Biological properties of carotenoids extracted from Halobacterium halobium isolated from a Tunisian solar saltern

Abbes, Molka; Baati, Houda; Guermazi, Sonda; Messina, Concetta María; Santulli, Andrea; Gharsallah, Néji; Ammar, Emna

doi:10.1186/1472-6882-13-255

Cited by 87 publications

(72 citation statements)

References 46 publications

(47 reference statements)

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“…EP3 could tentatively be identified as a derivative of bacterioruberin. Data reported in the literature provide support that bacterioruberin is a characteristic carotenoid from halophilic microorganisms (Abbes et al ., ), and the spectral peaks characteristic of red carotenoid exhibit maximal absorption at 467, 493 and 527 nm (Britton, ), similar to what we observed in this study. The main type of carotenoid produced by Planococcus species has been described as Glyco‐C30‐carotenoic acid.…”

Section: Discussionsupporting

confidence: 90%

Factors influencing pigment production by halophilic bacteria and its effect on brine evaporation rates

Silva-Castro

Moyo

Khumalo

et al. 2018

Microbial Biotechnology

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Summary The disposal of reject brine, a highly concentrated waste by‐product generated by various industrial processes, represents a major economic and environmental challenge. The common practice in dealing with the large amounts of brine generated is to dispose of it in a pond and allow it to evaporate. The rate of evaporation is therefore a key factor in the effectiveness of the management of these ponds. The addition of various dyes has previously been used as a method to increase the evaporation rate. In this study, a biological approach, using pigmented halophilic bacteria (as opposed to chemical dyes), was assessed. Two bacteria, an Arthrobacter sp. and a Planococcus sp. were selected due to their ability to increase the evaporation of synthetic brine. When using industrial brine, supplementation of the brine with an iron source was required to maintain the pigment production. Under these conditions, the Planococcus sp. CP 5‐4 produced a carotenoid‐like pigment, which resulted in a 20% increase in the evaporation rate of the brine. Thus, the pigment production capability of halophilic bacteria could potentially be exploited as an effective step in the management of industrial reject brines, analogous to the crystallizer ponds used to mine salt from sea water.

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Section: Discussionsupporting

confidence: 90%

Factors influencing pigment production by halophilic bacteria and its effect on brine evaporation rates

Silva-Castro

Moyo

Khumalo

et al. 2018

Microbial Biotechnology

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“…Each well was exposed to 10 μl of DCF-DA in HBSS (5 μg/ml), incubated for 5 min at 37 °C to allow the oxidation of the DCF-DA and successively read on a spectrofluorometer, 485 exc - 530 em (Varian Cary Eclipse; Agilent Technologies, Santa Clara, CA, USA). To further support the presence of ROS, additional wells were treated with the antioxidant N-acetilcysteine that inhibits its formation, according to a standardized protocol [32]. The results were expressed as relative fluorescence/μg of total proteins (rf/μg tp).…”

Section: Methodsmentioning

confidence: 99%

Anisakis pegreffii (Nematoda: Anisakidae) products modulate oxidative stress and apoptosis-related biomarkers in human cell lines

et al. 2016

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BackgroundIn countries with elevated prevalence of zoonotic anisakiasis and high awareness of this parasitosis, a considerable number of cases that associate Anisakis sp. (Nematoda, Anisakidae) and different bowel carcinomas have been described. Although neoplasia and embedded larvae were observed sharing the common site affected by chronic inflammation, no association between the nematode and malignancy were directly proved. Similarly, no data are available about the effect of secretory and excretory products of infecting larvae at the host’s cellular level, except in respect to allergenic interaction.MethodsTo test the mechanisms by which human non-immune cells respond to the larvae, we exposed the fibroblast cell line HS-68 to two Anisakis products (ES, excretory/secretory products; and EC, crude extract) and evaluated molecular markers related to stress response, oxidative stress, inflammation and apoptosis, such as p53, HSP70, TNF-α, c-jun and c-fos, employing cell viability assay, spectrophotometry, immunoblotting and qPCR.ResultsBoth Anisakis products led to increased production of reactive oxygen species (ROS), especially in EC-treated cells. While the ES treatment induces activation of kinases suggesting inflammation and cell proliferation (or inhibition of apoptosis), in EC-treated cells, other signaling pathways indicate the inhibition of apoptosis, marked by strong upregulation of Hsp70. Elevated induction of p53 in fibroblasts treated by both Anisakis products, suggests a significantly negative effect on the host DNA.ConclusionsThis study shows that in vitro cell response to Anisakis products can result in at least two different scenarios, which in both cases lead to inflammation and DNA damage. Although these preliminary results are far from proving a relationship between the parasite and cancer, they are the first to support the existence of conditions where such changes are feasible.Electronic supplementary materialThe online version of this article (doi:10.1186/s13071-016-1895-5) contains supplementary material, which is available to authorized users.

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“…The total pigment content present was estimated by measuring the absorbance of the culture broth at 490 nm ( λ max of haloarchaeal carotenoid) using UV–Vis Spectrophotometer (UV-1800, Shimadzu, Japan) (Abbes et al 2013). …”

Section: Methodsmentioning

confidence: 99%

Response of extreme haloarchaeon Haloarcula argentinensis RR10 to simulated microgravity in clinorotation

Thombre¹,

Shinde²,

Dixit

et al. 2017

3 Biotech

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Gravity is the fundamental force that may have operated during the evolution of life on Earth. It is thus important to understand as to what the effects of gravity are on cellular life. The studies related to effect of microgravity on cells may provide greater insights in understanding of how the physical force of gravity shaped life on Earth. The present study focuses on a unique group of organisms called the Haloarchaea, which are known for their extreme resistance to survive in stress-induced environments. The aim of the present investigation was to study the effect of simulated microgravity on physiological response of extremely halophilic archaeon, Haloarcula argentinensis RR10, under slow clinorotation. The growth kinetics of the archaeon in microgravity was studied using the Baryani model and the viable and apoptotic cells were assessed using propidium iodide fluorescent microscopic studies. The physiological mechanism of adaptation was activation of ‘salt-in’ strategy by intracellular sequestration of sodium ions as detected by EDAX. The organism upregulated the production of ribosomal proteins in simulated microgravity as evidenced by Matrix-assisted laser desorption ionization Time of flight–Mass Spectrophotometry. Simulated microgravity altered the antibiotic susceptibility of the haloarchaeon and it developed resistance to Augmentin, Norfloxacin, Tobramycin and Cefoperazone, rendering it a multidrug resistant strain. The presence of antibiotic efflux pump was detected in the haloarchaeon and it also enhanced production of protective carotenoid pigment in simulated microgravity. The present study is presumably the first report of physiological response of H. argentinensis RR10 in microgravity simulated under slow clinorotation.

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Biological properties of carotenoids extracted from Halobacterium halobium isolated from a Tunisian solar saltern

Cited by 87 publications

References 46 publications

Factors influencing pigment production by halophilic bacteria and its effect on brine evaporation rates

Factors influencing pigment production by halophilic bacteria and its effect on brine evaporation rates

Anisakis pegreffii (Nematoda: Anisakidae) products modulate oxidative stress and apoptosis-related biomarkers in human cell lines

Response of extreme haloarchaeon Haloarcula argentinensis RR10 to simulated microgravity in clinorotation

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