Gastroprotective effects (in rodents) of a flavonoid rich fraction obtained from<i>Syngonanthus macrolepsis</i>

Batista, Leônia Maria; Almeida, Ana Beatriz Albino de; Lima, Gedson Rodrigues de Morais; Falcão, Heloina de Sousa; Magri, Luciana de Pietro; Luiz-Ferreira, Ânderson; Santos, Lourdes Campaner dos; Hiruma-Lima, Clélia Akiko; Vilegas, Wagner; Brito, Alba Regina Monteiro Souza

doi:10.1111/jphp.12175

Cited by 8 publications

(9 citation statements)

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“…Five percent from these numbers suffer from gastric ulcers (Calam and Baron, 2001;Bandyopadhyay et al, 2001). Gastric ulcer is a common disease with multiple etiologies and it is highly prevalent worldwide (Batista et al, 2014). Pathogenesis of gastric ulcers involve stressful lifestyle, alcohol consumption, smoking, infections caused by Helicobacter pylori, usage of nonsteroidal anti-inflammatory drugs (NSAIDs) and drugs that stimulate gastric acid and pepsin secretion (Rang et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Antioxidant and anti-inflammatory activities contribute to the prophylactic effect of semi-purified fractions obtained from the crude methanol extract of Muntingia calabura leaves against gastric ulceration in rats

Balan

Sani

Ahmad

et al. 2015

Journal of Ethnopharmacology

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Section: Introductionmentioning

confidence: 99%

Antioxidant and anti-inflammatory activities contribute to the prophylactic effect of semi-purified fractions obtained from the crude methanol extract of Muntingia calabura leaves against gastric ulceration in rats

Balan

Sani

Ahmad

et al. 2015

Journal of Ethnopharmacology

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“…The gastroprotective activity promoted by flavonoids has been demonstrated in a literature review published by Mota et al [ 30 ]. Studies performed by our group have shown the gastroprotective activity of plant species such as S. arthrotrichus [ 31 ] , S. bisulcatus [ 32 ], and S. macrolepsis [ 33 ] all of which were collected in the state of Minas Gerais, Brazil.…”

Section: Introductionmentioning

confidence: 99%

Ulcer healing and mechanism(s) of action involved in the gastroprotective activity of fractions obtained from Syngonanthus arthrotrichus and Syngonanthus bisulcatus

Batista

Lima

Almeida

et al. 2015

BMC Complement Altern Med

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BackgroundSyngonanthus arthrotrichus and Syngonanthus bisulcatus, currently known for Comanthera aciphylla (Bong.) L.R.Parra & Giul. and Comanthera bisulcata (Koern.) L.R. Parra & Giul, popularly known in Brazil as “sempre-vivas,” are plants from the family Eriocaulaceae. They are found in the states of Minas Gerais and Bahia. The species are known to be rich in flavonoids to which their gastroprotective activity has been attributed. In this research, experimental protocols were performed to elucidate the associated mechanisms of action.MethodsThe activity was evaluated using induced gastric ulcer models (acetic acid and ethanol-induced gastric lesions in NEM or L-NAME pre-treated mice, and by ischemia/reperfusion). Antioxidant enzymes, serum somatostatin, and gastrin were also evaluated.ResultsIn chronic gastric ulcers, a single daily oral dose of Sa-FRF or Sb-FRF (100 mg/kg body wt.) for 14 consecutive days accelerated ulcer healing to an extent similar to that seen with an equal dose of cimetidine. The pre-treatment of mice with NEM (N-ethylmaleimide) or L-NAME (N-nitro-L-arginine) abolished the protective activity of Sa-FRF, Sa-FDF, Sb-FDF and Sb-FRF or Sa-FRF and Sb-FRF, respectively, which indicates that antioxidant compounds and nitric oxide synthase activity are involved in the gastroprotective. Sa-FRF and Sb-FRF (100 mg/kg p.o) protected the gastric mucosa against ulceration that was induced by ischemia/reperfusion (72 and 76 %, respectively). It also decreased lipid peroxidation and restored total thiols in the gastric wall of mice that had been treated with ethanol. When administered to rats submitted to ethanol-induced gastric lesions, Sa-FRF and Sb-FRF (100 mg/kg, p.o.) increased the somatostatin serum levels, while the gastrin serum levels were proportionally decreased.ConclusionsThe results indicate significant healing effects and gastroprotective activity for the Sa-FRF and Sb-FRF, which probably involves the participation of SH groups, nitric oxide (NO), the antioxidant system, somatostatin, and gastrin. All are integral parts of the gastrointestinal mucosa’s cytoprotective mechanisms against aggressive factors.

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“…Jung et al, (2007) reported that the flavonoid-rich fraction from Orostachys japonicus (Crassulaceae) reduced HCl/ ethanol-induced gastric lesions and that the anti-ulcer activity could be attributed to the flavonoid compounds. Batista et al, (2013) demonstrated that the flavonoid-rich fraction from Syngonanthus macrolepsis (Eriocaulaceae) at a dose of 100 mg/kg protected the gastric mucosa in animals, most likely due to the involvement of sulfhydryl compounds and the antioxidant system.In our study, the HPLC-DAD analysis (Figure 3) of the ethyl acetate fraction indicated two major compounds (1 and 2). These substances were isolated and identified by 1 H and 13 C NMR (using CD 3 OD as the solvent).…”

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Gastroprotective activity of the hydroethanolic extract and ethyl acetate fraction from Kalanchoe pinnata (Lam.) Pers.

Sobreira

Hernandes

Vetore-Neto

et al. 2017

Braz. J. Pharm. Sci.

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Peptic ulcers are an important pathology, and the search for safer and more effective treatment methods is of paramount importance. In this study, we assess the gastroprotective effects of the hydroethanolic extract (HE) and ethyl acetate fraction (EAF) from Kalanchoe pinnata leaves against an ethanol/HCl-induced ulcer model in rats. The HE reduced gastric lesions by approximately 47% (400 mg/kg). A significant inhibition of the gastric lesions by 50% was observed after pretreatment with the EAF (200 mg/kg). Quercetrin and quercetin 3-O-α-L-arabinopyranosyl-(1→2)-α-L-rhamnopyranoside were isolated and identified in the flavonoid fraction (EAF) by HPLC and NMR analyses because this fraction showed the highest gastroprotective effect. This fraction demonstrated high antioxidant activities (CE 50 =41.91 µg/mL) by DPPH in comparison with Trolox ® and 11.33 mmol Trolox ® equivalent by ORAC. In conclusion, the HE and FAE from K. pinnata displayed gastroprotective activity in rats, most likely due to the presence of flavonoids.Uniterms: Gastric ulcer/study/rats. Gastric ulcer/treatment. Kalanchoe pinnata/chromatographic profile. Kalanchoe pinnata/gastroprotective activity. Quercetrin. Quercetin 3-O-α-L-arabinopyranosyl-(1→2)-α-L-rhamnopyranoside. INTRODUCTIONFor the past two centuries, peptic ulcers have caused high morbidity and mortality (Malfertheiner, Chan, Mccoll, 2009). This disease is a serious and prevalent pathology (Calam, Baron, 2001). A peptic ulcer is a chronic lesion, usually single, that can be located in the gastric or duodenal mucosae (Kumar, Abbas, Fausto, 2005). In the UK, the incidence of this disease was 12% higher in men than in women (Araujo, Borini, Guimarães, 2014). A review article (January 1980 to February 2009 showed that the incidence rate of uncomplicated peptic ulcers was approximately one case per 1000 people per year in the general population; the incidence rate for complicated peptic ulcers that included bleeding and perforation was approximately 0.7 cases per 1000 people per year (Lin, Rodrígues, Hernándes-Diaz, 2011).The pathophysiology of peptic ulcers suggests that this disease is caused by an imbalance between defensive factors (mucus and bicarbonate secretion, mucosal barrier, blood flow, and prostaglandins) and destructive factors (reactive oxygen species and acid and pepsin secretion), resulting in the acute inflammation of the gastrointestinal mucosae (Laine, Takeuchi, Tarnawski, 2008). Several stimuli serve as offensive factors, including Helicobacter pylori, the use of nonsteroidal anti-inflammatory drugs (NSAIDs), stress, alcohol and cigarette smoking (Malfertheiner, Chan, Mccoll, 2009;Malnick et al., 2014). The current medical treatment for peptic ulcers is generally based on proton pump inhibitors and histamine H 2 -receptor blockers (Jain et al., 2007). However, the long-term use of these therapeutic agents can produce adverse effects, such as hypergastrinemia and hyperplasia in the enterochromaffinlike (ECL) cells, the risk of pneumonia, diarrhea and the disturbance of ...

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Gastroprotective effects (in rodents) of a flavonoid rich fraction obtained fromSyngonanthus macrolepsis

Abstract: The results indicate significant gastroprotective activity for the Sm-FRF, which probably involves the participation of both SH groups and the antioxidant system. Both are integral parts of the gastrointestinal mucosa's cytoprotective mechanisms against aggressive factors.

Cited by 8 publications

References 50 publications

Antioxidant and anti-inflammatory activities contribute to the prophylactic effect of semi-purified fractions obtained from the crude methanol extract of Muntingia calabura leaves against gastric ulceration in rats

Antioxidant and anti-inflammatory activities contribute to the prophylactic effect of semi-purified fractions obtained from the crude methanol extract of Muntingia calabura leaves against gastric ulceration in rats

Ulcer healing and mechanism(s) of action involved in the gastroprotective activity of fractions obtained from Syngonanthus arthrotrichus and Syngonanthus bisulcatus

Gastroprotective activity of the hydroethanolic extract and ethyl acetate fraction from Kalanchoe pinnata (Lam.) Pers.

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