“…costalimai, T. deaneorum, T. guazu, T. jatai, T. jurbergi, T. matogrossensis, T. vandae, and T. williami (Schofield and Galvão, 2009;Obara et al, 2012;Gonçalves et al, 2013). Gardim et al (2013), in their phylogenetic reconstruction based on COI, Cytb, and 16S, did not identify the matogrossensis subcomplex as a monophyletic group, and placed its species into three different clades: 1) T. jurbergi + T. matogrossensis + T. vandae; 2) T. guasayana as the sister group of clade 1; and 3) T. williami + T. guazu. To further clarify the relationships of these species, the present study aims to describe spermatogenesis in T. vandae and the nucleolar behavior T. vandae and T. williami, with a cytotaxonomic focus.…”