24 h observation of a single HeLa cell by impedance measurement and numerical modeling

Tsai, Sung-Lin; Wang, Min-Haw

doi:10.1016/j.snb.2016.01.107

Cited by 23 publications

(11 citation statements)

References 30 publications

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“…Electrical impedance spectroscopy has been utilized in combination with microfluidics as an emerging label-free technology with good efficiency and sensitivity [19,20] to differentiate cell populations [21–24], single droplets [25], and diseased blood cells [26,27] as well as to monitor cellular pathological conditions [28]. This method is based on cellular dielectric properties [19], size and composition [26,29,30] and does not require any biochemical and fluorescence markers.…”

Section: Introductionmentioning

confidence: 99%

Electrical impedance microflow cytometry with oxygen control for detection of sickle cells

Liu

Qiang

Álvarez

et al. 2018

Sensors and Actuators B: Chemical

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Polymerization of intracellular sickle hemoglobin induced by low oxygen tension has been recognized as a primary determinant of the pathophysiologic manifestations in sickle cell disease. Existing flow cytometry techniques for detection of sickle cells are typically based on fluorescence markers or cellular morphological analysis. Using microfluidics and electrical impedance spectroscopy, we develop a new, label-free flow cytometry for non-invasive measurement of single cells under controlled oxygen level. We demonstrate the capability of this new technique by determining the electrical impedance differential of normal red blood cells obtained from a healthy donor and sickle cells obtained from three sickle cell patients, under normoxic and hypoxic conditions and at three different electrical frequencies, 156 kHz, 500 kHz and 3 MHz. Under normoxia, normal cells and sickle cells can be separated completely using electrical impedance at 156 kHz and 500 kHz but not at 3 MHz. Sickle cells, intra-patient and inter-patient show significantly different electrical impedance between normoxia and hypoxia at all three frequencies. This study shows a proof of concept that electrical impedance signal can be used as an indicator of the disease state of a red blood cell as well as the cell sickling events in sickle cell disease. Electrical impedance-based microflow cytometry with oxygen control is a new method that can be potentially used for sickle cell disease diagnosis and monitoring.

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Section: Introductionmentioning

confidence: 99%

Electrical impedance microflow cytometry with oxygen control for detection of sickle cells

Liu

Qiang

Álvarez

et al. 2018

Sensors and Actuators B: Chemical

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“…In lower frequencies, cell membrane is immune to the electric field. As frequency increases, currents start to penetrate the cytosol and thus the cytoplasm and the membrane are represented by a resistor and a capacitor respectively, that constitute a circuit model [2]. Then, the complex impedance of the system can be calculated in Equation 1…”

Section: Theorymentioning

confidence: 99%

“…The most common impedance-related measurements are known as electrochemical impedance spectroscopy (EIS) and are widely used for many different forms of analysis [1]. EIS measurements are efficient for the characterization of single cells on the basis of cellular electrical response over a particular frequency range and also of cellular physiological responses over a long period of time [2]. In addition, the overall biological property of a cell is related to the electrical properties of cytoplasm and the cell membrane [3,4].…”

Section: Introductionmentioning

confidence: 99%

Impedance Study of Dopamine Effects after Application on 2D and 3D Neuroblastoma Cell Cultures Developed on a 3D-Printed Well

et al. 2019

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In this work, the assessment of the interactions of a bioactive substance applied to immobilized cells in either a two-dimensional (2D) or three-dimensional (3D) arrangement mimicking in vivo tissue conditions is presented. In particular, dopamine (DA) was selected as a stimulant for the implementation of an impedance analysis with a specific type of neural cells (murine neuroblastoma). The aim of this study was the extraction of calibration curves at various frequencies with different known dopamine concentrations for the description of the behavior of dopamine applied to 2D and 3D cell cultures. The results present the evaluation of the mean impedance value for each immobilization technique in each frequency. The differential responses showed the importance of the impedance when frequency is applied in both 2D and 3D immobilization cases. More specifically, in 2D immobilization matrix impedance shows higher values in comparison with the 3D cell culture. Additionally, in the 3D case, the impedance decreases with increasing concentration, while in the 2D case, an opposite behavior was observed.

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“…Measurement of electrical cell substrate impedance spectroscopy (ECIS) of cells is of equal importance, since information that cannot be obtained from mechanical response of cells, such as information related to cell adhesion, number of cells, growth and cell-substrate interaction, can be obtained from ECIS [13]. There have been various reports of ECIS being used to measure properties of various kinds of cells [14][15][16], including cardiomyocytes [17][18][19]. ECIS is a non-invasive and label-free method and real-time information on cell behavior can be obtained.…”

Section: Introductionmentioning

confidence: 99%

Polymer-Based Functional Cantilevers Integrated with Interdigitated Electrode Arrays—A Novel Platform for Cardiac Sensing

2020

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Heart related ailments are some of the most common causes for death in the world, and some of the causes are cardiac toxicity due to drugs. Several platforms have been developed in this regard over the years that can measure electrical or mechanical behavior of cardiomyocytes. In this study, we have demonstrated a biomedical device that can simultaneously measure electrophysiology and contraction force of cardiomyocytes. This dual-function device is composed of a photosensitive polymer-based cantilever, with a pair of metal-based interdigitated electrodes on its surface, such that the cantilever can measure the contraction force of cardiomyocytes and the electrodes can measure the impedance between cells and substrate. The cantilever is patterned with microgrooves so that the cardiomyocytes can align to the cantilever in order to make a higher cantilever deflection in response to contraction force. Preliminary experimental results have identified the potential for use in the drug-induced cardiac toxicity tests, and further optimization is desirable to extend the technique to various bio-sensor areas.

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24 h observation of a single HeLa cell by impedance measurement and numerical modeling

Cited by 23 publications

References 30 publications

Electrical impedance microflow cytometry with oxygen control for detection of sickle cells

Electrical impedance microflow cytometry with oxygen control for detection of sickle cells

Impedance Study of Dopamine Effects after Application on 2D and 3D Neuroblastoma Cell Cultures Developed on a 3D-Printed Well

Polymer-Based Functional Cantilevers Integrated with Interdigitated Electrode Arrays—A Novel Platform for Cardiac Sensing

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