Human ERV3-1 env protein expression in various human tissues and tumours

Kang, Yun‐Jeong; Jo, Jin-Ok; Ock, Mee Sun; Chang, Hee-Kyung; Baek, Kyung‐Wan; Lee, Ja-Rang; Choi, Yung Hyun; Kim, Wun-Jae; Leem, Sun‐Hee; Kim, Heui‐Soo; Cha, Hee‐Jae

doi:10.1136/jclinpath-2013-201841

Cited by 12 publications

(11 citation statements)

References 8 publications

(2 reference statements)

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“…We also compared the expression of ERV3-1 between normal and tumour tissues to study the relationship between ERV3-1 and tumour formation. ERV3-1 was highly expressed in certain types of tumours, including lung adenocarcinoma, renal cell carcinoma, papillary carcinoma, hepatocellular carcinoma and adenocarcinoma in the gastrointestinal tract 13. Although a tissue microarray of various tissues shows the expression profile at a glance, it has weaknesses such as an insufficient number of samples and cannot compensate for individual variation in expression.…”

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confidence: 99%

“…Background staining was measured by immunostaining all slides without primary antibody or rabbit IgG and we also confirmed antibody specificity previously by western blot analysis 13. For protein expression assessment, staining intensity was scored as negative (0 point), weak (1 point), moderate (2 points) or strong (3 points).…”

mentioning

confidence: 99%

“…Western blot analysis was conducted as previously described 13. Briefly, 50 μg of tissue extract was prepared using PRO-PREPTM Protein Extraction Solution (Intron Biotechnology, Kyunggi, Korea) and separated by electrophoresis on a Novex 4%–12% Bis–Tris gel (Invitrogen, Carlsbad, California, USA).…”

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confidence: 99%

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Elevation of human ERV3-1 env protein expression in colorectal cancer

Lee

Kang

et al. 2014

J Clin Pathol

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confidence: 99%

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Elevation of human ERV3-1 env protein expression in colorectal cancer

Lee

Kang

et al. 2014

J Clin Pathol

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“…HERV-R (ERV3-1) was highly expressed in normal organs and was significantly increased in various tumors, including lung adenocarcinoma, renal cell carcinoma, papillary carcinoma, hepatocellular carcinoma, and adenocarcinoma in the gastrointestinal tract [24]. Here, we analyzed and compared various HERV env gene mRNA expressions in the blood of 49 breast cancer patients to identify blood HERV env gene expression and to examine the potential use of HERVs as a breast cancer detection marker.…”

Section: Introductionmentioning

confidence: 99%

Expression of Human Endogenous Retrovirus env Genes in the Blood of Breast Cancer Patients

Rhyu

Kang

Ock

et al. 2014

IJMS

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Human endogenous retroviruses (HERV) env proteins have been recently reported to be significantly up-regulated in certain cancers. Specifically, mRNA and protein levels of HERV-K (HML-2) are up-regulated in the blood plasma or serum of breast cancer patients. Here, we collected blood samples of 49 breast cancer patients and analyzed mRNA expressions of various HERVs env genes including HERV-R, HERV-H, HERV-K, and HERV-P by real-time PCR. The expression of env genes were significantly increased in the blood of primary breast cancer patients but were decreased in patients undergoing chemotherapy to a similar level with benign patients. When we compared the group currently undergoing chemotherapy and those patients undergoing chemotherapy simultaneously with radiotherapy, HERVs env genes were reduced more in the chemotherapy only group, suggesting that chemotherapy is more effective in reducing HERV env gene expression than is radiotherapy. Among chemotherapy groups, HERV env gene expression was the lowest in the taxotere- or taxol-treated group, suggesting that taxotere and taxol can reduce HERVs env expression. These data suggest the potential to use HERVs env genes as a diagnosis marker for primary breast cancer, and further studies are needed to identify the mechanism and physiological significance of the reduction of HERV env gene expression during chemotherapy.

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“…9 ERV3-1 Env expression at the protein level has been detected in various cancerous human tissues; this implies an association between ERV3-1 and tumor formation. 25 However, whether the env gene products are functionally conserved in various primate species is not well understood.…”

Section: Introductionmentioning

confidence: 99%

Short Communication: Expression Profiles of Endogenous Retroviral Envelopes inMacaca mulatta(Rhesus Monkey)

Cha

Imai

et al. 2014

AIDS Research and Human Retroviruses

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Endogenous retroviruses (ERVs), which are footprints of ancient germline infections, were inserted into the genome during the early stages of primate evolution. Human endogenous retroviruses (HERVs) occupy approximately 8% of the human genome. Although most ERV genes are defective, with large deletions, stop codons, and frameshifts in their open reading frames (ORFs), some full-length sequences containing long ORFs are expressed in several tissues and cancers. Several envelope glycoproteins that are encoded by env genes have retained some characteristics of their ancestral infectious viruses. These glycoproteins play essential physiological roles in the organs in which they are expressed. Previous studies have demonstrated the expression of ERV env at the mRNA level in cells and tissues rather than at the protein level, which is more difficult to detect. However, it is not known whether Env is functionally conserved in primates. To understand the possible role of Env in primates, we examined the expression of the env genes of four ERVs (ERV-R, -K, -W, and -FRD) at the protein as well as mRNA levels in various tissues of the rhesus monkey. The ERV env gene products were observed at moderate to high levels in each tissue that was examined and showed tissue-specific expression patterns. Our data suggest a biologically important role for retroviral proteins in healthy tissues of the rhesus monkey.

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Human ERV3-1 env protein expression in various human tissues and tumours

Cited by 12 publications

References 8 publications

Elevation of human ERV3-1 env protein expression in colorectal cancer

Elevation of human ERV3-1 env protein expression in colorectal cancer

Expression of Human Endogenous Retrovirus env Genes in the Blood of Breast Cancer Patients

Short Communication: Expression Profiles of Endogenous Retroviral Envelopes inMacaca mulatta(Rhesus Monkey)

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