Continuous production of β-cyclodextrin from starch by highly stable cyclodextrin glycosyltransferase immobilized on chitosan

Abstract

Cyclodextrin glycosyltransferase (CGTase) from Thermoanaerobacter sp. was covalently immobilized on glutaraldehyde-activated chitosan spheres and used in a packed bed reactor to investigate the continuous production of β-cyclodextrin (β-CD). The optimum temperatures were 75 °C and 85 °C at pH 6.0, respectively for free and immobilized CGTase, and the optimum pH (5.0) was the same for both at 60 °C. In the reactor, the effects of flow rate and substrate concentration in the β-CD production were evaluated. The optimum substrate concentration was 4% (w/v), maximizing the β-CD production (1.32 g/L) in a flow rate of 3 mL/min. In addition, the biocatalyst had good operational stability at 60 °C, maintaining 61% of its initial activity after 100 cycles of batch and 100% after 100 h of continuous use. These results suggest the possibility of using this immobilized biocatalyst in continuous production of CDs.

Biomass derived β-cyclodextrin-SO 3 H carbonaceous solid acid catalyst for catalytic conversion of carbohydrates to 5-hydroxymethylfurfural

“…Intrigued by these studies [24,25] and our continuing work in this field [26,27], we choose readily available -cyclodextrin as a carbon precursor and p-TSA as a sulphonating agent. Naturally occurring -cyclodextrin can be prepared by enzymatic hydrolysis of starch which comes from biomass [28].…”

Mutations at calcium binding site III in cyclodextrin glycosyltransferase improve β-cyclodextrin specificity

“…Cyclodextrin glycosyltransferase (CGTase, EC 2.4.1.19) belongs to glycosyl hydrolases family 13, which includes many enzymes that catalyze transglycosylation using similar ␣-retaining mechanisms [1][2][3][4]. CGTases act on oligomers or polymers of glucose, catalyzing four reactions: cyclization, disproportionation, coupling and hydrolysis [5].…”

Chitosan and β-Cyclodextrin-epichlorohydrin Polymer Composite Film as a Plant Healthcare Material for Carbendazim-Controlled Release to Protect Rape against Sclerotinia sclerotiorum (Lib.) de Bary

“…In the pharmaceutical, cosmetics, and food industries, β-cyclodextrin (β-CD) either in solution or solid phase has been intensively focused on because of its cavity of suitable size which could non-covalently encapsulate guest molecules with special functionalities [11,12]. Owing to the formation of inclusion complexes, β-CD could improve the solubility, stability, and bioavailability of drugs, reduce the volatility of cosmetic perfumes, and mask unpleasant odors of functionalized molecules [13].…”