Anti-<i>Helicobacter pylori</i> and Antiulcerogenic Activities of the Root Cortex of <i>Paeonia suffruticosa</i>

Abstract

In this study, we evaluated the gastric protective activities of mokdanpi in vitro. Further, we used experimental ulcer models to identify the active ingredients of mokdanpi. As a preliminary evaluation of mokdanpi ethanolic extract and its ingredients, we assessed its radical scavenging activities. In addition, its antimicrobial activity against Helicobacter pylori (H. pylori) was investigated. The antiulcerogenic activity of the active ingredients was evaluated in pylorus-ligated rats, an HCl/ethanol-induced and an absolute ethanol-induced ulcer model. We confirmed the scavenging effect of the ethanolic extract of mokdanpi and its ingredients against 2,2-diphenyl-1-picrylhydrazyl, nitric oxide and superoxide radicals, and we demonstrated that mokdanpi could inhibit the colonization of H. pylori. In an HCl-ethanol-induced ulcer model, gallic acid and catechin (100 mg/kg) inhibited 40.6% and 41.7% of gastric lesions, respectively. Catechin (100 mg/kg) significantly reduced (p<0.05) the gastric secretion induced by pylorus ligature in rats in comparison to the control group. Gallic acid (100 mg/kg) significantly increased (p<0.05) the mucus contents in an ethanol-induced ulcer model. The antioxidant ingredients (catechin and gallic acid) present in mokdanpi play a major role in antiulcerogenic activity, and demonstrate novel activity against H. pylori. Key words Paeonia suffruticosa; Helicobactor pylori; gastric ulcer; gastric cancer; catechin; gallic acidA large portion of the world's population is afflicted with gastric diseases, such as gastric ulcer, gastritis, and gastric cancer. These diseases are induced by stress, smoking, infection by Helicobacter pylori (H. pylori), and nutritional deficiencies.1) Gastric ulcers result from an imbalance between aggressive factors (i.e., gastric acid, pepsin, stimulation of the vagus nerves, secretion of gastrin, and an increased number of parietal cells) and protective factors (i.e., bicarbonate ion, mucus productivity, mucus secretion, and prostaglandins). 2)In particular, frequent gastritis or gastric ulcer is chronic and leads to death. Another important risk factor is infection from H. pylori. Since reports indicate that H. pylori-induced gastritis leads to gastric cancer, the control of H. pylori in the stomach has become a target of treatment for gastric diseases. The antibodies for H. pylori are higher in patients with gastric cancer than in control groups.3) For eradication of H. pylori, a combination therapy with a proton pump inhibitor (PPI) or bismuth and antibiotics has been used. Nevertheless, the infection rate remains high and new therapeutic alternatives are needed because of antimicrobial resistance. In this regard, plant-derived extracts have been considered in the development of antiulcerogenic drugs.Mokdanpi, the root cortex of Paeonia suffruticosa ANDREWS (Panunculaceae), is an important crude drug with analgesic, sedative, and anti-inflammatory activities, and has been used as a remedy for cardiovascular extravasated blood and female genital diseas...

Inhibitory Activities of Palmatine from Coptis chinensis Against Helicobactor pylori and Gastric Damage

“…H. pylori strain (ATCC 43504) was obtained from ATCC (Rockville, MD, USA). Anti- H. pylori activity was examined according to a previous study (11). Briefly, brucella agar medium containing 7% horse serum (7 ml) was added to each sample (1 ml).…”

Development of a Fast, Low‐Cost, Conservative and Ecological Method for Quantifying Gallic Acid in Polymeric Formulations by FTIR Spectroscopy in Solution

“…GA attracts the interest of researchers mainly for its antioxidant capacity and ability in counteracting oxidative stress (OS) and correlated diseases as cancer, HIV, ulcer, inflammation, bacterial infections, fungi infections and neurodegenerative disorders …”

Atividade antiulcerogênica do extrato aquoso de Salvia officinalis L. (Lamiaceae)

“…Dessa forma, as ulcerações induzidas não são inibidas por agentes que interferem na secreção de ácido, mas, por substâncias que aumentam os fatores de defesa da mucosa, como por exemplo, os antioxidantes e as prostaglandinas (Miller et al, 1979). As lesões induzidas na mucosa estomacal podem estar relacionadas à produção excessiva de radicais livres e aumento do conteúdo de peróxidos lipídicos derivados de oxigênio resultando em alterações significativas em nível celular causando danos às membranas, morte celular e erosão epitelial (La Casa et al, 2000;Birdane et al, 2007;Li et al, 2008Jung et al, 2013.…”

“…O etanol especificamente provoca lesões necróticas na mucosa gástrica através de vários mecanismos que reduzem os fatores de defesa: a secreção de bicarbonato e a produção de muco (Marhuenda et al, 1993). Atualmente têm-se considerado que as lesões agudas da mucosa podem evoluir para uma injúria do endotélio vascular, provocando uma diminuição do fluxo sanguíneo, a redução do fornecimento de oxigênio e consequentemente o transporte de nutrientes (Jung et al, 2013). Para o tratamento das lesões gástricas são utilizados os antibióticos (quando constatada a presença de Helycobacter pylori), anti-histamínicos (H 2 ), antiácidos e inibidores de bomba de prótons, porém, estes fármacos apresentam reações adversas como hipersensibilidade, arritmia e impotência (Braz et al, 2013), além de causarem resistência antimicrobiana (Batran et al, 2013).…”