Coordinated Transcriptional Regulation of Hspa1a Gene by Multiple Transcription Factors: Crucial Roles for HSF-1, NF-Y, NF-κB, and CREB

Sasi, Binu K.; Sonawane, Parshuram J.; Gupta, Vinayak; Sahu, Bhavani S.; Mahapatra, Nitish R.

doi:10.1016/j.jmb.2013.09.008

Cited by 46 publications

(41 citation statements)

References 64 publications

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“…Therefore, instead of the effects on DREB2A, DPB3-1 (NF-YC10) may positively regulate the expression of HsfA2 together with HsfA1s. Other studies in human and other animal cells suggested that an NF-Y trimer induces the transcription of heat stress-inducible genes together with heat shock factors (Li et al, 1998;Imbriano et al, 2001;Sasi et al, 2014). Further analysis is necessary to confirm whether Arabidopsis DPB3-1 and other NF-Y subunits form a transcriptional complex with HsfA1s to regulate HsfA2 expression.…”

Section: Nf-ya (mentioning

confidence: 94%

Arabidopsis DPB3-1, a DREB2A Interactor, Specifically Enhances Heat Stress-Induced Gene Expression by Forming a Heat Stress-Specific Transcriptional Complex with NF-Y Subunits

et al. 2014

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Section: Nf-ya (mentioning

confidence: 94%

Arabidopsis DPB3-1, a DREB2A Interactor, Specifically Enhances Heat Stress-Induced Gene Expression by Forming a Heat Stress-Specific Transcriptional Complex with NF-Y Subunits

et al. 2014

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“…It was determined that HSPA1A was induced significantly more by treatment with CDDO-Im than with CDDO-Me. HSPA1A has been shown in many disease models to be highly protective, and induction of this gene has been related to ischemia/reperfusion injuries and activation of the Nrf2 pathway [57,58]. The difference in expression levels between the CDDO-Im and CDDO-Me groups could partially explain the apparent difference in cytoprotection.…”

Section: Discussionmentioning

confidence: 99%

Cytoprotection of Human Endothelial Cells from Oxidant Stress with CDDO Derivatives: Network Analysis of Genes Responsible for Cytoprotection

et al. 2015

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Aim: To identify drugs that may reduce the impact of oxidant stress on cell viability. Methods: Human umbilical vein endothelial cells were treated with 200 nmol/l CDDO-Im (imidazole) and CDDO-Me (methyl) after exposure to menadione and compared to vehicle-treated cells. Cell viability and cytotoxicity were assessed, and gene expression profiling was performed. Results: CDDO-Im was significantly more cytoprotective and less cytotoxic (p < 0.001) than CDDO-Me. Although both provided cytoprotection by induction of gene transcription, CDDO-Im induced more genes. In addition to a higher induction of the key cytoprotective gene heme oxygenase-1 (38.9-fold increase for CDDO-Im and 26.5-fold increase for CDDO-Me), CDDO-Im also induced greater expression of heat shock proteins (5.5-fold increase compared to 2.8-fold for CDDO-Me). Conclusions: Both compounds showed good induction of heme oxygenase, which largely accounted for their cytoprotective effect. Differences were detected in cytotoxicity at higher doses, indicating that CDDO-Me was more cytotoxic than CDDO-Im. Significant differences were detected in the ability of CDDO-Im and CDDO-Me to affect differential gene transcription. CDDO-Im induced more genes than did CDDO-Me. The source of the differences in gene expression patterns between CDDO-Im and CDDO-Me was not determined but may be important in long-term use of this class of drugs.

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“…The expression of many HSP genes depends on the concerted action of multiple transcription factors, e.g. members of the V-maf musculoaponeurotic fibrosarcoma oncogene homolog (MAF) family, PAX6, NF-Y, NF-κB, or cAMP-response element-binding protein (CREB) (52, 53). This might explain why the relatively high overexpression of the HSF1 isoforms when compared with the wild type HSF1 amount (see Fig.…”

Section: Discussionmentioning

confidence: 99%

Novel Isoforms of Heat Shock Transcription Factor 1, HSF1γα and HSF1γβ, Regulate Chaperone Protein Gene Transcription

Neueder

Achilli

Moussaoui

et al. 2014

Journal of Biological Chemistry

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Background: HSF1 is the major eukaryotic transcription factor that regulates expression of HSP genes.Results: We identify two novel HSF1 isoforms and show that HSF1 isoforms differentially regulate chaperone gene transcription.Conclusion: HSF1 isoforms work synergistically, and the ratio of HSF1 isoforms determines chaperone gene transcription levels.Significance: Our findings unravel an additional layer of chaperone gene regulation through modulation of HSP expression by HSF1 isoform ratios.

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Coordinated Transcriptional Regulation of Hspa1a Gene by Multiple Transcription Factors: Crucial Roles for HSF-1, NF-Y, NF-κB, and CREB

Cited by 46 publications

References 64 publications

Arabidopsis DPB3-1, a DREB2A Interactor, Specifically Enhances Heat Stress-Induced Gene Expression by Forming a Heat Stress-Specific Transcriptional Complex with NF-Y Subunits

Arabidopsis DPB3-1, a DREB2A Interactor, Specifically Enhances Heat Stress-Induced Gene Expression by Forming a Heat Stress-Specific Transcriptional Complex with NF-Y Subunits

Cytoprotection of Human Endothelial Cells from Oxidant Stress with CDDO Derivatives: Network Analysis of Genes Responsible for Cytoprotection

Novel Isoforms of Heat Shock Transcription Factor 1, HSF1γα and HSF1γβ, Regulate Chaperone Protein Gene Transcription

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