PARP1 orchestrates variant histone exchange in signal‐mediated transcriptional activation

O’Donnell, Amanda; Yang, Shen Hsi; Sharrocks, Andrew D.

doi:10.1038/embor.2013.164

Cited by 19 publications

(14 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Epigenetic changes, such as histone post‐translational modifications, nucleosome rearrangement, and changes in the three‐dimensional architecture of the genome, play a crucial role in the precise regulation of gene expression . PARP‐1 orchestrates variant histone exchange to promote dynamic remodeling of nucleosomes, which effectively regulates transcription of genes in response to various cellular signals . PARP‐1 interacts broadly with nucleosomes and chromatin‐associated acceptor proteins, and these interactions allow PARP‐1 to localize to specific target genes to modify chromatin .…”

Section: Discussionmentioning

confidence: 99%

Melatonin regulates PARP1 to control the senescence‐associated secretory phenotype (SASP) in human fetal lung fibroblast cells

Wang

Geng

et al. 2017

Journal of Pineal Research

View full text Add to dashboard Cite

Cellular senescence is an important tumor-suppressive mechanism. However, acquisition of a senescence-associated secretory phenotype (SASP) in senescent cells has deleterious effects on the tissue microenvironment and, paradoxically, promotes tumor progression. In a drug screen, we identified melatonin as a novel SASP suppressor in human cells. Strikingly, melatonin blunts global SASP gene expression upon oncogene-induced senescence (OIS). Moreover, poly(ADP-ribose) polymerase-1 (PARP-1), a sensor of DNA damage, was identified as a new melatonin-dependent regulator of SASP gene induction upon OIS. Here, we report two different but potentially coherent epigenetic strategies for melatonin regulation of SASP. The interaction between the telomeric repeat-containing RNA (TERRA) and PARP-1 stimulates the SASP, which was attenuated by 67.9% (illustrated by the case of IL8) by treatment with melatonin. Through binding to macroH2A1.1, PARP-1 recruits CREB-binding protein (CBP) to mediate acetylation of H2BK120, which positively regulates the expression of target SASP genes, and this process is interrupted by melatonin. Consequently, the findings provide novel insight into melatonin's epigenetic role via modulating PARP-1 in suppression of SASP gene expression in OIS-induced senescent cells. Our studies identify melatonin as a novel anti-SASP molecule, define PARP-1 as a new target by which melatonin regulates SASP, and establish a new epigenetic paradigm for a pharmacological mechanism by which melatonin interrupts PARP-1 interaction with the telomeric long noncoding RNA(lncRNA) or chromatin.

show abstract

Section: Discussionmentioning

confidence: 99%

Melatonin regulates PARP1 to control the senescence‐associated secretory phenotype (SASP) in human fetal lung fibroblast cells

Wang

Geng

et al. 2017

Journal of Pineal Research

View full text Add to dashboard Cite

show abstract

“…Replacement of the canonical histones with the histone variants alters the biochemical and biophysical nature of the nucleosome by affecting PTMs, protein interactions or higher-order chromatin structure, thereby these may play a role affecting distinct cellular processes. For example, one of the most extensively studied histone variant H2A.Z, which is highly conserved and essential for many organisms, has been linked to a wide variety of different nuclear functions, including DNA replication and cell-cycle control [49], DNA damage repair [50], transcriptional activation and repression [51–53], maintenance of heterochromatin [54], anti-silencing [55], chromosome segregation [56], and genome integrity [57,58]. The diverse roles of histone variants in many major epigenetic processes lead to the expectation that dysregulation can result in pathological disorders, and indeed altered expression of variants ( i.e.…”

Section: Epigenetics In Health and Disease: Translational And Clinmentioning

confidence: 99%

Progress in epigenetic histone modification analysis by mass spectrometry for clinical investigations

Önder

Sidoli

Carroll³

et al. 2015

Expert Review of Proteomics

View full text Add to dashboard Cite

Chromatin biology and epigenetics are scientific fields in rapid expansion due to their fundamental role in understanding cell development, heritable characters and progression of diseases. Histone post-translational modifications (PTMs) are major regulators of the epigenetic machinery, due to their ability to modulate gene expression, DNA repair and chromosome condensation. Large- scale strategies based on mass spectrometry have been impressively improved in the last decade, so that global changes of histone PTM abundances are quantifiable with nearly routine proteomics analyses and it is now possible to determine combinatorial patterns of modifications. Presented here is an overview of the most utilized and newly developed proteomics strategies for histone PTM characterization and a number of case studies where epigenetic mechanisms have been comprehensively characterized. Moreover, a number of current epigenetics therapies are illustrated, with an emphasis on cancer

show abstract

“…H2A.Z was linked to both transcriptional activation and repression [ 17 ]. Genome-wide studies in a variety of organisms show that H2A.Z is enriched at the promoter of inducible genes under repressed or basal expression conditions, but is subsequently removed upon transcriptional activation [ 18 , 19 ]. A few recent studies further demonstrate that H2A.Z exhibits a repressive role in gene transcription [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Histone variants H2A.Z and H3.3 coordinately regulate PRC2-dependent H3K27me3 deposition and gene expression regulation in mES cells

Wang

Long

et al. 2018

BMC Biol

View full text Add to dashboard Cite

BackgroundThe hierarchical organization of eukaryotic chromatin plays a central role in gene regulation, by controlling the extent to which the transcription machinery can access DNA. The histone variants H3.3 and H2A.Z have recently been identified as key regulatory players in this process, but the underlying molecular mechanisms by which they permit or restrict gene expression remain unclear. Here, we investigated the regulatory function of H3.3 and H2A.Z on chromatin dynamics and Polycomb-mediated gene silencing.ResultsOur ChIP-seq analysis reveals that in mouse embryonic stem (mES) cells, H3K27me3 enrichment correlates strongly with H2A.Z. We further demonstrate that H2A.Z promotes PRC2 activity on H3K27 methylation through facilitating chromatin compaction both in vitro and in mES cells. In contrast, PRC2 activity is counteracted by H3.3 through impairing chromatin compaction. However, a subset of H3.3 may positively regulate PRC2-dependent H3K27 methylation via coordinating depositions of H2A.Z to developmental and signaling genes in mES cells. Using all-trans retinoic acid (tRA)-induced gene as a model, we show that the dynamic deposition of H2A.Z and H3.3 coordinately regulates the PRC2-dependent H3K27 methylation by modulating local chromatin structure at the promoter region during the process of turning genes off.ConclusionsOur study provides key insights into the mechanism of how histone variants H3.3 and H2A.Z function coordinately to finely tune the PRC2 enzymatic activity during gene silencing, through promoting or impairing chromosome compaction respectively.Electronic supplementary materialThe online version of this article (10.1186/s12915-018-0568-6) contains supplementary material, which is available to authorized users.

show abstract

PARP1 orchestrates variant histone exchange in signal‐mediated transcriptional activation

Abstract: PARP1 orchestrates variant histone exchange in signal-mediated transcriptional activationThis study reveals an essential role for PARP1 in the exchange of the histone variant H2A.Z by H2A at the -1 nucleosome of the FOS promoter, which promotes signal-induced transcriptional activation.

Cited by 19 publications

References 16 publications

Melatonin regulates PARP1 to control the senescence‐associated secretory phenotype (SASP) in human fetal lung fibroblast cells

Melatonin regulates PARP1 to control the senescence‐associated secretory phenotype (SASP) in human fetal lung fibroblast cells

Progress in epigenetic histone modification analysis by mass spectrometry for clinical investigations

Histone variants H2A.Z and H3.3 coordinately regulate PRC2-dependent H3K27me3 deposition and gene expression regulation in mES cells

Contact Info

Product

Resources

About