Investigating intratumour heterogeneity by single-cell sequencing

Ren, Shancheng; Qu, Min; Sun, Ying-hao

doi:10.1038/aja.2013.106

Cited by 18 publications

(13 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[ 3 ] who proposed the concept of branched evolutionary tumor growth. Our dynamic study in a living patient reinforces this concept and the clonal evolution theory [ 6 , 7 ]. However, the fact that we identified a minority TP53 -mutated clone in the primary tumor later expanded in the metastasis suggests that molecular abnormalities responsible for the metastatic process are usually not highlighted by whole tissue analyses of primary tumors.…”

Section: Discussionsupporting

confidence: 80%

See 1 more Smart Citation

Tracking sub-clonal TP53 mutated tumor cells in human metastatic renal cell carcinoma

et al. 2015

View full text Add to dashboard Cite

Renal Cell Carcinomas (RCCs) are heterogeneous tumors with late acquisition of TP53 abnormalities during their evolution. They harbor TP53 abnormalities in their metastases. We aimed to study TP53 gene alterations in tissue samples from primary and metastatic RCCs in 36 patients followed up over a median of 4.2 years, and in xenografted issued from primary RCCs.In 36 primary RCCs systematically xenografted in mice, and in biopsies of metastases performed whenever possible during patient follow-up, we studied p53-expressing tumor cells and TP53 gene abnormalities.We identified TP53 gene alterations in primary tumors, metastases and xenografts.Quantification of tumors cells with TP53 gene alterations showed a significant increase in the metastases compared to the primary RCCs, and, strikingly, the xenografts were similar to the metastases and not to the primary RCCs from which they were derived.Using laser-microdissection of p53-expressing tumor cells, we identified TP53-mutated tumor cells in the xenografts derived from the primary RCC, and in a lung metastasis later developed in one patient. The mutation enabled us to track back their origin to a minority sub-clone in the primary heterogeneous RCC.Combining in situ and molecular analyses, we demonstrated a clonal expansion in a living patient with metastatic RCC.

show abstract

Section: Discussionsupporting

confidence: 80%

“…According to the clonal evolution model, oncogenic selection increases the phenotypic and genotypic heterogeneity of cancer cells within tumors [ 6 , 7 ]. A malignant tumor is composed of sub-clones, and additional mutations can occur during evolution [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Tracking sub-clonal TP53 mutated tumor cells in human metastatic renal cell carcinoma

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Single-cell genomic sequencing or single nuclear genome sequencing is useful to study mutations, single nucleotide variations, and indels (insertion and deletions) [ 50 ] . Multiple methods of SCS for single nuclear genome have been designed [ Table 1 ].…”

Section: Single-cell Sequencing: a Promising Tool For Deciphering Tummentioning

confidence: 99%

Centrosome aberrations and chromosome instability contribute to tumorigenesis and intra-tumor heterogeneity

Jusino¹,

Fernández-Padín²,

Saavedra³

2018

JCMT

View full text Add to dashboard Cite

Centrosomes serve as the major microtubule organizing centers in cells and thereby contribute to cell shape, polarity, and motility. Also, centrosomes ensure equal chromosome segregation during mitosis. Centrosome aberrations arise when the centrosome cycle is deregulated, or as a result of cytokinesis failure. A long-standing postulate is that centrosome aberrations are involved in the initiation and progression of cancer. However, this notion has been a subject of controversy because until recently the relationship has been correlative. Recently, it was shown that numerical or structural centrosome aberrations can initiate tumors in certain tissues in mice, as well as invasion. Particularly, we will focus on centrosome amplification and chromosome instability as drivers of intra-tumor heterogeneity and their consequences in cancer. We will also discuss briefly the controversies surrounding this theory to highlight the fact that the role of both centrosome amplification and chromosome instability in cancer is highly context-dependent. Further, we will discuss single-cell sequencing as a novel technique to understand intra-tumor heterogeneity and some therapeutic approaches to target chromosome instability.

show abstract

“…Prostate cancer is a kind of heterogeneous cancer with different phenotypes and outcomes 81. Most PCa present to be indolent with mild and atypical clinical manifestations, which may confuse and mislead clinicians and patients at the point of disease diagnosis.…”

Section: Conclusion and Prospectivementioning

confidence: 99%

Current early diagnostic biomarkers of prostate cancer

Qu¹,

Ren²,

Sun³

2014

Asian J Androl

Self Cite

View full text Add to dashboard Cite

Prostate cancer (PCa) has become to have the highest incidence and the second mortality rate in western countries, affecting men's health to a large extent. Although prostate-specific antigen (PSA) was discovered to help diagnose the cancer in an early stage for decades, its specificity is relative low, resulting in unnecessary biopsy for healthy people and over-treatment for patients. Thus, it is imperative to identify more and more effective biomarkers for early diagnosis of PCa in order to distinguish patients from healthy populations, which helps guide an early treatment to lower disease-related mortality by noninvasive or minimal invasive approaches. This review generally describes the current early diagnostic biomarkers of PCa in addition to PSA and summarizes the advantages and disadvantages of these biomarkers.

show abstract

Investigating intratumour heterogeneity by single-cell sequencing

Cited by 18 publications

References 45 publications

Tracking sub-clonal TP53 mutated tumor cells in human metastatic renal cell carcinoma

Tracking sub-clonal TP53 mutated tumor cells in human metastatic renal cell carcinoma

Centrosome aberrations and chromosome instability contribute to tumorigenesis and intra-tumor heterogeneity

Current early diagnostic biomarkers of prostate cancer

Contact Info

Product

Resources

About