Cyanines in photodynamic reaction assisted by reversible electroporation—in vitro study on human breast carcinoma cells

Weżgowiec, Joanna; Kotulska, Małgorzata; Saczko, Jolanta; Derylo, Maria B.; Teissié, Justin; Rols, Marie‐Pierre; Orio, Julie; Garbiec, Arnold; Kulbacka, Julita

doi:10.1016/j.pdpdt.2013.04.004

Cited by 15 publications

(9 citation statements)

References 27 publications

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“…In vitro research with use of various cell lines, breast cancer among them, confirm the direct cytotoxic influence of PDT [9][10][11][12]. Both necrosis and apoptosis were observed in cultures affected by a photosensitizer and then irradiated [12].…”

Section: Original Papersmentioning

confidence: 74%

“…However, there are continuous efforts to modify the method in order to use it also to fight deeper lesions, for example quite simple intratissue needles with fibre optics [22]. [9,10,11,14]. However, this does not explain the mechanism of the final effect of PDT, i.e.…”

Section: Discussionmentioning

confidence: 99%

“…Many research centres are currently focusing on assessing the effectiveness of various photosensitizers used during photodynamic reactions on in vitro breast cancer cells, including those from metastatic foci [9][10][11][12][13][14].…”

Section: Original Papersmentioning

confidence: 99%

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Expression of Proapoptotic BAX and TP53 Genes and Antiapoptotic BCL-2 Gene in MCF-7 and T-47D Tumour Cell Cultures of the Mammary Gland After a Photodynamic Therapy with Photolon

Płonka¹,

Latocha²,

Kuśmierz³

et al. 2015

Adv Clin Exp Med

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Background. Breast cancer is the most common malignant tumour in women in the whole world. Despite significant developments in the early diagnosis of breast cancer, there is no effective method which would assure total recovery of the patient. Currently available clinical data and laboratory tests indicate a possibility to introduce photodynamic therapy (PDT) to the supplementary treatment of breast cancer. Objectives. The aim of this study was to assess the influence of PDT with Photolon as a photosensibilizator on the expression of apoptosis associated genes (BCL-2, BAX, TP53) in human breast cancer cell lines, preceded by assessment of survivorship and proliferative activity in the tested cells after PDT. Material and Methods. In the present study human breast cancer cell lines MCF-7 and T-47D were used. Photolon (chlorin e6 complex: PVP 1:1) was used as a photosensitizer. Assessments of survivorship and proliferative activity of cells under the influence of PDT (WST-1 test) were conducted along with the expression of selected genes involved in the process of apoptosis: BCL-2, BAX, TP53 (RT-QPCR). Results. PDT limited both survivorship and proliferative activity of breast cancer cells in the two tested lines. In case of T-47D cell line was found increase of BAX and BCL-2 genes expression after PDT and sustained activity of TP53 gene. Conversely, in MCF-7 cell line a decrease in expression was found for both BAX and TP53 genes, but also an increase of BCL-2 gene expression. Conclusions. A progressing decrease (24, 48 and 72 h after PDT) in the count of culture cells, which suggests the occurrence of apoptosis initiated by a photodynamic reaction with simultaneous increase of BCL-2/BAX index, indicates activation of a different endogenous apoptosis pathway than the one examined, namely pointing to sui-

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Section: Original Papersmentioning

confidence: 74%

Section: Discussionmentioning

confidence: 99%

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Expression of Proapoptotic BAX and TP53 Genes and Antiapoptotic BCL-2 Gene in MCF-7 and T-47D Tumour Cell Cultures of the Mammary Gland After a Photodynamic Therapy with Photolon

Płonka¹,

Latocha²,

Kuśmierz³

et al. 2015

Adv Clin Exp Med

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“…EP was performed using ECM 830 device (BTX Harvard Apparatus, Syngen Biotech, Wroclaw/ Poland), generating electrical pulses with the magnitude of 0-3000 V/cm , 10-600 μs long, in the series of 1-99 pulses separated by the time interval of 100 ms -10 s. The electroporation parameters were: a series of eight electric pulses of 800-1000 V/cm, 100 μs long, with the repetition frequency 1 Hz. Cells in suspension were centrifuged for 5 min at 800 rpm and resuspended in the EP buffer with low electrical conductivity (10 mM phosphate, 1 mM MgCl 2 , 250 mM sucrose, pH 7.4) [19]. Cells were used for experiments after 10 min post electroporation to determine cell membrane conditions called "releasing time".…”

Section: Methodsmentioning

confidence: 99%

The effectiveness of chemotherapy and electrochemotherapy on ovarian cell lines in vitro

Saczko

Piłat

Choromańska³

et al. 2016

neo

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The presented study aimed to evaluate in vitro the effectiveness of improvement standard chemotherapy with bleomycin by electroporation in two various ovarian cancer cell lines. Two human ovarian cell lines OvBH-1 and SKOV-3 were used. The lines were selected because of their resistance to several therapeutic methods. As anticancer drug we use range of concentrations of bleomycin. In EP and ECT experiments different voltage values: from 0 to 1200 V/cm, 8 pulses with duration of 100μs and intervals between pulses 1s long were used. The cells viability after applied treatments was evaluated by MTT assay. The expression of heat shock proteins - HSP27 was examined by immunocytochemical ABC method.The cytotoxicity with different concentrations of bleomycin alone was not significantly decrease in both cell lines. It confirms resistance of these cells to conventional chemotherapy. The highest decrease of cell proliferation was observed after EP with bleomycin after 48h of incubation for 1000 V/cm. The intensity of expression of small heat shock proteins HSP27 slightly increased after ECT in both treated cell lines, in particular in OvBH-1. The presented study indicated that application of electroporation may effectively enhance chemotherapy with bleomycin, particularly in the case of treating ovarian cancer resistant to standard therapy.

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“…The EP supports nonselective transport of nonpermeant molecules into the cell, which can be used for gene transfection or drug delivery [27][28][29]. Electrically supported transport of drugs into cells is known as ECT [4,18,25,27,30]. The most important advantage of ECT is enhanced selectivity of the treatment [27].…”

Section: Introductionmentioning

confidence: 99%

Effects of electrophotodynamic therapy in vitro on human melanoma cells – melanotic (MeWo) and amelanotic (C32)

Choromańska¹,

Kulbacka²,

Rembiałkowska³

et al. 2015

Melanoma Research

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Photodynamic therapy has been considered ineffective for melanomas because of the competition between the absorbance of melanin from the melanoma and the absorbance of photosensitizers at the photosensitizer excitation light wavelength. Melanomas show considerable heterogeneity and resistance to phototherapy. The effectiveness of photodynamic therapy could be intensified by electroporation for enhanced transport of a photosensitizer by transient pores in the membrane. In this study, photodynamic therapy combined with electroporation was tested in vitro on the human melanoma cell lines melanotic melanoma (MeWo) and amelanotic melanoma (C32). Control experiments were conducted on human keratinocytes (HaCaT). Photofrin was used as a photosensitizer. Photosensitizer distribution, cloning efficacy test, comet assay, and assessment of apoptotic proteins were performed. Melanin levels were determined before and after photodynamic therapy. The experiments indicated that electroporation effectively supports the photodynamic method. It was found that photodynamic therapy with electroporation efficiently induces apoptosis in melanotic and amelanotic melanoma cells.

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Cyanines in photodynamic reaction assisted by reversible electroporation—in vitro study on human breast carcinoma cells

Cited by 15 publications

References 27 publications

Expression of Proapoptotic BAX and TP53 Genes and Antiapoptotic BCL-2 Gene in MCF-7 and T-47D Tumour Cell Cultures of the Mammary Gland After a Photodynamic Therapy with Photolon

Expression of Proapoptotic BAX and TP53 Genes and Antiapoptotic BCL-2 Gene in MCF-7 and T-47D Tumour Cell Cultures of the Mammary Gland After a Photodynamic Therapy with Photolon

The effectiveness of chemotherapy and electrochemotherapy on ovarian cell lines in vitro

Effects of electrophotodynamic therapy in vitro on human melanoma cells – melanotic (MeWo) and amelanotic (C32)

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