1995).…”

Section: Discussionmentioning

confidence: 99%

“…Its advantage is the yield of >90% of morphologically normal motile sperm, without other cells or debris. However, the absolute yield of sperm is low because motile spermatozoa in the lower levels of the pellet never reach the interface with the overlying culture medium (reviewed by Mehta and Sigman, 2014). Additionally, functional spermatozoa can come into close cell-to-cell contact with defective sperm or leukocytes by centrifugation, thus causing massive oxidative damages of the sperm plasma membrane by ROS and consequently of sperm functions (Henkel and Schill, 2003).…”

Section: Discussionmentioning

confidence: 99%

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Comparison of different methods of goat sperm selection and capacitation for optimization of assisted reproductive technologies

Olivares

Fonseca

Camargo

et al. 2015

Small Ruminant Research

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This study evaluated different methods of frozen-thawed sperm selection and capacitation in goats for further use in reproductive biotechnologies. In Experiment 1, semen was processed by the following techniques: mini-Percoll, swim-up, or washing by centrifugation. In Experiment 2, mini-Percoll selected-sperm was subjected to capacitation induction by incubation with: 50 g/mL heparin, 10 M, 50 M, or 100 M of sodium nitroprusside (SNP). Motility, vigor, acrosome, and plasma membrane (PM) integrity were evaluated after thawing and after each treatment of sperm selection or capacitation. In Experiment 1, washing by centrifugation presented greater (43%; P < 0.05) spermatozoa recovery rate than the other treatments. The swim-up technique showed the lowest (P < 0.05) progressive motility (41%). Spermatozoa presenting both intact PM (P = 0.0002) and acrosome (P = 0.0004) showed an interaction effect between the buck and swim-up technique. In Experiment 2, the addition of 100 M SNP resulted in greater (P < 0.05) motility and vigor (38%; 4.7), respectively, than did heparin (28%; 4.3). An enhancement (P < 0.05) in vigor was obtained after all treatments in comparison with the evaluation after thawing (3.3). In conclusion, mini-Percoll was better than swim-up for preparing frozen-thawed goat sperm, whereas washing by centrifugation technique presented similar rates to mini-Percoll and could also be used. The use of 100 M SNP resulted in better motility and vigor than heparin treatment.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Comparison of different methods of goat sperm selection and capacitation for optimization of assisted reproductive technologies

Olivares

Fonseca

Camargo

et al. 2015

Small Ruminant Research

View full text Add to dashboard Cite

show abstract

“…These studies point out that the failure to complete the fertilization process, syngamy or early cleavage could be the result of an early paternal effect. To validate and resolve this further in the context of humans, research on understanding the functional role of the male gamete beyond penetration, and unraveling the underlying causes of sperm pathology need to be carried out extensively [43], [44].…”

Section: Discussionmentioning

confidence: 99%

Inhibiting Sperm Pyruvate Dehydrogenase Complex and Its E3 Subunit, Dihydrolipoamide Dehydrogenase Affects Fertilization in Syrian Hamsters

et al. 2014

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Background/AimsThe importance of sperm capacitation for mammalian fertilization has been confirmed in the present study via sperm metabolism. Involvement of the metabolic enzymes pyruvate dehydrogenase complex (PDHc) and its E3 subunit, dihydrolipoamide dehydrogenase (DLD) in hamster in vitro fertilization (IVF) via in vitro sperm capacitation is being proposed through regulation of sperm intracellular lactate, pH and calcium.Methodology and Principal FindingsCapacitated hamster spermatozoa were allowed to fertilize hamster oocytes in vitro which were then assessed for fertilization, microscopically. PDHc/DLD was inhibited by the use of the specific DLD-inhibitor, MICA (5-methoxyindole-2-carboxylic acid). Oocytes fertilized with MICA-treated (MT) [and thus PDHc/DLD-inhibited] spermatozoa showed defective fertilization where 2nd polar body release and pronuclei formation were not observed. Defective fertilization was attributable to capacitation failure owing to high lactate and low intracellular pH and calcium in MT-spermatozoa during capacitation. Moreover, this defect could be overcome by alkalinizing spermatozoa, before fertilization. Increasing intracellular calcium in spermatozoa pre-IVF and in defectively-fertilized oocytes, post-fertilization rescued the arrest seen, suggesting the role of intracellular calcium from either of the gametes in fertilization. Parallel experiments carried out with control spermatozoa capacitated in medium with low extracellular pH or high lactate substantiated the necessity of optimal sperm intracellular lactate levels, intracellular pH and calcium during sperm capacitation, for proper fertilization.ConclusionsThis study confirms the importance of pyruvate/lactate metabolism in capacitating spermatozoa for successful fertilization, besides revealing for the first time the importance of sperm PDHc/ DLD in fertilization, via the modulation of sperm intracellular lactate, pH and calcium during capacitation. In addition, the observations made in the IVF studies in hamsters suggest that capacitation failures could be a plausible cause of unsuccessful fertilization encountered during human assisted reproductive technologies, like IVF and ICSI. Our studies indicate a role of sperm capacitation in the post-penetration events during fertilization.

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“…Consequences of PTX treatment in terms of ART results are contradictory (Mehta & Sigman, 2014). Some studies observing no improvement in outcomes (Abdel Raheem et al, 2013;Dimitriadou et al, 1995;Tournaye, Janssens, Verheyen, Devroey, & Van Steirteghem, 1994), while others demonstrating an improvement in fertilisation rates (Table 7).…”

Section: P T X and Art Clini C Al Outcome Smentioning

confidence: 99%

Does in vitro application of pentoxifylline have beneficial effects in assisted male reproduction?

et al. 2020

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Application of nonspecific phosphodiesterases inhibitors, such as pentoxifylline (PTX), is a strategy utilised to aid sperm selection from immotile sperm samples prior to ICSI. No extensive studies have yet been performed to verify the safety of the clinical outcomes of ICSI after PTX administration. In this article, we summarise the data reported in the literature that assess the implication of in vitro usage of PTX on sperm parameters, as well as clinical outcomes during assisted male reproduction programme.

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Identification and Preparation of Sperm for ART

Cited by 17 publications

References 76 publications

Comparison of different methods of goat sperm selection and capacitation for optimization of assisted reproductive technologies

Comparison of different methods of goat sperm selection and capacitation for optimization of assisted reproductive technologies

Inhibiting Sperm Pyruvate Dehydrogenase Complex and Its E3 Subunit, Dihydrolipoamide Dehydrogenase Affects Fertilization in Syrian Hamsters

Does in vitro application of pentoxifylline have beneficial effects in assisted male reproduction?

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