Auxin and cytokinin control formation of the quiescent centre in the adventitious root apex of arabidopsis

Rovere, Federica Della; Fattorini, Lorenzo; D’Angeli, Simone; Veloccia, A; Falasca, Giuseppina; Altamura, Maria Maddalena

doi:10.1093/aob/mct215

Cited by 165 publications

(214 citation statements)

References 51 publications

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“…Tissues are continuously formed by asymmetric division of initial cells, which are stem cells, followed by proliferative divisions of their daughter meristematic cells. Stem cell activity is maintained by a quiescent center (QC; van den Berg et al, 1997;Drisch and Stahl, 2015) and auxin activity (Della Rovere et al, 2013). Auxin accumulation in the QC area triggers a dosedependent and slow response that activates PLETH-ORA (PLT) factors.…”

mentioning

confidence: 99%

Regulation of Hormonal Control, Cell Reprogramming, and Patterning during De Novo Root Organogenesis

et al. 2017

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Body regeneration through formation of new organs is a major question in developmental biology. We investigated de novo root formation using whole leaves of Arabidopsis (Arabidopsis thaliana). Our results show that local cytokinin biosynthesis and auxin biosynthesis in the leaf blade followed by auxin long-distance transport to the petiole leads to proliferation of J0121-marked xylem-associated tissues and others through signaling of INDOLE-3-ACETIC ACID INDUCIBLE28 (IAA28), CRANE (IAA18), WOODEN LEG, and ARABIDOPSIS RESPONSE REGULATORS1 (ARR1), ARR10, and ARR12. Vasculature proliferation also involves the cell cycle regulator KIP-RELATED PROTEIN2 and ABERRANT LATERAL ROOT FORMATION4, resulting in a mass of cells with rooting competence that resembles callus formation. Endogenous callus formation precedes specification of postembryonic root founder cells, from which roots are initiated through the activity of SHORT-ROOT, PLETHORA1 (PLT1), and PLT2. Primordia initiation is blocked in shr plt1 plt2 mutant. Stem cell regulators SCHIZORIZA, JACKDAW, BLUEJAY, and SCARECROW also participate in root initiation and are required to pattern the new organ, as mutants show disorganized and reduced number of layers and tissue initials resulting in reduced rooting. Our work provides an organ regeneration model through de novo root formation, stating key stages and the primary pathways involved.Plants have striking regeneration capacities, and can produce new organs from postembryonic tissues (Hartmann et al., 2010;Chen et al., 2014;Liu et al., 2014) as well as reconstitute damaged organs upon wounding (Xu et al., 2006;Heyman et al., 2013; PerianezRodriguez et al., 2014;Melnyk et al., 2015;Efroni et al., 2016). Intriguingly, root regeneration upon stem cell damage recruits embryonic pathways (Hayashi et al., 2006;Efroni et al., 2016), whereas in contrast, postembryonic formation of whole new organs, such as lateral roots, appears to use specific postembryonic pathways (Lavenus et al., 2013).Cross talk between auxin and cytokinin signaling is required for many aspects of plant development and regeneration (El-Showk et al., 2013), although how their synergistic interaction is implemented at the molecular level has not been clarified (Skoog and Miller, 1957;Chandler and Werr, 2015). Exogenous in vitro supplementation of these two hormones results in continuous cell proliferation, to form a characteristic structure termed "callus". Callus emerges as a common regenerative mechanism for almost all plant organs through in vitro culture (Atta et al., 2009;Sugimoto et al., 2010). There is increasing evidence that callus formation requires hormone-mediated activation of a lateral and meristematic root development program in pericycle-like cells defined by expression of the J0121 marker 1 This work was supported by grants from Ministerio de Economía y Competitividad (MINECO) of Spain, the European Regional Development Fund (ERDF) and FP7 Funds of the European Commission, BFU2013-41160-P, BFU2016-80315-P, and PCIG11-GA-2012-322082 to M.A....

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Regulation of Hormonal Control, Cell Reprogramming, and Patterning during De Novo Root Organogenesis

et al. 2017

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“…Despite their distinct regulation, in both cases the visible stages of adventitious root formation follow an equivalent pattern to lateral roots, including pericycle asymmetric cell divisions ( Fig. 3; Falasca and Altamura, 2003;Della Rovere et al, 2013).…”

Section: Adventitious Rooting In Arabidopsismentioning

confidence: 98%

“…ARF6, ARF8, and ARF17 oppositely regulate jasmonic acid homeostasis via regulating jasmonic acid-modifying GRETCHEN HAGEN3 (GH3) enzymes (Gutierrez et al, 2009(Gutierrez et al, , 2012Bellini et al, 2014). Auxin transporters are also important for adventitious root formation, with both influx (LAX3) and efflux (PIN1) carriers required for auxin accumulation in the early stages of adventitious root organogenesis (Della Rovere et al, 2013). An ATP-binding cassette transporter mediating IAA efflux (ABCB19) has also been linked to adventitious root formation (Sukumar et al, 2013).…”

Section: Adventitious Rooting In Arabidopsismentioning

confidence: 99%

Branching Out in Roots: Uncovering Form, Function, and Regulation

et al. 2014

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Root branching is critical for plants to secure anchorage and ensure the supply of water, minerals, and nutrients. To date, research on root branching has focused on lateral root development in young seedlings. However, many other programs of postembryonic root organogenesis exist in angiosperms. In cereal crops, the majority of the mature root system is composed of several classes of adventitious roots that include crown roots and brace roots. In this Update, we initially describe the diversity of postembryonic root forms. Next, we review recent advances in our understanding of the genes, signals, and mechanisms regulating lateral root and adventitious root branching in the plant models Arabidopsis (Arabidopsis thaliana), maize (Zea mays), and rice (Oryza sativa). While many common signals, regulatory components, and mechanisms have been identified that control the initiation, morphogenesis, and emergence of new lateral and adventitious root organs, much more remains to be done. We conclude by discussing the challenges and opportunities facing root branching research.

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“…TCLs have been used for the effective tissue culture of several dozen plants with commercial importance, spanning model plants such as orchids (Teixeira da Silva 2013), tobacco, Arabidopsis thaliana (Della Rovere et al 2013 or snapdragon, field crops (cereals, rape, rice) horticultural commodities (fruits, vegetables, ornamental plants), medicinal plants and herbs, and even forestry trees (Pinus sp. ), and woody fruit plants, Citrus spp.…”

Section: Tcls: a Brief Backgroundmentioning

confidence: 99%

“…The endodermis periclinal derivatives either organize root meristemoids, further developing into roots (i.e., a rhizogenic response), or proliferate into superimposed callus-like layers with xylary clumps or strands (xylogenesis) (Fig. 2B) (Della Rovere et al 2013. By using numerous mutants and transgenic lines it has been demonstrated that Arabidopsis plants form adventitious roots in planta starting from the pericycle of the hypocotyl, with no involvement of the hypocotyl's endodermis.…”

Section: Tcls: Actual and Potential Usesmentioning

confidence: 99%

The Untapped Potential Of Plant Thin Cell Layers

Silva¹,

Altamura

Dobránszki

2015

Journal of Horticultural Research

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Thin cell layers (TCLs), which contain a small number of cells or tissues, are explants excised from different organs (stems, leaves, roots, inflorescences, flowers, cotyledons, hypocotyls/epicotyls, and embryos). After almost 45 years of research, this culture system has been used for several monocotyledonous and dicotyledonous plants of commercial importance, and for model plants. The limited amount of cells in a TCL is of paramount importance because marker molecules/genes of differentiation can be easily localized in situ in the target/responsive cells. Thus, the use of TCLs has allowed, and continues to allow, for the expansion of knowledge in plant research in a practical and applied manner into the fields of tissue culture and micropropagation, cell and organ genetics, molecular biology, biochemistry, and development. Starting from a brief historical background, the actual and potential uses of the TCL system are briefly reviewed.

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Auxin and cytokinin control formation of the quiescent centre in the adventitious root apex of arabidopsis

Cited by 165 publications

References 51 publications

Regulation of Hormonal Control, Cell Reprogramming, and Patterning during De Novo Root Organogenesis

Regulation of Hormonal Control, Cell Reprogramming, and Patterning during De Novo Root Organogenesis

Branching Out in Roots: Uncovering Form, Function, and Regulation

The Untapped Potential Of Plant Thin Cell Layers

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