Isobolographic Analysis in Mice of the Interaction of Gabapentin and Nortriptyline in Relieving Orofacial Pain

Miranda, Hugo F.; Noriega, Viviana; Zanetta, Pilar; Prieto, Juan Carlos

doi:10.11607/jop.1167

Cited by 4 publications

(1 citation statement)

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“…An isobolographic analysis [ 20 ] was performed to investigate the pharmacological interactions between DDS and CBD. According to Miranda et al [ 21 ], combinations of fixed proportions were made with the EC 50 -DDS and EC 50 -CBD values, and the culture medium was supplemented with the following mixture of DDS–CBD at proportions: 1:1, 1:4, and 4:1, respectively. Mixture concentrations were determined using the following formulas for each drug: 1:1, EC 50 = 0.5 (EC 50 DDS) + (1 − 0.5) (EC 50 CBD); 1:4, EC 50 = 0.25 (CE 50 DDS) + (1 − 0.25) (EC 50 CBD); 4:1, CE 50 = 0.75 (EC 50 DDS) + (1 − 0.75) (EC 50 CBD).…”

Section: Methodsmentioning

confidence: 99%

Isobolographic Analysis of the Cytoprotective Effect of Dapsone and Cannabidiol Alone or Combination upon Oxygen–Glucose Deprivation/Reoxygenation Model in SH-SY5Y Cells

Islas-Cortez,

Ríos,

Manzanares

et al. 2024

Antioxidants

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Oxidative stress and apoptosis cell death are critical secondary damage mechanisms that lead to losing neighboring healthy tissue after cerebral ischemia. This study aims to characterize the type of interaction between dapsone (DDS) and cannabidiol (CBD) and its cytoprotective effect in an in vitro model of oxygen and glucose deprivation for 6 h followed by 24 h of reoxygenation (OGD/R), using the SH-SY5Y cell line. For the combined concentrations, an isobolographic study was designed to determine the optimal concentration–response combinations. Cell viability was evaluated by measuring the lactate dehydrogenase (LDH) release and 3-[4, 5-dimethyl-2-thiazolyl]-2, 5-diphenyl-2H-tetrazolium bromide (MTT) assays. Also, the reactive oxygen species (ROS) and reduced glutathione (GSH) levels were analyzed as oxidative stress markers. Finally, caspase-3 activity was evaluated as a marker cell death by apoptosis. The results showed a decrease in cell viability, an increase in oxidant stress, and the activity of caspase-3 by the effect of OGD/R. Meanwhile, both DDS and CBD demonstrated antioxidant, antiapoptotic, and cytoprotective effects in a concentration–response manner. The isobolographic study indicated that the concentration of 2.5 µM of DDS plus 0.05 µM of CBD presented a synergistic effect so that in treatment, cell death due to OGD/R decreased. The findings indicate that DDS–CBD combined treatment may be a helpful therapy in cerebral ischemia with reperfusion.

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Section: Methodsmentioning

confidence: 99%

Isobolographic Analysis of the Cytoprotective Effect of Dapsone and Cannabidiol Alone or Combination upon Oxygen–Glucose Deprivation/Reoxygenation Model in SH-SY5Y Cells

Islas-Cortez,

Ríos,

Manzanares

et al. 2024

Antioxidants

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Regulation of Calcium-Independent Phospholipase A2 Expression by Adrenoceptors and Sterol Regulatory Element Binding Protein—Potential Crosstalk Between Sterol and Glycerophospholipid Mediators

Chew

Ong

2014

Mol Neurobiol

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Calcium-independent phospholipase A2 (iPLA2) is an 85-kDa enzyme that releases docosahexaenoic acid (DHA) from glycerophospholipids. DHA can be metabolized to resolvins and neuroprotectins that have anti-inflammatory properties and effects on neural plasticity. Recent studies show an important role of prefrontal cortical iPLA2 in hippocampo-prefrontal cortical LTP and antidepressant-like effect of the norepinephrine reuptake inhibitor (NRI) antidepressant, maprotiline. In this study, we elucidated the cellular mechanisms through which stimulation of adrenergic receptors could lead to increased iPLA2 expression. Treatment of SH-SY5Y neuroblastoma cells with maprotiline, another tricyclic antidepressant with noradrenaline reuptake inhibiting properties, nortriptyline, and the adrenergic receptor agonist, phenylephrine, resulted in increased iPLA2β mRNA expression. This increase was blocked by inhibitors to alpha-1 adrenergic receptor, mitogen-activated protein (MAP) kinase or extracellular signal-regulated kinase (ERK) 1/2, and sterol regulatory element-binding protein (SREBP). Maprotiline and phenylephrine induced binding of SREBP-2 to sterol regulatory element (SRE) region on the iPLA2 promoter, as determined by electrophoretic mobility shift assay (EMSA). Together, results indicate that stimulation of adrenoreceptors causes increased iPLA2 expression via MAP kinase/ERK 1/2 and SREBP, and suggest a possible mechanism for effect of CNS noradrenaline on neural plasticity and crosstalk between sterol and glycerophospholipid mediators, that may play a role in physiological or pathophysiological processes in the brain and other organs.

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Role of prefrontal cortical calcium-independent phospholipase A 2 in antinociceptive effect of the norepinephrine reuptake inhibitor antidepresssant maprotiline

et al. 2017

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Isobolographic Analysis in Mice of the Interaction of Gabapentin and Nortriptyline in Relieving Orofacial Pain

Cited by 4 publications

References 0 publications

Isobolographic Analysis of the Cytoprotective Effect of Dapsone and Cannabidiol Alone or Combination upon Oxygen–Glucose Deprivation/Reoxygenation Model in SH-SY5Y Cells

Isobolographic Analysis of the Cytoprotective Effect of Dapsone and Cannabidiol Alone or Combination upon Oxygen–Glucose Deprivation/Reoxygenation Model in SH-SY5Y Cells

Regulation of Calcium-Independent Phospholipase A2 Expression by Adrenoceptors and Sterol Regulatory Element Binding Protein—Potential Crosstalk Between Sterol and Glycerophospholipid Mediators

Role of prefrontal cortical calcium-independent phospholipase A 2 in antinociceptive effect of the norepinephrine reuptake inhibitor antidepresssant maprotiline

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