Characterization and genetic mapping of a Photoperiod-sensitive dwarf 1 locus in rice (Oryza sativa L.)

Abstract

Plant height is an important agronomic trait for crop architecture and yield. Most known factors determining plant height function in gibberellin or brassinosteroid biosynthesis or signal transduction. Here, we report a japonica rice (Oryza sativa ssp. japonica) dominant dwarf mutant, Photoperiod-sensitive dwarf 1 (Psd1). The Psd1 mutant showed impaired cell division and elongation, and a severe dwarf phenotype under long-day conditions, but nearly normal growth in short-day. The plant height of Psd1 mutant could not be rescued by gibberellin or brassinosteroid treatment. Genetic analysis with R1 and F2 populations determined that Psd1 phenotype was controlled by a single dominant locus. Linkage analysis with 101 tall F2 plants grown in a long-day season, which were derived from a cross between Psd1 and an indica cultivar, located Psd1 locus on chromosome 1. Further fine-mapping with 1017 tall F2 plants determined this locus on an 11.5-kb region. Sequencing analysis of this region detected a mutation site in a gene encoding a putative lipid transfer protein; the mutation produces a truncated C-terminus of the protein. This study establishes the genetic foundation for understanding the molecular mechanisms regulating plant cell division and elongation mediated by interaction between genetic and environmental factors.

Non-specific lipid transfer proteins in maize

“…Cluster I has the least expression variability from 1.01% to 26.80%, indicating a most stable expression pattern relative to other ZmLTP genes. ZmLTP1.1 , the most changeable genes in cluster I, whose homolog in rice, Photoperiod-sensitive dwarf 1 ( Psd1 , Os01g60740 ), was previous demonstrated to encode a lipid transfer protein that may participate in regulation of plant cell division and elongation [39]. One gene ( ZmLTP1.2 ), which can bind to calmodulin in a Ca 2+ -independent manner [26], displayed high expression levels at nearly all of the maize organs and/or stages of development analyzed except for root, immature cob and endosperm.…”

QTLs for Shoot Length and Chlorophyll Content of Rice Seedlings Grown under Low-Temperature Conditions, using a Cross between Indica and Japonica Cultivars

“…The most effective QTL in increasing the shoot length via the Arroz da Terra allele, qSL1, was detected in the Chr .1 2 3 4 5 6 7 8 9 10 11 12 RM5423 RM8105 RM3233 RM2770 RM4355 RM4108 RM1248 RM1200 RM5579 RM6517 RM6467 RM3408 RM5752 RM1353 RM7479 RM8010 RM6369 RM6356 RM8018 RM1148 RM3892 RM7492 RM3882 RM7217 RM286 RM1812 et al, 2000), D10 (Arite et al, 2007), THIS1 (Liu et al, 2013), Psd1 (Li et al, 2014), CIGR (Kovi et al, 2011), SD1 (Sasaki et al, 2002;Ashikari et al, 2005. Yano et al, 2012 and SPS (Ishimaru et al, 2004).…”

Heterologous over-expression of ACC SYNTHASE8 (ACS8) in Populus tremula x P. alba clone 717-1B4 results in elevated levels of ethylene and induces stem dwarfism and reduced leaf size through separate genetic pathways

“…Due to ornamental value and to wide-ranging applications within agriculture, the genetic traits that control cell size and dwarfism in plants have been widely studied (Valdovinos et al, 1967; Ephritikhine et al, 1999; Busov et al, 2003; Qi et al, 2011; Luo et al, 2013; Li et al, 2014; Yang et al, 2014; Zhang et al, 2014). Through breeding practices and applications of growth regulators, dozens of different dwarf plant varieties have been produced over the past few decades (Parker, 2012; Jiang et al, 2013; Wang et al, 2014a,b).…”

“…Leaf size, meanwhile, is linked to productivity, predation (Faeth, 1991) and the water status of the plant (Scoffoni et al, 2011). While both height and leaf size are complex traits, they appear to be genetically regulated by a similar panel of plant hormones (Valdovinos et al, 1967; Ephritikhine et al, 1999; Qi et al, 2011; Luo et al, 2013) and cytochrome P450s (Zhang et al, 2014), as well as abiotic factors such as temperature (Yang et al, 2014) and photoperiod (Li et al, 2014). Reductions in organ size are a result of two different physiological phenomena: smaller cells and impeded cellular division (Beemster et al, 2003).…”