Opposing Dopaminergic and GABAergic Neurons Control the Duration and Persistence of Copulation in Drosophila

Crickmore, Michael A.; Vosshall, Leslie B.

doi:10.1016/j.cell.2013.09.055

Cited by 73 publications

(121 citation statements)

References 46 publications

(47 reference statements)

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“…The rebound is seen in both copulation (58% decrease in satiety) and courtship (84% decrease in satiety) ( Figures S2A and S2B). This finding parallels previous work showing that dopaminergic neurons of the ventral nerve cord (VNC) promote the male's motivation to sustain individual copulation bouts (Crickmore and Vosshall, 2013). In that study, dopaminergic function was localized to the VNC due to the ability of TshGal80 (which inhibits the activity of Gal4 in most VNC neurons) to block Gal4-mediated phenotypes.…”

Section: Dopaminergic Neurons Control Mating Drivesupporting

confidence: 89%

“…To allow copulation but prevent depletion of reproductive fluids from the bulb, we performed a modified satiety assay wherein copulations were artificially terminated 5 min after initiation-before reproductive fluids are transferred (Crickmore and Vosshall, 2013;Gilchrist and Partridge, 2000;Tayler et al, 2012). As expected, the ejaculatory bulb remains full throughout this modified assay ( Figure 2A).…”

Section: Reproductive Satiety In Male Drosophilamentioning

confidence: 85%

“…This reduced potency is evident in males that have recently completed the satiety assay, as their subsequent copulations result in few, if any, progeny ( Figure 1K) (also see Crickmore and Vosshall, 2013;Lefevre and Jonsson, 1962;Linklater et al, 2007). A physical basis of this diminishing fertility is found in the male's ejaculatory bulb, which houses mature sperm and seminal fluid (Demerec, 1950).…”

Section: Reproductive Satiety In Male Drosophilamentioning

confidence: 99%

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Dopaminergic Circuitry Underlying Mating Drive

Zhang

Rogulja

Crickmore

2016

Neuron

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133

208

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We develop a new system for studying how innate drives are tuned to reflect current physiological needs and capacities, and how they affect sensory-motor processing. We demonstrate the existence of male mating drive in Drosophila, which is transiently and cumulatively reduced as reproductive capacity is depleted by copulations. Dopaminergic activity in the anterior of the superior medial protocerebrum (SMPa) is also transiently and cumulatively reduced in response to matings and serves as a functional neuronal correlate of mating drive. The dopamine signal is transmitted through the D1-like DopR2 receptor to P1 neurons, which also integrate sensory information relevant to the perception of females, and which project to courtship motor centers that initiate and maintain courtship behavior. Mating drive therefore converges with sensory information from the female at the point of transition to motor output, controlling the propensity of a sensory percept to trigger goal-directed behavior.

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Section: Dopaminergic Neurons Control Mating Drivesupporting

confidence: 89%

Section: Reproductive Satiety In Male Drosophilamentioning

confidence: 85%

Section: Reproductive Satiety In Male Drosophilamentioning

confidence: 99%

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Dopaminergic Circuitry Underlying Mating Drive

Zhang

Rogulja

Crickmore

2016

Neuron

Self Cite

133

208

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“…GABA immunostaining in Figure S4D was performed as reported (Crickmore and Vosshall, 2013), except PBS replaced PBST.…”

Section: Methodsmentioning

confidence: 99%

Four GABAergic Interneurons Impose Feeding Restraint in Drosophila

Pool

Kvello

Mann

et al. 2014

Neuron

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Summary Feeding is dynamically regulated by the palatability of the food source and the physiological needs of the animal. How consumption is controlled by external sensory cues and internal metabolic state remains under intense investigation. Here, we identify four GABAergic interneurons in the Drosophila brain that establish a central feeding threshold which is required to inhibit consumption. Inactivation of these cells results in indiscriminate and excessive intake of all compounds, independent of taste quality or nutritional state. Conversely, acute activation of these neurons suppresses consumption of water and nutrients. The output from these neurons is required to gate activity in motor neurons that control meal initiation and consumption. Thus, our study reveals a new layer of inhibitory control in feeding circuits that is required to suppress a latent state of unrestricted and non-selective consumption.

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“…Such extreme variability is in agreement with the idea that females assess mates during copulation (although it may also reflect differences in male motivation or spermatophore transfer efficiency). This contrasts with D. melanogaster, where following extensive pre-mating interactions, copulations almost invariably last around 20 min, time affected mostly by male (Economos et al 1979;Taylor et al 2013;Crickmore and Vosshall 2013;and references therein). In T. castaneum, only female copulatory behaviors were found to predict the likelihood of sperm transfer and the number of sperm transferred (Bloch Qazi 2003).…”

Section: Male Copulatory Courtship and Female Quiescence Behaviormentioning

confidence: 86%

Evaluating Cryptic Female Choice in Highly Promiscuous Tribolium Beetles

Fedina

Lewis

2015

Cryptic Female Choice in Arthropods

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Tribolium flour beetles have been an important model organism for the study of postcopulatory sexual selection. These beetles are representative of many insects that live in high-density aggregations and therefore experience frequent mating opportunities. However, unlike many such insects, Tribolium females are extremely polyandrous and seemingly promiscuous as they engage in frequent matings without any apparent pre-mating courtship ritual. Instead, intrasexual competition as well as intersexual mate/paternity choices occurs primarily during and after copulation. Here, we review the evidence for at least four cryptic female choice (CFC) mechanisms in Tribolium that may affect how offspring paternity is apportioned among a female's mates. By comparing live to freshly euthanized females (both readily mated by males), studies reveal that during mating, females reject spermatophore transfer or limit sperm numbers transferred by inferior males. Re-mating with additional males is another CFC mechanism, and mated Tribolium females will more readily accept a spermatophore from males that are more attractive than their previous mate. Finally, Tribolium females may also use the timing of spermatophore ejection after mating to bias offspring paternity. Tribolium life history and mating system traits (including long adult life, continuous egg-laying, and frequent re-mating) suggest that females are unlikely to be harmed by multiple matings. Indeed, there exists little experimental evidence for the cost of polyandry to females, and the Tribolium mating system appears to lack many elements that would indicate sexually antagonistic coevolution. The multiple CFC mechanisms demonstrated in Tribolium may stem from females' inability to effectively assess males before mating. Such mechanisms may provide an effective

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Opposing Dopaminergic and GABAergic Neurons Control the Duration and Persistence of Copulation in Drosophila

Cited by 73 publications

References 46 publications

Dopaminergic Circuitry Underlying Mating Drive

Dopaminergic Circuitry Underlying Mating Drive

Four GABAergic Interneurons Impose Feeding Restraint in Drosophila

Evaluating Cryptic Female Choice in Highly Promiscuous Tribolium Beetles

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