Importance of microbial natural products and the need to revitalize their discovery

Demain, Arnold L.

doi:10.1007/s10295-013-1325-z

Cited by 360 publications

(242 citation statements)

References 115 publications

(113 reference statements)

Supporting

Mentioning

216

Contrasting

Unclassified

Order By: Relevance

“…Natural products (NPs) signify large and diverse secondary metabolites with a comprehensive choice of biological activities those have established with their numerous practices, particularly in human and veterinary and also in agriculture [1][2][3]. NPs are derived from microorganisms, marine animals, and also from plant sources.…”

Section: Introductionmentioning

confidence: 99%

History of Indian Traditional Medicine: A Medical Inheritance.

Adhikari¹,

Paul

2018

Asian J Pharm Clin Res

View full text Add to dashboard Cite

Objective: Indian Traditional Medicine, the foundation of age-old practice of medicine in the world, has played an essential role in human health care service and welfare from its inception. Likewise, all traditional medicines are of its own regional effects and dominant in the West Asian nations; India, Pakistan, Tibet, and so forth, East Asian nations; China, Korea, Japan, Vietnam, and so forth, Africa, South and Central America. This article is an attempt to illuminate Indian traditional medical service and its importance, based on recent methodical reviews.Methods: Web search engines for example; Google, Science Direct and Google Scholar were employed for reviews as well as for meta-analysis. Results:There is a long running debate between individuals, who utilize Indian Traditional Medicines for different ailments and disorders, and the individuals who depend on the present day; modern medicine for cure. The civil argument between modern medicine and traditional medicines comes down to a basic truth; each person, regardless of education or sickness, ought to be educated about the actualities concerning their illness and the associated side effects of medicines. Therapeutic knowledge of Indian traditional medicine has propelled various traditional approaches with similar or different theories and methodologies, which are of regional significance. Conclusion:To extend research exercises on Indian Traditional Medicine, in near future, and to explore the phytochemicals; the current review will help the investigators involved in traditional medicinal pursuit.

show abstract

Section: Introductionmentioning

confidence: 99%

History of Indian Traditional Medicine: A Medical Inheritance.

Adhikari¹,

Paul

2018

Asian J Pharm Clin Res

View full text Add to dashboard Cite

show abstract

“…These predictions have been confirmed experimentally, 7,8 and generalized to actinomycetes and other bacterial taxa with large genomes. [9][10][11][12][13][14][15][16] NPs continue to be important sources of new and novel chemical scaffolds for drug discovery, 17,18 and actinomycetes, particularly Streptomyces species, continue to be the most productive sources. 4,19,20 To access the enormous untapped cryptic SM coding capacity of actinomycetes, it is critical to develop robust approaches to activate gene cluster expression.…”

Section: Introductionmentioning

confidence: 99%

Molecular beacons to identify gifted microbes for genome mining

Baltz¹

2017

J Antibiot

View full text Add to dashboard Cite

Microbial genome mining is a promising technology that is revitalizing natural product discovery. It is now well documented that many bacteria with large genomes, particularly actinomycetes, encode many more secondary metabolites (SMs) than was previously known from their expressed secondary metabolomes. There are effective bioinformatics tools for counting the numbers and nature of SMs, and determining the total coding capacity from finished microbial genomes. However, these methods do not translate well to draft genomes, particularly for large SM gene clusters that contain nonribosomal peptide synthetase (NRPS) or type I polyketide synthase (PKS-I) mega-genes which are prone to fragmentation and misassembly. Small molecular beacons are required to assess the numbers and variety of NRPS, PKS-I and mixed NRPS/PKS-I pathways. In this report, I show that concatenated peptidyl carrier protein-thioesterase di-domains and acyl carrier protein-thioesterase di-domains can be used as multi-probes to survey finished or draft genomes to estimate the numbers of NRPS, PKS-I and mixed NRPS/PKS-I gene clusters to identify gifted actinomycetes.

show abstract

“…[3] The variation of fermentation conditions has been intensively used in order to optimize the production of secondary metabolites in biotechnological processes.…”

mentioning

confidence: 99%

“…[3] The variation of fermentation conditions has been intensively used in order to optimize the production of secondary metabolites in biotechnological processes. [4] Bode et al systematically varied growth conditions in order to induce the formation of diverse secondary metabolites in a selected Streptomyces strain and termed their approach OSMAC (one strain many compounds).…”

mentioning

confidence: 99%

Divalent Transition‐Metal‐Ion Stress Induces Prodigiosin Biosynthesis in Streptomyces coelicolor M145: Formation of Coeligiosins

Morgenstern

Paetz

Behrend

et al. 2015

Chemistry A European J

View full text Add to dashboard Cite

The bacterium Streptomyces coelicolor M145 reacts to transition-metal-ion stress with myriad growth responses, leading to different phenotypes. In particular, in the presence of Co 2 + ions (0.7 mm) S. coelicolor consistently produced a red phenotype. This phenotype, when compared to the wild type, differed strongly in its production of volatile compounds as well as high molecular weight secondary metabolites. LC-MS analysis revealed that in the red phenotype the production of the prodigiosins, undecylprodigiosin and streptorubin B, was strongly induced and, in addition, several intense signals appeared in the LC-MS chromatogram. Using LC-MS/MS and NMR spectroscopy, two new prodigiosin derivatives were identified, that is, coeligiosin A and B, which contained an additional undecylpyrrolyl side chain attached to the central carbon of the tripyrrole ring system of undecylprodigiosin or streptorubin B. This example demonstrates that environmental factors such as heavy metal ion stress can not only induce the production of otherwise not observed metabolites from so called sleeping genes but alter the products from well-studied biosynthetic pathways.In their natural environment, microorganisms such as Actinomyces have to cope with changing and sometimes adverse conditions. Microorganisms not only rapidly adapt under evolutionary pressure [1] but their genomes contain a great repertoire of secondary metabolite genes [2] that can ensure their survival.Actinomyces are most well-known for their secondary metabolites, such as polyketides or non-ribosomal peptides, many of which turned out to be of invaluable pharmaceutical use, for example, as antibiotics. [3] The variation of fermentation conditions has been intensively used in order to optimize the production of secondary metabolites in biotechnological processes.[4] Bode et al. systematically varied growth conditions in order to induce the formation of diverse secondary metabolites in a selected Streptomyces strain and termed their approach OSMAC (one strain many compounds).[5] With the genome sequencing of many Actinomyces such as the model Streptomycete Streptomyces coelicolor A3(2), [6] it became evident that these antibiotic producers comprise a lot more secondary metabolite gene clusters than secondary metabolites that have been identified from them. [2,7] Clearly, the maintenance of secondary metabolite gene clusters and their formation is costly for the producing strains.[8] In contrast to laboratory cultivation under optimized growth conditions, in their natural environment microorganisms have to cope with biotic and abiotic stress factors, for example, competition for space and nutrients. In such scenarios secondary metabolites are likely specifically and flexibly produced to ensure survival under varying and challenging growth conditions. In nature, therefore, microorganisms most likely produce secondary metabolites from "silent gene clusters" to react to their environment. Biotic and abiotic factors influence the microorganisms, leading to morpholo...

show abstract

Importance of microbial natural products and the need to revitalize their discovery

Cited by 360 publications

References 115 publications

History of Indian Traditional Medicine: A Medical Inheritance.

History of Indian Traditional Medicine: A Medical Inheritance.

Molecular beacons to identify gifted microbes for genome mining

Divalent Transition‐Metal‐Ion Stress Induces Prodigiosin Biosynthesis in Streptomyces coelicolor M145: Formation of Coeligiosins

Contact Info

Product

Resources

About