An integrated exact mass spectrometric strategy for comprehensive and rapid characterization of phenolic compounds in licorice

Abstract

The LC/MS analysis for each injection took less than 9 min. The developed method is fast, accurate and reliable due to its high resolution and high efficiency characteristics as a result of combining both UPLC separation and QTOF exact mass measurement.

Characterization of phenolic compounds and antioxidant properties of Glycyrrhiza glabra L. rhizomes and roots

“…Many papers have been published proling phenolic compounds in G. glabra samples from different origins and using different extraction methodologies, some of them cited in the previous discussion. 34,35,[37][38][39][40][41][42][43][44][45][46] However, from all of them, only Montoro et al (2011) presented quantitative results, although they cannot be compared with ours results since they are expressed differently (mg g À1 of dry plant), thus these authors revealed liquiritin apioside as the main avonoid present in their sample, which is in agreement with the sample studied herein. In our case, the results were expressed in mg g À1 of extract in order to relate the amounts of phenolic compounds found in the extract to the antioxidant activity.…”

Chemical profiling and quantification of Gua-Lou-Gui-Zhi decoction by high performance liquid chromatography/quadrupole-time-of-flight mass spectrometry and ultra-performance liquid chromatography/triple quadrupole mass spectrometry

“…The negative ion mode was much more suitable for the analysis of this kind of compounds. Except for methyl gallate (21), ethyl gallate (32), protocatechuic aldehyde (18) and succinic acid (4), other 13 phenolic acids including gallic acid (8), protocatechuic acid (13), neochlorogenic acid (16), chlorogenic acid (19), p-hydroxybenzoic acid (20), vanillic acid (24), 2-methoxycinnamic acid (25), caffeic acid (27), 4-hydroxycinnamic acid (35), ferulic Acid (39), 3-hydroxycinnamic acid (45), 2-hydroxycinnamic acid (54) and cinnamic acid (65) had one carboxylic group in their structures, and their characteristic fragmentation behavior was the loss of CO 2 (44 Da) (take cinnamic acid as an example, Fig. 3C).…”

“…These compounds produced two diagnostic fragment [28,29,[35][36][37]. Chalcones with C-2 -OH could transform to corresponding flavanone isomers, and then produced the same typical fragment ions via Y 0 and RDA reaction [28,35]. [35,36].…”

“…Rutin (42), quercetin-7-O-glucopyranoside (43), isoquercitrin (55), astragalin (56) and luteoloside (64) have similar fragmentation pathways to produce diagnostic fragment Y 0 (loss of Glc or rutinose group (Glc 6 -1 Rha)), suggesting these compounds are O-glycosyl flavonoids. These compounds produced two diagnostic fragment [28,29,[35][36][37]. Chalcones with C-2 -OH could transform to corresponding flavanone isomers, and then produced the same typical fragment ions via Y 0 and RDA reaction [28,35].…”

“…Chalcones with C-2 -OH could transform to corresponding flavanone isomers, and then produced the same typical fragment ions via Y 0 and RDA reaction [28,35]. [35,36]. Compounds 62, 95 and 103 had one methyl in their structures which characteristic fragmentation behavior was the loss of CH 3 (15 Da), and yielded fragment ions via RDA reaction (A 1,3 , B 1,3 ), So they were deduced as licochalcone B, glycyrrhisoflavanone and licochalcone A, respectively [28,29,35,37].…”