Inhibitory Effect of Ethyl Acetate Extract of the Shaggy Inc Cap Medicinal Mushroom, Coprinus comatus (Higher Basidiomycetes) Fruit Bodies on Cell Growth of Human Ovarian Cancer

Abstract

In a previous study, we screened organic extracts of different mushroom mycelia and picked ethyl acetate extract of Coprinus comatus as one of the most active extracts against human ovarian cancer cells. In the current study, we extracted a dry powder of C. comatus fruit bodies using ethyl acetate and examined its effect on the viability of three cell lines originated from human ovarian cancer (ES-2, SKOV-3, and SW-626). This extract was active against all tested cell lines, in a dose-dependent manner (concentrations 50-200 µg/mL, P<0.01). In an attempt to segregate the active fraction, we subjected the extract to chromatography on a silica gel column. The effect of six different fractions and of the crude extract on the viability of ES-2 cells was examined after exposure time of 24 h. Fraction F (last eluted) was significantly more effective than crude extract in the reduction of cell viability (P<0.01). Fraction F was also significantly more active than crude extract in the reduction of viability of SKOV-3 cells. We next identified some of the compounds of fraction F (mainly fatty acids) by gas chromatography-mass spectrometry. In summary, ethyl acetate extract of C. comatus reduced viability of three lines of human ovarian cancer. Fractionation of this extract by a silica gel column enabled the selection of a fraction significantly more active than the original extract.

A Novel Laccase with Potent Antiproliferative and HIV-1 Reverse Transcriptase Inhibitory Activities from Mycelia of MushroomCoprinus comatus

“…Previous studies on C. comatus mainly focus on its polysaccharide extract, antitumor and immunomodulatory activities, fermentation, and so forth. In recent researches, ethyl acetate extract from fruit bodies of C. comatus manifested antiproliferative activity towards human ovarian cancer cell lines [6]. A water-soluble polysaccharide demonstrated inhibitory activity towards Sarcoma 180 tumor cell in mice.…”

Between the Bioactive Extracts of Edible Mushrooms and Pharmacologically Important Nanoparticles: Need for the Investigation of a Synergistic Combination - A Mini Review

“…ethyl acetate extract of C. comatus reduced the viability of the three cell lines of human ovarian cancer [50]. These reports from previous studies suggest that the tendency of producing antitumor drugs from natural sources, especially from mushrooms, is realizable.…”

Epidemiology of Ovarian Cancer: Risk Factors and Prevention

“…Dietary Factors and Chemoprevention: Although there are no reports linking consumption of a high fat diet or grilled meat with OC risk [163], consumption of some food items, such as diets rich in fat content [164,165], preservatives [166], and grilled meat [167] increases the risk for many other cancers in both human and animal models. In contrast, diets rich in cruciferous vegetables [168], green tea [169,170], soy products [171,172], and mushrooms [173,174] have been reported to decrease OC risk. Cruciferous vegetables are rich in glutathione, which eliminates carcinogenic metabolites from the body via Phase 2 drug biotransformation reactions [175].…”