Staphylococcus saprophyticussurface-associated protein (Ssp) is associated with lifespan reduction inCaenorhabditis elegans

Abstract

Staphylococcal lipases have been proposed as pathogenicity factors. In Staphylococcus saprophyticus the surface-associated protein (Ssp) has been previously characterized as a cell wall-associated true lipase. A S. saprophyticus Δssp::ermB mutant has been described as less virulent in an in vivo model of urinary tract infection compared with its wild-type. This is the first report showing that S. saprophyticus induced a lifespan reduction in Caenorhabditis elegans similar to that of S. aureus RN4220. In two S. saprophyticus Δssp::ermB mutants lifespan reduction in C. elegans was partly abolished. In order to attribute virulence to the lipase activity itself and distinguish this phenomenon from the presence of the Ssp-protein, the conserved active site of the lipase was modified by site-directed ligase-independent mutagenesis and lipase activity-deficient mutants were constructed. These results indicate that the Ssp is associated with pathogenicity in C. elegans and one could speculate that the lipase activity itself is responsible for this virulence.

Mycobacterium tuberculosis Primary Infection and Dissemination: A Critical Role for Alveolar Epithelial Cells

“…Extracellular enzymes such as the Streptococcal Surface Enolase (SE) and glyceradehyde-3-phosphate degydrogenase (GAPDH) have evolved to also function as adhesins in some pathogens (Li et al, 2015). The McaP of Moraxella catarrhalis is a lipase/adhesin (Timpe et al, 2003); the Ssp of Staphylococcus saprophytycus is a surface-associated lipase that contributes to virulence (Sakinc et al, 2005, Szabados et al, 2013). The Als family of adhesins in Candida albicans are multifunctional family of proteins (Hoyer and Cota, 2016).…”

Food-derived coagulase-negative Staphylococcus as starter cultures for fermented foods

“…Genome analysis revealed that S. saprophyticus ATCC 15305 T does not possess genes coding for common S. aureus virulence factors such as coagulase and enterotoxins but does contain genes responsible for urease production and adhesion to uroepithelial cells, which might be involved in establishing uncomplicated urinary tract infections (Kuroda et al, 2005 ). While S. saprophyticus surface-associated protein and lipase have been suggested as potential virulence factors (Sakinc et al, 2005 , 2007 ; Szabados et al, 2013 ; Tang et al, 2009 ), no investigations into the pathogenicity of S. saprophyticus in the urinary tract or the expression of potential virulence genes in this environment have been published as yet.…”

Emergence of Staphylococcus lugdunensis as a Cause of Urinary Tract Infection: Results of the Routine Use of MALDI-TOF MS

“…S. saprophyticus is primarily a pathogen of the urinary tract, and the factors involved in the mediation of UTI caused by S. saprophyticus are relatively well studied. S. saprophyticus possesses various types of adhesins such as hemagglutinins, autolysin Ass, and surface-associated lipases such as Ssp, which facilitate effective bacterial adherence to fibronectin and fibrinogen and, hence, colonization of the uroepithelium [12][13][14]. It can also form a bacterial biofilm, which confers an anti-phagocytic and antimicrobial-resistance phenotype and increases the virulence of the bacteria [15].…”