Sheep colon acellular matrix: Immunohistologic, biomechanical, scanning electron microscopic evaluation and collagen quantification

Kajbafzadeh, Abdol‐Mohammad; Masoumi, Ahmad; Hosseini, Mohammad; Borjian, Mohammad Amin; Akbarzadeh, Aram; Mohseni, Mohammad Javad

doi:10.1016/j.jbiosc.2013.07.006

Cited by 13 publications

(7 citation statements)

References 33 publications

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“…SEM images exposed the difference of actuation effectiveness between the decellularization solutions, where G3 and G5 samples, using HP, kept the structural appearance on remained pulp (looked like it was corroded continually), while the G2 and G4 samples, exposed to SH, showed a damaged appearance of the pulp, looked like it was frayed [24,25]. …”

Section: Resultsmentioning

confidence: 99%

Tooth Tissue Engineering: Tooth Decellularization for Natural Scaffold

et al. 2016

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Aim:Tissue engineering is a multidisciplinary science that aims to produce replacement organs and biological substitutes. One of the techniques involves decellularizing a biological organ without altering its structure. One challenge is how to demonstrate which method would be better for this process.Methodology:Fifty premolar teeth were divided into five groups: G1 (control): solution of 10% formaldehyde; G2: phosphate buffer saline (PBS), 28 g of tetrasodium ethylenediaminetetraacetic (EDTA), sodium hypochlorite 2.5% (SH); G3: PBS, EDTA and 40v hydrogen peroxide (HP); G4: PBS, EDTA, SH, enzymatic detergent (ED); and G5: PBS, EDTA, HP, ED. Each group was analyzed by scanning electron microscopy (SEM), x-ray, measured weights and color and received statistical analysis.Conclusion:This study demonstrated that G5 was the most appropriate method to obtain a natural scaffold.

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Section: Resultsmentioning

confidence: 99%

Tooth Tissue Engineering: Tooth Decellularization for Natural Scaffold

et al. 2016

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“…Furthermore, the tissue‐engineered structures could be used in studies investigating the physiological and pathophysiological mechanisms. Previously, our research group introduced an efficient ovine colon decellularization protocol to produce bio‐compatible scaffolds (Kajbafzadeh et al, 2014). Also, we demonstrated the efficacy of rat colon bio‐scaffolds for bladder muscular wall regeneration (Kajbafzadeh et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

In vivo application of decellularized rat colon and evaluation of the engineered scaffolds following 9 months of follow‐up

Kajbafzadeh

Fendereski

Khorramirouz

et al. 2020

Cell Biology International

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“…However, donor deficiency and complications such as portal hypertension, and portal vein embolization have restricted the widespread application of this protocol (Association, ; Pepper et al, ). Tissue engineering, which is a source of obtainable tissues to replace malfunctioning or degenerative tissues (Griffith & Naughton, ), has shown promising results in the regeneration of various tissues such as liver (Baptista et al, ), trachea (Kajbafzadeh et al, ), bladder (Kajbafzadeh et al, ), esophagus (Poghosyan et al, ), small intestine (Belchior et al, ), and colon (Kajbafzadeh et al, ). Tissue engineering appears to be a potential novel alternative therapy for insulin‐dependent diabetes mellitus in addition to an immunosuppression‐free state after transplantation, which could alleviate inevitable consequences of conventional therapies for diabetes.…”

Section: Introductionmentioning

confidence: 99%

“…However, donor deficiency and complications such as portal hypertension, and portal vein embolization have restricted the widespread application of this protocol (Association, 2006;Pepper, Gala-Lopez, Ziff, & Shapiro, 2013). Tissue engineering, which is a source of obtainable tissues to replace malfunctioning or degenerative tissues (Griffith & Naughton, 2002), has shown promising results in the regeneration of various tissues such as liver (Baptista et al, 2011), trachea (Kajbafzadeh et al, 2015), bladder (Kajbafzadeh et al, 2016), esophagus (Poghosyan et al, 2016), small intestine (Belchior, Sogayar, & Grikscheit, 2014), and colon (Kajbafzadeh et al, 2014).…”

mentioning

confidence: 99%

Application of a novel bioreactor for in vivo engineering of pancreas tissue

Hashemi

Pasalar

Soleimani

et al. 2017

Journal Cellular Physiology

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Type 1 diabetes is characterized by autoimmune destruction of pancreatic cells. Organ transplantation is an acceptable treatment for native organ failure. However, it is associated with several problems due to a number of reasons, such as the lack of appropriate donors and immunosuppression. In our present study, a novel model is presented for in vivo recellularization of acellular pancreas by implanting between the host pancreas and the adjacent omental flap. In this study, the pancreases were harvested and cannulated via the common bile duct and then, the scaffolds were acellularized by a detergent-based protocol. After that, the abdomens of 35 rats were opened and the spleen was extracted with the adjacent omentum, and placed outside the abdomen. The acellularized scaffold was stretched over the host pancreas and the omentum was wrapped around it to make a sandwich-like structure, which was then fixed with Chromic Sutures 6-0 and marked with Prolene 4-0 on four sides. All samples were biopsied at 14, 30, 60, 90, and 120 days post-transplantation. The result showed marked recellularization of acellularized pancreas with visible neovascularization and neoβ-cells with minimal inflammatory response. This study provides a new approach to produces a normal-like pancreas by allograft transplantation for pancreas tissue engineering. We observed that in vivo transplantation of acellularized pancreas can promote recellularization, proliferation, and differentiation by blood circulation. These findings support that in vivo studies can contribute to finding faster solutions for the treatment of diabetes.

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Sheep colon acellular matrix: Immunohistologic, biomechanical, scanning electron microscopic evaluation and collagen quantification

Cited by 13 publications

References 33 publications

Tooth Tissue Engineering: Tooth Decellularization for Natural Scaffold

Tooth Tissue Engineering: Tooth Decellularization for Natural Scaffold

In vivo application of decellularized rat colon and evaluation of the engineered scaffolds following 9 months of follow‐up

Application of a novel bioreactor for in vivo engineering of pancreas tissue

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