The Winding Road to Pluripotency (Nobel Lecture)

Yamanaka, Shinya

doi:10.1002/anie.201306721

Cited by 25 publications

(17 citation statements)

References 45 publications

(59 reference statements)

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“…Consequently, fibroblasts were the first cells from which induced pluripotent stem cells (iPSCs) were obtained, first from mice (Takahashi and Yamanaka, 2006) and then from human origin (Takahashi et al, 2007). Sir John B. Gurdon and Shinya Yamanaka were awarded the 2012 Nobel Prize in Physiology or Medicine for their research in the area of reprogramming mature cells to become pluripotent (Yamanaka, 2013). Since the discovery of iPSCs, human fibroblasts have been reprogrammed to become endothelial cells in response to defined media and culture conditions, being able to form capillary-like structures in vivo in a Matrigel plug mice model (Margariti et al, 2012).…”

Section: Fibroblastsmentioning

confidence: 99%

Cellular strategies to promote vascularisation in tissue engineering applications

Costa‐Almeida

Granja²,

Soares³

et al. 2014

eCM

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Vascularisation is considered to be one of the greatest challenges in tissue engineering. Different strategies exist but cell-based approaches have emerged as a promising therapy to achieve successful vascularisation. The use of endothelial cells to engineer vascularised tissues has been extensively investigated. This field of research has evolved with the discovery of endothelial progenitor cells, a subpopulation with a high regenerative potential. However, the survival of endothelial cell populations alone seems to be impaired. To overcome this problem, co-culture systems, involving supporting cells, like mural cells, fibroblasts, or more tissue-specific cells have been developed. Endothelial cells benefit from the extracellular matrix components and growth factors produced by the supporting cells, which results in neovessel stabilisation and maturation. The use of endothelial progenitor cells in co-culture systems appears to be a promising strategy to promote vascularisation in approaches of increasing complexity. Herein, the authors provide an overview of the cellular strategies that can be used for increasing vascularisation in tissue engineering and regeneration.

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Section: Fibroblastsmentioning

confidence: 99%

Cellular strategies to promote vascularisation in tissue engineering applications

Costa‐Almeida

Granja²,

Soares³

et al. 2014

eCM

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“…Indeed, mouse embryonic and adult fibroblasts were the first cells from which induced pluripotent stem cells (iPSCs) were generated through the addition of a transcription factor cocktail (Oct3/4, Sox2, c‐Myc and Klf4) under embryonic stem cell culture conditions (Takahashi and Yamanaka, ). After, the same protocol was successfully used to obtain iPSCs from human fibroblasts (Takahashi et al , ) J.B. Gurdon and S. Yamanaka were awarded the 2012 Nobel Prize in Physiology or Medicine for their research in the area of reprogramming mature cells to become pluripotent (Yamanaka, ). This finding led to a new paradigm in biomedical research with potential applications in tissue engineering through patient‐specific cell therapy, as well as in drug screening and in modeling human diseases through patient‐specific iPSCs (Yamanaka and Blau, ).…”

Section: Actors In Manipulating Cell Fate Through Induced Pluripotencmentioning

confidence: 99%

“…After, the same protocol was successfully used to obtain iPSCs from human fibroblasts (Takahashi et al, 2007) J. B. Gurdon and S. Yamanaka were awarded the 2012 Nobel Prize in Physiology or Medicine for their research in the area of reprogramming mature cells to become pluripotent (Yamanaka, 2013). This finding led to a new paradigm in biomedical research with potential applications in tissue engineering through patient-specific cell therapy, as well as in drug screening and in modeling human diseases through patient-specific iPSCs (Yamanaka and Blau, 2010).…”

Section: Actors In Manipulating Cell Fate Through Induced Pluripotencmentioning

confidence: 99%

Fibroblasts as maestros orchestrating tissue regeneration

Costa‐Almeida

Soares

Granja

2017

J Tissue Eng Regen Med

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Fibroblasts constitute a dynamic and versatile population of cells of mesenchymal origin, implicated in both regenerative strategies and pathological conditions. Despite being frequently associated to disease development, particularly through the establishment of fibrotic tissue, fibroblasts hold great potential for tissue engineering and regenerative medicine applications. They are responsible for synthesizing and depositing extracellular matrix components, allowing other cells to settle and migrate along a three-dimensional support and thereby generating an organ-specific architecture. Additionally, they produce bioactive molecules that are involved in several physiological processes, including angiogenesis and tissue repair. Although there seems to be much still to unveil about these fascinating cells they have been attracting increasing interest and are now being intensively explored as a cell source to develop bioengineered tissue constructs or to improve stem cell-based technologies. This review intends to highlight the potential of fibroblasts in orchestrating tissue regeneration, as well as to contribute to uncover uncharted prospective applications of these cells.

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“…4 Yamanaka won the Nobel Prize in Physiology or Medicine in November 2012 for his discovery that overexpression of four transcription factors (Oct4, Sox2, KLF4, and cMyc) could induce somatic cells to form pluripotent stem cells. 5 Pluripotent stem cells are capable of almost limitless selfrenewal and can differentiate into any somatic cell type. This pivotal step of transitioning cells from a differentiated state back to pluripotency has opened the door for new avenues of personalized medicine.…”

Section: Inducing Pluripotencymentioning

confidence: 99%

Therapeutic Transdifferentiation: A Novel Approach for Ischemic Syndromes

Connell¹,

Kodali

Cooke³

2015

Methodist DeBakey Cardiovascular Journal

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The technological development of induced pluripotent stem cells (iPSCs) has overcome many of the limitations of adult and embryonic stem cells. We have found that activation of innate immunity signaling is necessary for this process, as it facilitates epigenetic plasticity in cells by a process called transflammation. More recently, we have discovered that transflammation also facilitates the transdifferentiation of cells directly from one somatic cell type to another. This insight may lead to a promising therapeutic pathway that avoids reverting cells all the way back to pluripotency before achieving a cell type of interest. While there is much therapeutic promise to transflammation and transdifferentiation, there is also evidence that transdifferentiation plays a role in some pathological conditions, including atherosclerosis. Ultimately, better understanding of transflammation will facilitate the development of regenerative therapies.

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The Winding Road to Pluripotency (Nobel Lecture)

Cited by 25 publications

References 45 publications

Cellular strategies to promote vascularisation in tissue engineering applications

Cellular strategies to promote vascularisation in tissue engineering applications

Fibroblasts as maestros orchestrating tissue regeneration

Therapeutic Transdifferentiation: A Novel Approach for Ischemic Syndromes

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