A Transgenic Tri-Modality Reporter Mouse

Yan, Xinrui; Ray, Pritha; Paulmurugan, Ramasamy; Tong, Ricky T.; Gong, Yongquan; Sathirachinda, Ataya; Wu, Joseph C.; Gambhir, Sanjiv S.

doi:10.1371/journal.pone.0073580

Cited by 15 publications

(13 citation statements)

References 34 publications

(36 reference statements)

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“…Tg rats used in this study were generated for in vivo dual-modality imaging of NG2 cells. These rats expressed fLuc and the human oestrogen receptor ligand binding domain (hERL) in NG2 cells, for bioluminescence and positron emission tomography (PET) imaging, respectively 37 . The hERL, which is a fragment of the oestrogen receptor, possesses a binding site for oestrogen or its analogues, including 16α-[ 18 F]-fluoro-17β-estradiol ( 18 F-FES) 38 .…”

Section: Discussionmentioning

confidence: 99%

In vivo monitoring of hair cycle stages via bioluminescence imaging of hair follicle NG2 cells

Tamura

Takata²,

Eguchi

et al. 2018

Sci Rep

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Hair growth occurs periodically in a cycle that consists of three different phases: growth, regression, and resting. The length of each phase is regulated by both intrinsic and extrinsic factors throughout life, and influenced by physiological and pathological conditions. Elongation of the resting phase and shortening of the growth phase occur during physiological ageing and in baldness, respectively. In vivo discrimination of each phase of the hair cycle can be used to research for regeneration of hair follicles as well as to evaluate the efficacy of hair regrowth treatments in the same individual. Here we show that NG2+ epithelial cells in the hair follicles encompass bulge stem cells, and that the number of hair follicle NG2 cells underwent dramatic changes during the hair cycle. Transgenic rats with expression of firefly luciferase gene in NG2 cells were generated to monitor the hair cycle in vivo. Hair follicle NG2 cells were clearly visualized via bioluminescence imaging to study each phase of the hair cycle in the rats, from infancy to old age.

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Section: Discussionmentioning

confidence: 99%

In vivo monitoring of hair cycle stages via bioluminescence imaging of hair follicle NG2 cells

Tamura

Takata²,

Eguchi

et al. 2018

Sci Rep

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“…However, the signal intensities of these reporters have limited sensitivity in vivo , and the cassettes encoding these reporters are large, thus restricting their application when using viral delivery methods that require space to encode transgenes or shRNAs (10–14). Accordingly, a compact multi-modal reporter having enhanced signal intensity is needed for preclinical cancer studies.…”

Section: Introductionmentioning

confidence: 99%

Fluorophore-NanoLuc BRET Reporters Enable Sensitive In Vivo Optical Imaging and Flow Cytometry for Monitoring Tumorigenesis

et al. 2015

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Fluorescent proteins are widely used to study molecular and cellular events, yet this traditionally relies on delivery of excitation light, which can trigger autofluorescence, photoxicity, and photobleaching, impairing their use in vivo. Accordingly, chemiluminescent light sources such as those generated by luciferases have emerged, as they do not require excitation light. However, current luciferase reporters lack the brightness needed to visualize events in deep tissues. We report the creation of chimeric eGFP-NanoLuc (GpNLuc) and LSSmOrange-NanoLuc (OgNLuc) fusion reporter proteins coined LumiFluors, which combine the benefits of eGFP or LSSmOrange fluorescent proteins with the bright, glow-type bioluminescent light generated by an enhanced small luciferase subunit (NanoLuc) of the deep sea shrimp Oplophorus gracilirostris. The intramolecular bioluminescence resonance energy transfer (BRET) that occurs between NanoLuc and the fused fluorophore generates the brightest bioluminescent signal known to date, including improved intensity, sensitivity and durable spectral properties, thereby dramatically reducing image acquisition times and permitting highly sensitive in vivo imaging. Notably, the self-illuminating and bi-functional nature of these LumiFluor reporters enables greatly improved spatio-temporal monitoring of very small numbers of tumor cells via in vivo optical imaging and also allows the isolation and analyses of single cells by flow cytometry. Thus, LumiFluor reporters are inexpensive, robust, non-invasive tools that allow for markedly improved in vivo optical imaging of tumorigenic processes.

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“…Yan et al [ 63 ] assessed the distribution of BM-MSCs in nude mice 10 minutes after induction of myocardial infarction. Cells transfected with a TK gene were injected intramyocardially after induction of the lesion.…”

Section: Preclinical Studiesmentioning

confidence: 99%

In Vivo Tracking of Cell Therapies for Cardiac Diseases with Nuclear Medicine

Moreira

Medeiros

Gutfilen

et al. 2016

Stem Cells International

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Even though heart diseases are amongst the main causes of mortality and morbidity in the world, existing treatments are limited in restoring cardiac lesions. Cell transplantations, originally developed for the treatment of hematologic ailments, are presently being explored in preclinical and clinical trials for cardiac diseases. Nonetheless, little is known about the possible efficacy and mechanisms for these therapies and they are the center of continuous investigation. In this scenario, noninvasive imaging techniques lead to greater comprehension of cell therapies. Radiopharmaceutical cell labeling, firstly developed to track leukocytes, has been used successfully to evaluate the migration of cell therapies for myocardial diseases. A substantial rise in the amount of reports employing this methodology has taken place in the previous years. We will review the diverse radiopharmaceuticals, imaging modalities, and results of experimental and clinical studies published until now. Also, we report on current limitations and potential advances of radiopharmaceutical labeling for cell therapies in cardiac diseases.

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A Transgenic Tri-Modality Reporter Mouse

Cited by 15 publications

References 34 publications

In vivo monitoring of hair cycle stages via bioluminescence imaging of hair follicle NG2 cells

In vivo monitoring of hair cycle stages via bioluminescence imaging of hair follicle NG2 cells

Fluorophore-NanoLuc BRET Reporters Enable Sensitive In Vivo Optical Imaging and Flow Cytometry for Monitoring Tumorigenesis

In Vivo Tracking of Cell Therapies for Cardiac Diseases with Nuclear Medicine

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