Preparation, Drug Releasing Property and Pharmacodynamics of Soy Isoflavone-Loaded Chitosan Microspheres

Abstract

Soybean isoflavone (SIF) has anti-aging properties and many other biological functions; however, SIF is difficult to reach higher blood concentration due to its rapid metabolism. Therefore, it is of great value to design and produce a sustained-release formulation that is able to maintain a stable level of plasma concentrations. In this paper, soybean isoflavone sustained-release microsphere from chitosan and sodium alginate was prepared successfully. The important factors that determined the quality of the microspheres were the sodium alginate concentration in solution B, the ratio of soybean isoflavone to chitosan and the mixing speed. The relative yield, encapsulation efficiency and drug loading capability of SIF were much higher than the existing commercial formulations. In real gastrointestinal conditions, compared with the non-sustained release group, the release rate of SIF slowed down and the reaction time was prolonged. Animal experiments showed that sustained-release microspheres intensified the anti-aging potentials of SIF. Compared with the Non-sustained release (NSR) group mice, oral SIF/CHI microsphere treated mice were better in the Morris Water Maze Test (MWMT), the MDA level in the both plasma and brain of the sustained release(SR) group mice decreased, and SOD content was remarkably improved.

Ameliorative Effect of Ellagic Acid on Aging in Rats with the Potential Mechanism Relying on the Gut Microbiota and Urolithin A-Producing Ability

“…Consistent with previous studies, d -gal did trigger brain aging in rats, which is manifested by changes in brain connectivity and structures and the behavioral effects as well as the decreased neurotransmitter factor levels. In addition, d -gal-induced oxidative stress can be evaluated by activities of antioxidant enzymes such as GSH-Px, SOD, and CAT and markers of lipid peroxidation (MDA) in rat plasma and brain samples . Moreover, there is a large inflammatory response in addition to oxidative stress damage throughout the aging process.…”

“…In addition, D-gal-induced oxidative stress can be evaluated by activities of antioxidant enzymes such as GSH-Px, SOD, and CAT and markers of lipid peroxidation (MDA) in rat plasma and brain samples. 20 Moreover, there is a large inflammatory response in addition to oxidative stress damage throughout the aging process. Inflammation also occurred, assessed by increased levels of inflammatory factors such as IL-1β, IL-6, and TNF-α in plasma and brain samples, which may be ascribed to D-gal-induced accumulation of ROS.…”

Soy isoflavone-loaded alginate microspheres in thermosensitive gel base: attempts to improve wound-healing efficacy

“…Residues were dissolved in 10 ml ethanol, vortexed (DRAGONLAB, China) for 10 min and filtered through 0.45 μm filter. The filtrate was then analyzed spectrophotometrically (Genesys, Thermo Scientific, China) at 260 nm . Calibration curve for UV assay of SI was constructed using five concentrations ranging from 2.5 to 12.5 μg/ml.…”

“…The filtrate was then analyzed spectrophotometrically (Genesys, Thermo Scientific, China) at 260 nm. [14] Calibration curve for UV assay of SI was constructed using five concentrations ranging from 2.5 to 12.5 lg/ml. Correlation coefficient was nearly equal 0.998.…”