Antioxidant Potential and Antioxidant Compounds of Extracts from the Medicinal Sulphur Polypore, Laetiporus sulphureus (Higher Basidiomycetes) in Submerged Cultures

Abstract

Fermented products, dried mycelia broth, and mycelia-free broth of Laetiporus sulphureus submerged cultures were extracted with ethanol and hot water under optimal culture conditions and investigated for their antioxidant properties. Ethanolic extracts from dried mycelia broth (EEM) and mycelia-free broth (EEB) and hot water extracts from dried mycelia (HWEM) show extraordinary antioxidant properties for all EC50 values below 10 mg/mL except for EEM, as measured by the scavenging effects on DPPH radicals and chelating effects on ferrous ions. Ascorbic acid is rich in EEB and α-tocopherol, and total flavonoids are abundant in EEM. Total phenols are mainly found in EEB and HWEM. There is a high correlation between total phenol content and EC50 values. The correlation coefficient (R2) of the reducing power is 0.994; the R2 of the scavenging effect on DPPH radicals is 0.992; the R2 of the chelating effects on ferrous ions is 0.949; and the R2 of the scavenging effect on superoxide anions is 0.995. These results suggest that extracts of L. sulphureus fermented products are of considerable application potential in antioxidant-related pharmaceutical industries.

Cytotoxicity of some edible mushrooms extracts over liver hepatocellular carcinoma cells in conjunction with their antioxidant and antibacterial properties

“…Previous studies showed that extracts or chemical constituents obtained from various mushrooms possess DPPH scavenging activities due to their antioxidant compounds. Studies carried out with Laetiporus sulphureus;[ 40 ] Pleurotus ostreatus ;[ 31 41 ] Lentinus edodes , Sparassis crispa and Mycoleptodonoides aitchisonii ;[ 42 43 ] Stereum hirsutum ;[ 44 ] Russula virescens ;[ 45 ] Pleurotus eryngii ;[ 46 ] Macrolepiota procera ;[ 27 47 ] Pleurotus flabellatus ;[ 38 ] Russula laurocerasi ;[ 48 ] Suillus bovinus ;[ 49 ] Phellinus fastuosus , Phellinus grenadensis , Phellinus merrillii and Phellinus badius ;[ 50 ] Lenzites betulina ;[ 51 ] Lactarius piperatus ;[ 52 ] Cordyceps militaris ;[ 53 ] Tricholoma portentosum and Lactarius deliciosus ;[ 54 ] Pholiota nameko [ 55 ] revealed the high DPPH scavenging activities of these fungi correlated with their antioxidant compounds.…”

“…Many researchers have previously examined chelating activities of different extracts of some mushroom species and chemical constituents of different fungi in their studies and achieved positive results. [ 38 40 45 46 57 58 59 60 61 62 ]…”

Evaluation of antioxidant activity of some wild macromycetes

“…За лі те ра тур ни ми да ни ми, лі карські види L. sulphureus і Hypsizygus marmoreus (Peck) H.E. Bigelow де монст ру ва ли вищу АОА за умов ета ноль но го екс т ра гу ван ня, ніж з ви користан ням ін ших роз чин ни ків (Lee et al, 2007;Lung, Huang, 2013).…”

“…М.I. Ланг зі спів ав то ра ми по ві дом ля ють, що AOA L. sulphureus пе ре важ но обу мов ле на саме концен тра ція ми в мі це лії фе ноль них спо лук і фла воно ї дів (Lung et al, 2013). Вод но час фе ноль ні ре чови ни є вто рин ни ми ме та бо лі та ми, їхня кіль кість чи ма лою мі рою ви зна ча єть ся умо ва ми зростан ня.…”

Production of Liquiritigenin with Cell-based Biotransformation and Its Anti-Aging Activity

“…For instance, the fruiting body and exopolysaccharides produced in submerged culture of this fungus have attracted considerable attention due to their biological properties including anti-tumor, anti-oxidant, immunostimulatory and cell proliferation effects [15][16][17][18][19][20]. Thus, some investigators have been focused on optimization of culture conditions in flask culture and bioreactors [21][22][23].…”