Simultaneous quantification of antimicrobial agents for multidrug-resistant bacterial infections in human plasma by ultra-high-pressure liquid chromatography–tandem mass spectrometry

Tsai, I‐Lin; Sun, Hsin‐Yun; Chen, Guan-Yuan; Lin, Shu-Wen; Kuo, Ching‐Hua

doi:10.1016/j.talanta.2013.07.043

Cited by 78 publications

(59 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The current UHPLC-MS/MS method is superior to the PETINIA technique as it requires only 2 µL of sample compared to the large volumes (50 µL to fill the sample cuvette) required by the immunoassay. Other LC-MS/MS assays determined VAN in human [8] or rat plasma [7], and human serum [6].…”

Section: Resultsmentioning

confidence: 99%

“…These methods were successfully used to measure VAN in a variety of matrices, but they had their limitations. They either were not sufficiently precise [3], suffered from interference [4], had insufficient sensitivity [5] [6] [8], or required large sample volumes [6] [8] [7] [9]. Large sample volumes are sometimes difficult to obtain, especially in studies where VAN is determined in the samples of small laboratory animals, such as rabbits, where the availability of the biological matrix is limited.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

An Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry Method for the Quantification of Vancomycin Requiring Only 2 µL of Rabbit Serum

Schmitt¹,

Szeitz²,

Klassen³

et al. 2017

AJAC

View full text Add to dashboard Cite

A highly sensitive ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was developed for the quantification of vancomycin (VAN) in low volumes of rabbit serum. For each analysis, 2 µL of rabbit serum was precipitated with methanol that contained the internal standard teicoplanin (TEI). The supernatant was transferred into a 384 well-plate, diluted with water, covered with a pierceable silicone mat and 5 µL was analyzed in positive ionization mode. The UHPLC-MS/MS consisted of an Agilent 1290 Infinity UHPLC system connected to an AB Sciex QTrap ® 5500 hybrid linear ion-trap triple quadrupole mass spectrometer equipped with a Turbo Spray source. Chromatographic separation was achieved using a Waters Acquity UPLC BEH C 18 (1.7 µm, 2.1 mm × 100 mm) column, a VanGuard (1.7 µm, 2.1 × 5 mm) guard column and a mobile phase of water and methanol both containing 5 mM ammonium acetate with 0.1% formic acid. VAN was quantified with multiple reaction monitoring using the transitions of m/z 725.5/144.2, and TEI was monitored at m/z 940.6/316.4. The accuracy, precision, linearity, range and lower limit of quantification (LLOQ) were determined. The accuracy was ≤9.93% and the precision was ≤10.6%. The range was established as 0.1 to 40 µg•mL −1 . The LLOQ was 0.1 µg•mL −1 VAN requiring 2 µL of sample with an accuracy of −20.2% and precision of 8.39%. The method was applied successfully to determine the VAN concentrations in rabbit serum after the i.v. administration of VAN.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

An Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry Method for the Quantification of Vancomycin Requiring Only 2 µL of Rabbit Serum

Schmitt¹,

Szeitz²,

Klassen³

et al. 2017

AJAC

View full text Add to dashboard Cite

show abstract

“…Whereas no further processing of the extracted samples is required if analytes' separation is performed in hydrophilic interaction liquid chromatography (HILIC) mode [71]; another important step to perform prior to analysis is the dilution of the supernatant obtained after precipitation, whenever separation is performed in the reversed phase mode [68,74,78]. The dilution step is indispensable to ensure good peaks shape and to maintain the retention time of β-lactams [68,74,78]. Both water and water containing 0.1% formic acid are used.…”

Section: Sample Treatmentmentioning

confidence: 99%

“…As the effect of methanol on individual β-lactams (possibly differential effects) is lacking, further studies are warranted. In all cases, eluents are supplemented with small amounts of acids and salts, necessary to obtain reproducible peak areas as well as to improve ionization once the sample reaches the MS [78]. However, it is worth mentioning that although small amounts of acids have been used for the analysis of β-lactams, the presence of acids favors the formation of sodium adducts of other antibiotic such as sulfamethoxazole and in this case the use of acids is not highly recommended [67].…”

Section: Lc Separationmentioning

confidence: 99%

“…Furthermore, both TDM and an increase in the Cmin, trough concentration; Cmax, maximum concentration. [78,79] collaboration between clinicians, pharmacists and general practitioners as well as the use of computer-based medication prescription systems in hospitals will help to avoid and notice the frequently unnoticed drug-drug interactions.…”

Section: Conclusion and Future Prospectsmentioning

confidence: 99%

See 1 more Smart Citation

The use of liquid chromatography-tandem mass spectrometry for therapeutic drug monitoring of antibiotics in cancer patients

El‐Najjar

Gessner

2017

Clinical Chemistry and Laboratory Medicine (CCLM)

View full text Add to dashboard Cite

Cancer remains a leading cause of mortality and morbidity worldwide. In addition to organ failure, the most frequent reasons for admission of cancer patients to intensive care units (ICU) are: infections and sepsis. As critically ill, the complexity of the health situation of cancer patients renders the standard antimicrobial regimen more complex and even inadequate which results in increased mortality rates. This is due to pathophysiological changes in the volume of distribution, increased clearance, as well as to organ dysfunction. While in the former cases a decrease in drug efficacy is observed, the hallmark of the latter one is overdosing leading to increased toxicity at the expense of efficacy. Furthermore, an additional risk factor is the potential drug-drug interaction between antibiotics and antineoplastic agents. Therefore, therapeutic drug monitoring (TDM) is a necessity to improve the clinical outcome of antimicrobial therapy in cancer patients. To be applied in routine analysis the method used for TDM should be cheap, fast and highly accurate/sensitive. Furthermore, as ICU patients are treated with a cocktail of antibiotics the method has to cover the simultaneous analysis of antibiotics used as a first/second line of treatment. The aim of the current review is to briefly survey the pitfalls in the current antimicrobial therapy and the central role of TDM in dose adjustment and drug-drug interaction's evaluation. A major section is dedicated to summarize the currently published analytical methods and to shed light on the difficulties and potential problems that can be encountered during method development.

show abstract

UPLC–MS/MS method for therapeutic drug monitoring of 10 antibiotics used in intensive care units

El‐Najjar

Hösl

Holzmann

et al. 2017

Drug Testing and Analysis

View full text Add to dashboard Cite

A large variation in the levels of different ß-lactams and other antibiotics used in critically ill patients has been documented. The aim of this study is to establish and validate a fast, ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for the simultaneous analysis of ten antibiotics (Meropenem, Cefepime, Ceftazidime, Piperacillin, Benzylpenicillin, Ampicillin, Flucloxacillin, Linezolid, and Sulfamethoxazole/Trimethoprim) in human plasma according to European Medicines Agency (EMA) guidelines. Protein precipitation with ice-cold methanol containing 9 isotopically labeled internal standards was used for sample clean up. Antibiotics were detected, following a 4-minute gradient separation, in multiple reactions monitoring (MRM) using API 4000 instrument equipped with electrospray source operating in positive ion mode. The lower limit of quantification was 0.1 mg/L for Meropenem, Ceftazidime, Piperacillin, Ampicillin, Flucloxacillin, and Sulfamethoxazole; 0.05 mg/L for Cefepime, Benzylpenicillin, and Trimethoprim; and 0.02 mg/L for Linezolid. The method proved to be precise and accurate and applicable for therapeutic drug monitoring and other pharmacokinetic studies.

show abstract

Simultaneous quantification of antimicrobial agents for multidrug-resistant bacterial infections in human plasma by ultra-high-pressure liquid chromatography–tandem mass spectrometry

Cited by 78 publications

References 50 publications

An Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry Method for the Quantification of Vancomycin Requiring Only 2 µL of Rabbit Serum

An Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry Method for the Quantification of Vancomycin Requiring Only 2 µL of Rabbit Serum

The use of liquid chromatography-tandem mass spectrometry for therapeutic drug monitoring of antibiotics in cancer patients

UPLC–MS/MS method for therapeutic drug monitoring of 10 antibiotics used in intensive care units

Contact Info

Product

Resources

About

Simultaneous quantification of antimicrobial agents for multidrug-resistant bacterial infections in human plasma by ultra-high-pressure liquid chromatography–tandem mass spectrometry

Cited by 78 publications

References 50 publications

An Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry Method for the Quantification of Vancomycin Requiring Only 2 &#181;L of Rabbit Serum

An Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry Method for the Quantification of Vancomycin Requiring Only 2 &#181;L of Rabbit Serum

The use of liquid chromatography-tandem mass spectrometry for therapeutic drug monitoring of antibiotics in cancer patients

UPLC–MS/MS method for therapeutic drug monitoring of 10 antibiotics used in intensive care units

Contact Info

Product

Resources

About

An Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry Method for the Quantification of Vancomycin Requiring Only 2 µL of Rabbit Serum

An Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry Method for the Quantification of Vancomycin Requiring Only 2 µL of Rabbit Serum