Deficiency of phytochrome B alleviates chilling‐induced photoinhibition in rice

Yang, Jianchao; Li, Meng; Xie, Xiaonan; Han, Guoliang; Sui, Na; Wang, Baoshan

doi:10.3732/ajb.1200574

Cited by 42 publications

(35 citation statements)

References 62 publications

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“…The ELI was clearly decreased in the phyB mutants compared with the WT ( Figure 1C ), indicating that phyB deficiency increased membrane integrity under cold stress. This result is consistent with our previous report that a phyB mutant had a more stable chloroplast structure under cold stress (Yang et al, 2013). MDA is the degradation product of polyunsaturated lipids, and the increased membrane integrity of the phyB mutant can be partly attributed to reduced lipid peroxidation as shown by the reduced MDA content ( Figure 1D ).…”

Section: Discussionsupporting

confidence: 94%

“…MDA is the degradation product of polyunsaturated lipids, and the increased membrane integrity of the phyB mutant can be partly attributed to reduced lipid peroxidation as shown by the reduced MDA content ( Figure 1D ). However, Yang et al (2013) revealed that phyB mutants had higher unsaturated fatty acid contents in their membrane lipids, which also partly contributes to the increased membrane integrity of the phyB mutant (Yang et al, 2013). Therefore, we speculate that phyB deficiency improves membrane integrity in rice, which is an important factor for enhanced cold tolerance in phyB mutants.…”

Section: Discussionmentioning

confidence: 99%

“…Functional analysis revealed that over-expression of OsDREB1A, OsDREB1B , or OsDREB1F in rice and over-expression of OsDREB1D or OsDREB1F in Arabidopsis conferred enhanced cold tolerance in transgenic plants (Dubouzet et al, 2003; Wang et al, 2008; Zhang et al, 2009). PhyB deficiency alleviated chilling-induced photoinhibition in rice, probably through a more stabilized chloroplast structure and higher unsaturated fatty acid content in membrane lipids in the phyB mutant (Yang et al, 2013). Cordeiro et al (2016) observed that OsPIF14 could bind to the OsDREB1B promoter through two N-boxes (CACG(A/C)G) and that the flanking regions of the hexameric core were essential for protein-DNA interaction and stability.…”

Section: Introductionmentioning

confidence: 99%

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Phytochrome B Negatively Affects Cold Tolerance by Regulating OsDREB1 Gene Expression through Phytochrome Interacting Factor-Like Protein OsPIL16 in Rice

Cui

et al. 2016

Front. Plant Sci.

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Cross talk between light signaling and cold signaling has been elucidated in the model plant Arabidopsis and tomato, but little is known about their relationship in rice. Here, we report that phytochrome B (phyB) mutants exhibit improved cold tolerance compared with wild type (WT) rice (Oryza sativa L. cv. Nipponbare). The phyB mutants had a lower electrolyte leakage index and malondialdehyde concentration than the WT, suggesting that they had greater cell membrane integrity and less lipid peroxidation. Real-time PCR analysis revealed that the expression levels of dehydration-responsive element binding protein 1 (OsDREB1) family genes, which functions in the cold stress response in rice, were increased in the phyB mutant under normal and cold stress conditions. PIFs are central players in phytochrome-mediated light signaling networks. To explore the relationship between rice PIFs and OsDREB1 gene expression, we produced overexpression lines of rice PIF genes. OsDREB1 family genes were up-regulated in OsPIL16-overexpression lines, which had improved cold tolerance relative to the WT. Chromatin immunoprecipitation (ChIP)-qPCR assay revealed that OsPIL16 can bind to the N-box region of OsDREB1B promoter. Expression pattern analyses revealed that OsPIL16 transcripts were induced by cold stress and was significantly higher in the phyB mutant than in the WT. Moreover, yeast two-hybrid assay showed that OsPIL16 can bind to rice PHYB. Based on these results, we propose that phyB deficiency positively regulates OsDREB1 expression through OsPIL16 to enhance cell membrane integrity and to reduce the malondialdehyde concentration, resulting in the improved cold tolerance of the phyB mutants.

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Section: Discussionsupporting

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Phytochrome B Negatively Affects Cold Tolerance by Regulating OsDREB1 Gene Expression through Phytochrome Interacting Factor-Like Protein OsPIL16 in Rice

Cui

et al. 2016

Front. Plant Sci.

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“…Transgenic rice plants deficient in PhyB exhibited enhanced chilling-stress tolerance relative to the control. The phyB mutant displayed more intact chloroplasts and an increased accumulation of unsaturated fatty acids after a 24-h chilling-stress treatment, suggesting a role for PhyB in the plant's response to cold stress (Yang et al, 2013). By contrast, transgenic rice (Oryza sativa L. cv.…”

Section: Rice Plants Ectopically Expressing Phytochromementioning

confidence: 97%

Photo-biotechnology as a tool to improve agronomic traits in crops

Gururani

Ganesan

Song

2015

Biotechnology Advances

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“…Furthermore, down‐regulation of PHYB displayed reduced chloroplast damage, improved photosystem II efficiency and enhanced chilling tolerance in rice plants (Yang et al . ). The above reports also suggest that the down‐regulation of PHYB activates abiotic stress tolerance mechanisms.…”

Section: Photomorphogenic Phenotypes For Crop Improvementmentioning

confidence: 97%

Development of transgenic crops based on photo‐biotechnology

Ganesan

Lee

Kim

et al. 2017

Plant Cell & Environment

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The phenotypes associated with plant photomorphogenesis such as the suppressed shade avoidance response and de-etiolation offer the potential for significant enhancement of crop yields. Of many light signal transducers and transcription factors involved in the photomorphogenic responses of plants, this review focuses on the transgenic overexpression of the photoreceptor genes at the uppermost stream of the signalling events, particularly phytochromes, crytochromes and phototropins as the transgenes for the genetic engineering of crops with improved harvest yields. In promoting the harvest yields of crops, the photoreceptors mediate the light regulation of photosynthetically important genes, and the improved yields often come with the tolerance to abiotic stresses such as drought, salinity and heavy metal ions. As a genetic engineering approach, the term photo-biotechnology has been coined to convey the idea that the greater the photosynthetic efficiency that crop plants can be engineered to possess, the stronger the resistance to biotic and abiotic stresses. Development of GM crops based on photoreceptor transgenes (mainly phytochromes, crytochromes and phototropins) is reviewed with the proposal of photo-biotechnology that the photoreceptors mediate the light regulation of photosynthetically important genes, and the improved yields often come with the added benefits of crops' tolerance to environmental stresses.

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Deficiency of phytochrome B alleviates chilling‐induced photoinhibition in rice

Abstract: Phytochrome B deficiency in rice with more stabilized chloroplast structure and higher USFA content in membrane lipids could alleviate chilling-induced photoinhibition.

Cited by 42 publications

References 62 publications

Phytochrome B Negatively Affects Cold Tolerance by Regulating OsDREB1 Gene Expression through Phytochrome Interacting Factor-Like Protein OsPIL16 in Rice

Phytochrome B Negatively Affects Cold Tolerance by Regulating OsDREB1 Gene Expression through Phytochrome Interacting Factor-Like Protein OsPIL16 in Rice

Photo-biotechnology as a tool to improve agronomic traits in crops

Development of transgenic crops based on photo‐biotechnology

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