Identification and characterization of the three homeologues of a new sucrose transporter in hexaploid wheat (Triticum aestivum L.)

Deol, Kirandeep K.; Mukherjee, Shalini; Gao, Feng; Brûlé‐Babel, Anita L.; Stasolla, Claudio; Ayele, Belay T.

doi:10.1186/1471-2229-13-181

Cited by 35 publications

(36 citation statements)

References 44 publications

(91 reference statements)

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“…Some of these clones have been shown to be functional SUTs by expressing them in Xenopus oocytes or yeast (Deol et al, 2013; Oner-Sieben et al, 2015; Zanon et al, 2015), but their ability to regulate plant metabolism remains unclear.…”

Section: Introductionmentioning

confidence: 99%

Overexpression of a Grapevine Sucrose Transporter (VvSUC27) in Tobacco Improves Plant Growth Rate in the Presence of Sucrose In vitro

Cai

et al. 2017

Front. Plant Sci.

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The import of sugar from source leaves and it further accumulation in grape berries are considerably high during ripening, and this process is mediated via sucrose transporters. In this study, a grape sucrose transporter (SUT) gene, VvSUC27, located at the plasma membrane, was transferred to tobacco (Nicotiana tabacum). The transformants were more sensitive to sucrose and showed more rapid development, especially roots, when cultured on MS agar medium containing sucrose, considering that the shoot/root dry weight ratio was only half that of the control. Moreover, all transformed plants exhibited light-colored leaves throughout their development, which indicated chlorosis and an associated reduction in photosynthesis. The total sugar content in the roots and stems of transformants was higher than that in control plants. No significant difference was observed in the leaves between the transformants and control plants. The levels of growth-promoting hormones were increased, and those of stress-mediating hormones were reduced in transgenic tobacco plants. The qRT-PCR analysis revealed that the expression of VvSUC27 was 1,000 times higher than that of the autologous tobacco sucrose transporter, which suggested that the markedly increased growth rate of transformants was because of the heterogeneously expressed gene. The transgenic tobacco plants showed resistance to abiotic stresses. Strikingly, the overexpression of VvSUC27 leaded to the up regulation of most reactive oxygen species scavengers and abscisic acid-related genes that might enable transgenic plants to overcome abiotic stress. Taken together, these results revealed an important role of VvSUC27 in plant growth and response to abiotic stresses, especially in the presence of sucrose in vitro.

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Section: Introductionmentioning

confidence: 99%

Overexpression of a Grapevine Sucrose Transporter (VvSUC27) in Tobacco Improves Plant Growth Rate in the Presence of Sucrose In vitro

Cai

et al. 2017

Front. Plant Sci.

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“…Finally, we investigated the molecular basis of the sucrose depletion metabolism in cereal crops. Previous studies in barley, wheat and rice demonstrated that the SUCROSE TRANSPORTER 2 (SUT2) is located in the vacuolar membrane and catalyzes the export of sucrose through a proton coupled co-transport (13, 14, 28, 29). Isolation of the sut2 -mutant in rice provided evidence in planta that SUT2 is necessary for the sucrose export from the vacuole into the cytoplasm as sut2 -mutant plants over-accumulated sucrose in the leaf vacuole at dawn (14).…”

Section: Resultsmentioning

confidence: 99%

“…This confirmed that the sucrose export from the leaf vacuole during the night affects growth and yield. The functional redundancy of the SUT2 transporter in rice, barley and wheat (13, 14, 28, 29) and the predominant consumption of sucrose instead of starch in these species during the night (Figure 5a) together with the growth reduction in cool nights (Figure 5b) suggests that the vacuolar sucrose exporter SUT2 is also a key regulator of the sucrose depletion metabolism and growth in barley and wheat. By contrast, Arabidopsis plants, which accumulate almost exclusively starch in the chloroplast during the day but almost no sucrose in the vacuole (Figure 1b), do not express the homologue of OsSUT2, AtSUT4/SUC4, in the leaf (30).…”

Section: Resultsmentioning

confidence: 99%

Temperature but not the circadian clock determines nocturnal carbohydrate availability for growth in cereals

Jeon

et al. 2018

Preprint

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TRANSPORTER 2 (SUT2), SUCROSE TRANSPORTER 4 (SUT4/SUC4) 27 Abstract (max 250 words): 28The circadian clock is considered a key target for crop improvement because it controls 29 metabolism and growth in Arabidopsis. Here, we show that the clock gene EARLY 30 FLOWERING 3 (ELF3) controls vegetative growth in Arabidopsis but not in the cereal crop 31 barley. Growth in Arabidopsis is determined by the degradation of leaf starch reserves at 32 night, which is controlled by ELF3. The vegetative growth of barley, however, is determined 33 by the depletion of leaf sucrose stores through an exponential kinetics, presumably catalyzed 34 by the vacuolar sucrose exporter SUCROSE TRANSPORTER 2 (SUT2). This process depends 35 on the sucrose content and the nighttime temperature but not on ELF3. The regulation of 36 starch degradation and sucrose depletion in barley ensures efficient growth at favorable 37 temperature as stores become exhausted at dawn. On cool nights, however, only the starch 38 degradation rate is compensated against low nighttime temperatures, whereas the sucrose 39 depletion rate is reduced. This coincides with reduced biomass in barley but not in 40Arabidopsis after growth in consecutive cool nights. The sucrose depletion metabolism 41 determines growth in the cereal crops barley, wheat, and rice but is not generally conserved 42 in monocot species and is not a domestication-related trait. Therefore, the control of growth 43 by endogenous (clock) versus external factors (temperature) is species-specific and depends 44 on the predominant carbohydrate store. Our results give new insights into the physiology of 45 growth in cereals and provide a basis for studying the genetics and evolution of different 46 carbohydrate stores and their contribution to plant productivity and adaptation. 47Significance Statement (120 words max): 48The circadian clock controls growth in the model plant Arabidopsis thaliana by regulating the 49 starch degradation rate so that reserves last until dawn. This prevents nocturnal starvation 50 until photosynthesis resumes. The cereal crops barley, wheat and rice, however, 51 predominantly consume sucrose instead of starch as carbohydrate source. We find that 52 carbohydrate supply from sucrose at night is regulated by enzyme kinetics and night-time 53 temperature, but not the circadian clock. We postulate that the regulation of growth depends 54 on the predominant carbohydrate store, where starch degradation is controlled by 55 endogenous cues (clock) and sucrose depletion by external factors (temperature). These 56 differences in the regulation of carbohydrate availability at night may have important 57 implications for adapting crops yields to climate change. 58 \body 59Introduction 60

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“…For example, StSUT1 of Solanum tuberosum, which is responsible for assimilation loading, also plays an important role in tuber phloem unloading (Kühn et al, 2003). SUT genes cloned from Galega officinalis (Li et al, 2011), Malus pumila (Peng et al, 2011), Triticum aestivum (Deol et al, 2013), and Leymus chinensis (Su et al, 2013) are expressed both in source leaves and sink tissues such as seeds, fruits, and stems. The present experiment revealed that PsSUT1 was critical to the allocation and utilization of sucrose in tree peony.…”

Section: Discussionmentioning

confidence: 99%

Cloning and expression of the sucrose transporter gene PsSUT1 from tree peony leaf

Yh¹,

Guo²,

Yong³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. This study reports the cloning of a sucrose transporter gene, PsSUT1, from the leaf of tree peony (Paeonia suffruticosa Lind. cv 'Huhong'). Expression patterns were examined in different organs and at different developmental stages. The full-length cDNA of PsSUT1 consisted of a 2001-bp sequence containing a 1557-bp open reading frame, encoding 519 amino acids with a conserved domain typical of the glycoside-pentoside-hexuronide superfamily. The amino acid sequence of PsSUT1 in tree peony shared high homology with that of other plants. At different developmental stages, PsSUT1 was expressed in roots, stems, leaves, and petals. Its expression level in stems was 10.9-fold higher than in petals at the flowering stage. Expression of PsSUT1 at the flowering stage was highest during flower development. The significant differences in PsSUT1 expression observed among developmental stages and organs were closely related to changes in sucrose content during flower opening. These results form the basis for further research on the molecular mechanisms of carbohydrate metabolism and transport during flower development in tree peony.

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Identification and characterization of the three homeologues of a new sucrose transporter in hexaploid wheat (Triticum aestivum L.)

Cited by 35 publications

References 44 publications

Overexpression of a Grapevine Sucrose Transporter (VvSUC27) in Tobacco Improves Plant Growth Rate in the Presence of Sucrose In vitro

Overexpression of a Grapevine Sucrose Transporter (VvSUC27) in Tobacco Improves Plant Growth Rate in the Presence of Sucrose In vitro

Temperature but not the circadian clock determines nocturnal carbohydrate availability for growth in cereals

Cloning and expression of the sucrose transporter gene PsSUT1 from tree peony leaf

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