MYB10plays a major role in the regulation of flavonoid/phenylpropanoid metabolism during ripening ofFragaria×ananassafruits

Medina-Puche, Laura; Cumplido-Laso, Guadalupe; Amil-Ruíz, Francisco; Hoffmann, Thomas; Ring, Ludwig; Rodríguez-Franco, Antonio; Caballero, José L.; Schwab, Wilfried; Muñoz‐Blanco, Juan; Blanco‐Portales, Rosario

doi:10.1093/jxb/ert377

Cited by 265 publications

(301 citation statements)

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“…One-way ANOVA was performed on log-transformed data, and letters indicate significant differences (P , 0.05, LSD post hoc test). Taken together, these results indicate that FaEOBII expression is clearly regulated by both ABA content and auxin content of the fruit receptacle, which provides a putative molecular explanation for both the proposal of Perkins-Veazie (1995) and the findings described by Chai et al (2011), and Medina-Puche et al (2014.…”

Section: The Expression Of Faeobii Is Hormonally Regulated Throughoutsupporting

confidence: 70%

“…However, transient silencing of FaMYB1 gene expression in strawberry receptacles did not change the anthocyanin content in transgenic ripe receptacles (Medina-Puche et al, 2014). Similarly, it was demonstrated that the fruit-specific and ripening-related FaMYB10 gene, which encodes an R2R3 MYB TF, regulates the expression of early and late structural genes of the flavonoid/ phenylpropanoid metabolic pathway (Medina-Puche et al, 2014). Silencing the expression of this TF, in fruit receptacles, reduced the expression of genes involved in anthocyanin and phenylpropanoid volatile production as well as the expression of a putative MYB TF (FaEOBII; Medina-Puche et al, 2014).…”

Section: Discussionmentioning

confidence: 84%

“…Similarly, it was demonstrated that the fruit-specific and ripening-related FaMYB10 gene, which encodes an R2R3 MYB TF, regulates the expression of early and late structural genes of the flavonoid/ phenylpropanoid metabolic pathway (Medina-Puche et al, 2014). Silencing the expression of this TF, in fruit receptacles, reduced the expression of genes involved in anthocyanin and phenylpropanoid volatile production as well as the expression of a putative MYB TF (FaEOBII; Medina-Puche et al, 2014). This suggests a direct connection between FaMYB10, FaEOBII, and the flavonoid/ phenylpropanoid metabolism in ripe fruits.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, by using a heterologous system, it was proposed that the R2R3 MYB TF FaMYB1 plays a repressor role in the expression of phenylpropanoid and anthocyanin structural genes in ripe fruit receptacles (Aharoni et al, 2001). However, transient silencing of FaMYB1 gene expression in strawberry receptacles did not change the anthocyanin content in transgenic ripe receptacles (Medina-Puche et al, 2014). Similarly, it was demonstrated that the fruit-specific and ripening-related FaMYB10 gene, which encodes an R2R3 MYB TF, regulates the expression of early and late structural genes of the flavonoid/ phenylpropanoid metabolic pathway (Medina-Puche et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…According to this proposal, the auxins that are produced by achenes and released to the fruit receptacle would repress the expression of a vast majority of the ripening-related genes (Medina-Escobar et al, 1997;Manning, 1998;Moyano et al, 1998;Blanco-Portales et al, 2002, 2004Raab et al, 2006;Medina-Puche et al, 2014). To determine whether FaEOBII gene expression was under auxin control, we determined the changes in FaEOBII gene expression in unripe fruit receptacles after removing the achenes from the fruit surface.…”

Section: The Expression Of Faeobii Is Hormonally Regulated Throughoutmentioning

confidence: 99%

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An R2R3-MYB Transcription Factor Regulates Eugenol Production in Ripe Strawberry Fruit Receptacles

et al. 2015

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Eugenol is a volatile phenylpropanoid that contributes to flower and ripe fruit scent. In ripe strawberry (Fragaria 3 ananassa) fruit receptacles, eugenol is biosynthesized by eugenol synthase (FaEGS2). However, the transcriptional regulation of this process is still unknown. We have identified and functionally characterized an R2R3 MYB transcription factor (EMISSION OF BENZENOID II [FaEOBII]) that seems to be the orthologous gene of PhEOBII from Petunia hybrida, which contributes to the regulation of eugenol biosynthesis in petals. The expression of FaEOBII was ripening related and fruit receptacle specific, although high expression values were also found in petals. This expression pattern of FaEOBII correlated with eugenol content in both fruit receptacle and petals. The expression of FaEOBII was repressed by auxins and activated by abscisic acid, in parallel to the ripening process. In ripe strawberry receptacles, where the expression of FaEOBII was silenced, the expression of CINNAMYL ALCOHOL DEHYDROGENASE1 and FaEGS2, two structural genes involved in eugenol production, was down-regulated. A subsequent decrease in eugenol content in ripe receptacles was also observed, confirming the involvement of FaEOBII in eugenol metabolism. Additionally, the expression of FaEOBII was under the control of FaMYB10, another R2R3 MYB transcription factor that regulates the early and late biosynthetic genes from the flavonoid/phenylpropanoid pathway. In parallel, the amount of eugenol in FaMYB10-silenced receptacles was also diminished. Taken together, these data indicate that FaEOBII plays a regulating role in the volatile phenylpropanoid pathway gene expression that gives rise to eugenol production in ripe strawberry receptacles.The octoploid cultivated strawberry (Fragaria 3 ananassa) is one of the most economically important, nonclimacteric, soft fruits, in which volatile compounds influence fruit flavor and aroma. Both characteristics contribute to the fruit organoleptic traits and are crucial factors to determine fruit quality.At present, extensive surveys on the components that contribute to strawberry flavor have been performed. In these studies, more than 360 volatiles have been identified (Latrasse, 1991;Nijssen, 1996;Zabetakis and Holden, 1997), but only 15 to 20 of them in wild varieties of strawberry are believed to be essential for sensory quality, together with nonvolatile sugars and organic acids (Schieberle and Hofmann, 1997). In contrast, in cultivated varieties of strawberry, only about six odor-active compounds have been identified as contributors to fruit flavor (Raab et al., 2006;Ulrich et al., 2007). Strawberry aroma is the result of the combined perception of fruity (ethyl butanoate, ethyl hexanoate, and methyl 2-methylbutanoat), green (Z-3-hexenal), sweaty (butanoic acid and 2-methylbutanoic acid), peach-like (g-decalactone), and caramel-like [4-hydroxy-2,5-dimethyl-3(2H)-furanone and 2,5-dimethyl-4-methoxy-3(2H)-furanone] flavor notes (Pyysalo et al., 1979;Larsen and Poll, 1992). Several wild strawb...

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Section: The Expression Of Faeobii Is Hormonally Regulated Throughoutsupporting

confidence: 70%

Section: Discussionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: The Expression Of Faeobii Is Hormonally Regulated Throughoutmentioning

confidence: 99%

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An R2R3-MYB Transcription Factor Regulates Eugenol Production in Ripe Strawberry Fruit Receptacles

et al. 2015

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Ripening and Senescence of Fleshy Fruits

Anwar

Mattoo

Handa³

2018

Postharvest Biology and Nanotechnology

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Abscisic Acid Regulates Fleshy Fruit Ripening and Stress Resistance

Yuan

Leng

2020

Annual Plant Reviews Online

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Abscisic acid (ABA) is an essential endogenous messenger during plant development and in stress responses. Recently, the role of ABA in both climacteric and nonclimacteric fruit ripening regulation was revealed. However, the genetic, molecular and physiological mechanisms of ABA in fruit ripening regulation is still not completely understood. ABA is involved in a wide range of fruit development processes, including fruit ripening onset, ripening process and fruit quality trait. In addition, ABA plays a significant role in responses to abiotic stress and the promotion of water efficiency in fruit. The biological effect of ABA depends on its concentration and signal transduction. This article summarises the latest discoveries of the roles of ABA in fruit development and ripening as well as in responses to abiotic stress.

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MYB10plays a major role in the regulation of flavonoid/phenylpropanoid metabolism during ripening ofFragaria×ananassafruits

Cited by 265 publications

References 59 publications

An R2R3-MYB Transcription Factor Regulates Eugenol Production in Ripe Strawberry Fruit Receptacles

An R2R3-MYB Transcription Factor Regulates Eugenol Production in Ripe Strawberry Fruit Receptacles

Ripening and Senescence of Fleshy Fruits

Abscisic Acid Regulates Fleshy Fruit Ripening and Stress Resistance

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