An Improve Method for Somatic Embryogenesis of Schisandra chinensis (Turcz.) Baillon

D, Sun; WenXiang, Lang; Li, Hongbo; Guo, Xuewu; Lian, Mei-Lan; Piao, Zhongyun

doi:10.3923/pjbs.2013.127.134

Cited by 3 publications

(3 citation statements)

References 20 publications

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“…The IAA content during this period was lower under the dark treatment than under the light treatment. Under light treatment, the IAA content during the globular embryo period was up to 598.99 ng/g, which is similar to the IAA content in abnormal embryos of S. chinensis(Sun et al 2013). We also observed that more deformed embryos were produced under light culture conditions, possibly due to the high concentration of auxin (597.565 ng•g − 1 ) caused by light in the early stage of somatic embryo development.…”

supporting

confidence: 66%

“…Zhou (2008) found that inducing embryogenic callus of peony in full darkness was more conducive to the induction of embryogenic cells and the formation of somatic embryos. In this study, somatic embryos of S. chinensis treated in the dark for 28 days showed a signi cantly higher rooting rate (95.56%) and germination rate (36.67%) than those treated with light, indicating that the dark environment is suitable for somatic embryo radicle development and indirectly promotes germination (Sun et al 2013). These ndings suggest that dark treatment can improve the rooting and germination rates of somatic embryos in various plant species.…”

Section: Comparison Of S Chinensis Regenerated Plant Rooting Under Da...mentioning

confidence: 55%

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Physiological and molecular mechanisms of radicle development of somatic embryos in Schisandra chinensis cultured in the dark

Sun,

Zhang,

et al. 2024

Plant Cell Tiss Organ Cult

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Somatic embryogenesis (SE) is a method for producing plant embryos in vitro and is considered a highly promising approach for micropropagation. As a valuable Chinese herbal medicine, the application of SE in genetic breeding, such as in Schisandra chinensis, faces several technical challenges, including incomplete development of somatic embryos and di culties in plant regeneration. Here, we established an e cient plant regeneration pathway for somatic embryos in S. chinensis. In this experiment, dark culture conditions were found to signi cantly improve the plant regeneration rooting rate through SE. To understand the genetic mechanism governing embryogenesis, a comparative transcriptome analysis was performed to elucidate differences between light and dark conditions on somatic embryo development in S. chinensis. Dormant buds of S. chinensis were used as explants, and embryonic calli were cultured in light (16 h/D) or dark conditions for 28 days. The cultivation of explants in darkness has been shown to signi cantly enhance the production of somatic embryo radicles. Under dark conditions, radicle primordia were initiated at the globular embryo stage and developed from the heart-shaped to the torpedo-shaped embryo stages. To explore the S. chinensis root mechanism, endogenous hormones were quanti ed, and RNA-seq analysis was performed throughout the process of somatic embryogenesis. The results indicated that from the globular to heart-shaped embryo stages, the levels of IAA and ABA in somatic embryos subjected to the dark treatment were markedly lower (190.965 ng•g − 1 and 525.152 ng•g − 1 ) than those in somatic embryos exposed to light (597.565 ng•g − 1 and 749.188 ng•g − 1 ), while the concentrations of GA 3 and ZR were lower at all stages under light treatment.Transcriptome sequencing and bioinformatics analysis revealed that the pathways and processes in which the differentially expressed genes in somatic embryos under dark conditions were predominantly enriched were plant hormone signaling, circadian rhythm, and phenylpropanoid biosynthesis. qRT-PCR was employed to validate the expression of plant hormone signaling transduction-related genes, including GH3, SAUR, ARF1, ARF18, AUX/IAA, MMK1, AHK4, AHK5, and PIF3, and the results were consistent with the transcriptome sequencing results. This work laid the foundation for applied research and could be useful in future reluctant woody plant improvement programs and can even be extended to other species. Key MessageDark culture signi cantly improved the radicle development of somatic embryos in S. chinensis and the differentially expressed genes were predominantly enriched in plant hormone signals, which corresponded to endogenous hormone levels. 34. Zhou XM (2008) Study on somatic embryogenesis of tree peony. Beijing Forestry University

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confidence: 66%

Section: Comparison Of S Chinensis Regenerated Plant Rooting Under Da...mentioning

confidence: 55%

Physiological and molecular mechanisms of radicle development of somatic embryos in Schisandra chinensis cultured in the dark

Sun,

Zhang,

et al. 2024

Plant Cell Tiss Organ Cult

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“…In vitro cultures of S. chinensis have been the object of research aimed at developing highly effective micropropagation protocols with the use of different initial organs (zygotic embryos, cotyledonary leaves and hypocotyls of germinated zygotic embryos and female flower buds) (Smíšková et al 2005; Kim et al 2005; Chen et al 2010; Yang et al 2011; Sun et al 2013a) (Table 5). …”

Section: Biotechnological Studiesmentioning

confidence: 99%

Current knowledge of Schisandra chinensis (Turcz.) Baill. (Chinese magnolia vine) as a medicinal plant species: a review on the bioactive components, pharmacological properties, analytical and biotechnological studies

Szopa

Ekiert²

2016

Phytochem Rev

262

215

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Schisandra chinensis Turcz. (Baill.) is a plant species whose fruits have been well known in Far Eastern medicine for a long time. However, schisandra seems to be a plant still underestimated in contemporary therapy still in the countries of East Asia. The article presents latest available information on the chemical composition of this plant species. Special attention is given to dibenzo cyclooctadiene lignans. In addition, recent studies of the biological activity of dibenzocyclooctadiene lignans and schisandra fruit extracts are recapitulated. The paper gives a short resume of their beneficial effects in biological systems in vitro, in animals, and in humans, thus underlining their medicinal potential. The cosmetic properties are depicted, too. The analytical methods used for assaying schisandra lignans in the scientific studies and also in industry are also presented. Moreover, special attention is given to the information on the latest biotechnological studies of this plant species. The intention of this review is to contribute to a better understanding of the huge potential of the pharmacological relevance of S. chinensis.

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Physiological and molecular mechanisms of radicle development of somatic embryos in Schisandra chinensis cultured in the dark

SUN,

Zhang,

et al. 2023

Preprint

Self Cite

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Somatic embryogenesis (SE) is a method for producing plant embryos in vitro and is considered a highly promising approach for micropropagation. As a valuable Chinese herbal medicine, the application of SE in genetic breeding, such as in Schisandra chinensis, faces several technical challenges, including incomplete development of somatic embryos and difficulties in plant regeneration. Here, we established an efficient plant regeneration pathway for somatic embryos in S. chinensis. In this experiment, dark culture conditions were found to significantly improve the plant regeneration rooting rate through SE. To understand the genetic mechanism governing embryogenesis, a comparative transcriptome analysis was performed to elucidate differences between light and dark conditions on somatic embryo development in S. chinensis. Dormant buds of S. chinensis were used as explants, and embryonic calli were cultured in light (16 h/D) or dark conditions for 28 days. The cultivation of explants in darkness has been shown to significantly enhance the production of somatic embryo radicles. Under dark conditions, radicle primordia were initiated at the globular embryo stage and developed from the heart-shaped to the torpedo-shaped embryo stages. To explore the S. chinensis root mechanism, endogenous hormones were quantified, and RNA-seq analysis was performed throughout the process of somatic embryogenesis. The results indicated that from the globular to heart-shaped embryo stages, the levels of IAA and ABA in somatic embryos subjected to the dark treatment were markedly lower (190.965 ng•g− 1 and 525.152 ng•g− 1) than those in somatic embryos exposed to light (597.565 ng•g− 1 and 749.188 ng•g− 1), while the concentrations of GA3 and ZR were lower at all stages under light treatment. Transcriptome sequencing and bioinformatics analysis revealed that the pathways and processes in which the differentially expressed genes in somatic embryos under dark conditions were predominantly enriched were plant hormone signaling, circadian rhythm, and phenylpropanoid biosynthesis. qRT‒PCR was employed to validate the expression of plant hormone signaling transduction-related genes, including GH3, SAUR, ARF1, ARF18, AUX/IAA, MMK1, AHK4, AHK5, and PIF3, and the results were consistent with the transcriptome sequencing results. This work laid the foundation for applied research and could be useful in future reluctant woody plant improvement programs and can even be extended to other species.

show abstract

An Improve Method for Somatic Embryogenesis of Schisandra chinensis (Turcz.) Baillon

Cited by 3 publications

References 20 publications

Physiological and molecular mechanisms of radicle development of somatic embryos in Schisandra chinensis cultured in the dark

Physiological and molecular mechanisms of radicle development of somatic embryos in Schisandra chinensis cultured in the dark

Current knowledge of Schisandra chinensis (Turcz.) Baill. (Chinese magnolia vine) as a medicinal plant species: a review on the bioactive components, pharmacological properties, analytical and biotechnological studies

Physiological and molecular mechanisms of radicle development of somatic embryos in Schisandra chinensis cultured in the dark

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