The characteristics of myosin heavy chain-based fiber types in porcine longissimus dorsi muscle

Kim, Gap-Don; Ryu, Youn-Chul; Jo, Cheorun; Lee, Jung‐Gyu; Yang, Han-Sul; Jeong, Jin-Yeon; Joo, Seon-Tea

doi:10.1016/j.meatsci.2013.09.028

Cited by 26 publications

(24 citation statements)

References 28 publications

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“…Muscle fiber size showed significant differences between type I and type IIB, and type IIB was larger than type I regardless of muscle types (p<0.05). Fiber size of hybrid type IIX+IIB was larger than that of another hybrid type, IIA+IIX, in porcine LT muscle, as is consistent with our previous report (Kim et al, 2014). Unlike LT muscle, SM showed an opposite trend in hybrid fiber size: type IIA+IIX (6,631 µm 2 of CSA) was bigger than type IIX+IIB (4,520 µm 2 of CSA).…”

Section: Muscle Fiber Characteristicssupporting

confidence: 91%

“…Thus, 6H1 was not adopted for muscle fiber typing of porcine muscles. Among the four anti-MHC antibodies, BA-F8, BF-35, and BF-F3 showed the same specificity to MHCs as previously observed in porcine muscles (Kim et al, 2014;Lefaucheur et al, 2002;Quiroz-Rothe and Rivero, 2004).…”

Section: Porcine Muscle Fiber Type Identificationsupporting

confidence: 77%

“…Skeletal muscle is generally composed of several types of fiber, which are subdivided by differences in morphological, contractile, and metabolic properties (Kim et al, 2014;Pette and Staron, 2000). Several methods have been used to classify muscle fiber types.…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, they have disadvantages for distinguishing hybrid fiber types. Immunohistochemistry using several antibodies specific to MHC isoforms allows us to identify hybrid fibers (Kim et al, 2014). However, this method requires multiple cross-sections.…”

Section: Introductionmentioning

confidence: 99%

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Muscle Fiber Typing in Bovine and Porcine Skeletal Muscles Using Immunofluorescence with Monoclonal Antibodies Specific to Myosin Heavy Chain Isoforms

Song¹,

Ahn²,

Kim³

2020

Food Sci Anim Resour

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The aim of this study was to optimize staining procedures for muscle fiber typing efficiently and rapidly in bovine and porcine skeletal muscles, such as longissimus thoracis, psoas major, semimembranosus, and semitendinosus muscles. The commercially available monoclonal anti-myosin heavy chain (MHC) antibodies and fluorescent dyeconjugated secondary antibodies were applied to immunofluorescence histology. Two different procedures, such as cocktail and serial staining, were adopted to immunofluorescence analysis. In bovine muscles, three pure types (I, IIA, and IIX) and one hybrid type, IIA+IIX, were identified by the cocktail procedure with a combination of BA-F8, SC-71, BF-35, and 6H1 anti-MHC antibodies. Porcine muscle fibers were typed into four pure types (I, IIA, IIX, and IIB) and two hybrid types (IIA+IIX and IIX+IIB) by a serial procedure with a combination of BA-F8, SC-71, BF-35, and BF-F3. Unlike for bovine muscle, the cocktail procedure was not recommended in porcine muscle fiber typing because of the abnormal reactivity of SC-71 antibody under cocktail procedure. Within the four antibodies, combinations of two or more anti-MHC antibodies allowed us to distinguish pure fiber types or all fiber types including hybrid types. Application of other secondary antibodies conjugated with different fluorescent dyes allowed us to get improved image resolution that clearly distinguished hybrid fibers. Muscle fiber characteristics differed depending on species and muscle types.

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Section: Muscle Fiber Characteristicssupporting

confidence: 91%

Section: Porcine Muscle Fiber Type Identificationsupporting

confidence: 77%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Muscle Fiber Typing in Bovine and Porcine Skeletal Muscles Using Immunofluorescence with Monoclonal Antibodies Specific to Myosin Heavy Chain Isoforms

Song¹,

Ahn²,

Kim³

2020

Food Sci Anim Resour

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“…The three major fiber types (I, IIA and IIB) co‐exist at birth (Choi & Kim ), and their proportions (Wojtysiak & Połtowicz ) in different muscles can influence the metabolic properties of muscles (Choe & Kim ), muscle fiber characteristics (Kim et al . ), meat quality traits (Lim et al . ) and muscle tenderness (Sazili et al .…”

Section: Introductionmentioning

confidence: 99%

Relationships among muscle fiber type composition, fiber diameter and MRF gene expression in different skeletal muscles of naturally grazing Wuzhumuqin sheep during postnatal development

Siqin

Nishiumi

Yamada

et al. 2017

Animal Science Journal

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The aim of this study was to determine the relationships among muscle fiber‐type composition, fiber diameter, and myogenic regulatory factor (MRF) gene expression in different skeletal muscles during development in naturally grazing Wuzhumuqin sheep. Three major muscles (i.e. the Longissimus dorsi (LD), Biceps femoris (BF) and Triceps brachii (TB)) were obtained from 20 Wuzhumuqin sheep and 20 castrated rams at each of the following ages: 1, 3, 6, 9, 12 and 18 months. Muscle fiber‐type composition and fiber diameter were measured using histochemistry and morphological analysis, and MRF gene expression levels were determined using real‐time PCR. In the LD muscle, changes in the proportion of each of different types of fiber (I, IIA and IIB) were relatively small. In the BF muscle, a higher proportion of type I and a 6.19‐fold lower proportion of type IIA fibers were observed (P < 0.05). In addition, the compositions of type I and IIA fibers continuously changed in the TB muscle (P < 0.05). Moreover, muscle diameter gradually increased throughout development (P < 0.05). Almost no significant difference was found in MRF gene expression patterns, which appeared to be relatively stable. These results suggest that changes in fiber‐type composition and increases in fiber size may be mutually interacting processes during muscle development.

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Composition of Muscle Fiber Types in Rat Rotator Cuff Muscles

Rui

Pan²,

2016

The Anatomical Record

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The rat is a suitable model to study human rotator cuff pathology owing to the similarities in morphological anatomy structure. However, few studies have reported the composition muscle fiber types of rotator cuff muscles in the rat. In this study, the myosin heavy chain (MyHC) isoforms were stained by immunofluorescence to show the muscle fiber types composition and distribution in rotator cuff muscles of the rat. It was found that rotator cuff muscles in the rat were of mixed fiber type composition. The majority of rotator cuff fibers labeled positively for MyHCII. Moreover, the rat rotator cuff muscles contained hybrid fibers. So, compared with human rotator cuff muscles composed partly of slow-twitch fibers, the majority of fast-twitch fibers in rat rotator cuff muscles should be considered when the rat model study focus on the pathological process of rotator cuff muscles after injury. Gaining greater insight into muscle fiber types in rotator cuff muscles of the rat may contribute to elucidate the mechanism of pathological change in rotator cuff muscles-related diseases. Anat Rec, 299:1397-1401, 2016. © 2016 Wiley Periodicals, Inc.

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The characteristics of myosin heavy chain-based fiber types in porcine longissimus dorsi muscle

Cited by 26 publications

References 28 publications

Muscle Fiber Typing in Bovine and Porcine Skeletal Muscles Using Immunofluorescence with Monoclonal Antibodies Specific to Myosin Heavy Chain Isoforms

Muscle Fiber Typing in Bovine and Porcine Skeletal Muscles Using Immunofluorescence with Monoclonal Antibodies Specific to Myosin Heavy Chain Isoforms

Relationships among muscle fiber type composition, fiber diameter and MRF gene expression in different skeletal muscles of naturally grazing Wuzhumuqin sheep during postnatal development

Composition of Muscle Fiber Types in Rat Rotator Cuff Muscles

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