Abstract:We achieved continuous, noncontact wide-field imaging and characterization of drug release from a polymeric device in vitro by uniquely using off-axis interferometric imaging. Unlike the current gold-standard methods in this field, which are usually based on chromatography and spectroscopy, our method requires no user intervention during the experiment and involves less lab consumable instruments. Using a simplified interferometric imaging system, we experimentally demonstrate the characterization of anestheti… Show more
“…[ 20 ] Holographic recording and replaying of waves have been used in numerous applications such as wavefront shaping, [ 21,22 ] strain‐free observations of biological cells, [ 16,23 ] optical metrology of nanostructures and phase profiling of chemical processes. [ 24,25 ]…”
Section: Introductionmentioning
confidence: 99%
“…[20] Holographic recording and replaying of waves have been used in numerous applications such as wavefront shaping, [21,22] strain-free observations of biological cells, [16,23] optical metrology of nanostructures and phase profiling of chemical processes. [24,25] There are two main configurations for holographic wavefront acquisition: in-line and off-axis digital holography (DH). In in-line DH, the sample beam and reference beam interfere with no angle between each other, producing a hologram that contains concentric circular interference fringes.…”
Modern quantitative optical imaging is developing toward high throughput and powerful data processing capabilities. Holography is a powerful technique to characterize quantitative phase delays introduced by light–matter interactions. The spatial bandwidth utilization of imaging sensors in digital holography can be expanded via an off‐axis multiplexing technique, which is a powerful tool for high‐throughput quantitative optical imaging. However, the highest bandwidth utilization of sensor is limited at 58.9% to keep the signal spectra away from the zeroth spectra. Kramers–Kronig relation is introduced into the off‐axis multiplexing technology to allow for the overlapping between the signal spectra and unwanted spectra. The bandwidth utilization of sensor in a diffraction‐limited optical system can reach 78.5% in one hologram. It opens a new route to multiplexing quantitative optical imaging and helps to improve the performance of iterative‐free and constraint‐free modern optical microscopes in various spectral regimes.
“…[ 20 ] Holographic recording and replaying of waves have been used in numerous applications such as wavefront shaping, [ 21,22 ] strain‐free observations of biological cells, [ 16,23 ] optical metrology of nanostructures and phase profiling of chemical processes. [ 24,25 ]…”
Section: Introductionmentioning
confidence: 99%
“…[20] Holographic recording and replaying of waves have been used in numerous applications such as wavefront shaping, [21,22] strain-free observations of biological cells, [16,23] optical metrology of nanostructures and phase profiling of chemical processes. [24,25] There are two main configurations for holographic wavefront acquisition: in-line and off-axis digital holography (DH). In in-line DH, the sample beam and reference beam interfere with no angle between each other, producing a hologram that contains concentric circular interference fringes.…”
Modern quantitative optical imaging is developing toward high throughput and powerful data processing capabilities. Holography is a powerful technique to characterize quantitative phase delays introduced by light–matter interactions. The spatial bandwidth utilization of imaging sensors in digital holography can be expanded via an off‐axis multiplexing technique, which is a powerful tool for high‐throughput quantitative optical imaging. However, the highest bandwidth utilization of sensor is limited at 58.9% to keep the signal spectra away from the zeroth spectra. Kramers–Kronig relation is introduced into the off‐axis multiplexing technology to allow for the overlapping between the signal spectra and unwanted spectra. The bandwidth utilization of sensor in a diffraction‐limited optical system can reach 78.5% in one hologram. It opens a new route to multiplexing quantitative optical imaging and helps to improve the performance of iterative‐free and constraint‐free modern optical microscopes in various spectral regimes.
“…Digital Holographic (DH) as an effective technology can record complete wavefront information by the principle of interferometry, it can complete real-time high-robust phase recovery with a single-shot hologram. DH has many significant applications such as living cell analysis 1 , drug release monitoring in vitro 2 , and optical metrology of nanostructures 3 .…”
Selecting a suitable autofocus critical function to accurately obtain the defocused distance in a digital holographic microscope (DHM) is the key to digital reconstruction. Several typical autofocus methods have been proposed to calculate the defocused distance by ascertaining the global extreme value of the critical functions to determine the best focused plane. However, these typical methods are easily affected by noise, or exhibit parameter dependence. In this work, a novel digital holographic autofocus algorithm based on the weighting factor of the discrete cosine transform (DCT) is proposed. We compare the proposed method with several typical methods involving SPEC, DIF, GRA and LAP to evaluate the capability of our method under noise-free condition and different noise conditions. Futhermore, to avoid artificially selecting the filter window and to find the optimal interval where the extreme value is located, we filter the high-frequency components in the form of percentages during the process of bandpass-filtering. The experimental results of HeLa cells and phase resolution target verify that our method has a good performance in terms of applicability and noise-resistance.
“…Temporal-phase stability is identified as one of the most important figures of merit in QPI. 6 Among all DHM configurations, common-path DHM systems 7 – 16 provide the highest temporal stability, allowing subnanometer path-length temporal sensitivity to study dynamic events in live biological specimens. High-speed image acquisition is also critical in capturing rapid dynamic events and investigating how they are affected by external perturbations.…”
Significance: The hallmarks of digital holographic microscopy (DHM) compared with other quantitative phase imaging (QPI) methods are high speed, accuracy, spatial resolution, temporal stability, and polarization-sensitivity (PS) capability. The above features make DHM suitable for real-time quantitative PS phase imaging in a broad number of biological applications aimed at understanding cell growth and dynamic changes occurring during physiological processes and/or in response to pharmaceutical agents. Aim: The insertion of a Fresnel biprism (FB) in the image space of a light microscope potentially turns any commercial system into a DHM system enabling QPI with the five desired features in QPI simultaneously: high temporal sensitivity, high speed, high accuracy, high spatial resolution, and PS. To the best of our knowledge, this is the first FB-based DHM system providing these five features all together. Approach: The performance of the proposed system was calibrated with a benchmark phase object. The PS capability has been verified by imaging human U87 glioblastoma cells. Results: The proposed FB-based DHM system provides accurate phase images with high spatial resolution. The temporal stability of our system is in the order of a few nanometers, enabling live-cell studies. Finally, the distinctive behavior of the cells at different polarization angles (e.g., PS capability) can be observed with our system. Conclusions: We have presented a method to turn any commercial light microscope with monochromatic illumination into a PS QPI system. The proposed system provides accurate quantitative PS phase images in a new, simple, compact, and cost-effective format, thanks to the low cost (a few hundred dollars) involved in implementing this simple architecture, enabling the use of this QPI technique accessible to most laboratories with standard light microscopes.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.